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Diluents and cryoprotectants for cryopreservation of Filefish Thamnaconus modestus sperm
레민황,임한규,민병화,장영진,손맹현,이정의 한국양식학회 2008 韓國養殖學會誌 Vol.21 No.1
The present study aimed to find the best diluent and cryoprotectant for sperm cryopreservation of filefish Thamnaconus modestus. Two kinds of artificial seminal plasma (ASP1, ASP2), 0.3 M glucose and marine fish Ringer’s solution (MFRS) were employed as diluent. Dimethyl sulfoxide (DMSO) and methanol as cryoprotectant were selected for sperm cryopreservation. Sperm was diluted at the ratio of 1:3 with diluents containing cryoprotectants and adjusted for final concentration at 5%, 10%, 15% and 20%. Mixed milt was frozen at liquid nitrogen vapor after equilibration for 5 min. The highest motility (40.5±2.8%) and swimming speed (81.5±4.1 μm/s) of frozen/ thawed sperm were observed in ASP1 diluent containing 10% DMSO and in ASP2 containing 15% DMSO, respectively. Results showed that cryopreservation with ASP as diluent and DMSO as cryoprotectant could be adopted for long term storage of filefish sperm.
Milt Properties and Spermatozoa Structure of Filefish(Thamnaconus modestus)
레민황,임한규,민병화,김성연,장영진 한국발생생물학회 2007 발생과 생식 Vol.11 No.3
말쥐치(Thamnaconus modestus) 정액의 물리화학적 특성과 정자의 미세구조에 대하여 분석하였다. 정액량과 정자 농도, spermatocrit는 각각 , , 였다. 정장의 화학적 조성에 있어서 potassium , sodium , chloride , calcium , magnesium , glucose , 총단백질 , 총지질 였으며, 삼투질농도와 pH는 각각 and 였다. 투과형 전자현미경으로 미세구조를 관찰한 결과, 정자는 첨체가 없는 머리 The milt properties of filefish(Thamnaconus modestus) included physical properties of sperm and biochemical properties of seminal plasma. The physical properties of milt were in sperm volume, in sperm concentration and in spermatocrit. The biochemical properties of seminal plasma contained potassium, sodium, chloride, calcium, magnesium, glucose, total protein and total lipid. The osmolality and pH of seminal plasma were and , respectively. The spermatozoon of filefish consisted of three parts: head without acrosome, mid-piece with five mitochondria and flagellum with "9+2" pattern. The head of spermatozoon in longitudinal section was horseshoe-shaped, and long and wide.