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간세포암에서 현미부수체 불안정성과 p53 유전자 이형접합 소실
김준석(Jun Seok Kim),임만섭(Man Sup Lim),김두진(Doo Jin Kim),김주섭(Joo Seop Kim),김관석(Kwan Seok Kim),김홍기(Hong Ki Kim),조성진(Seong Jin Cho),권미정(Mi Jung Kwon),남은숙(Eun Sook Nam),최경찬(Kyung Chan Choi),신형식(Hyung Sik Shin 한국간담췌외과학회 2009 한국간담췌외과학회지 Vol.13 No.3
Purpose : Hepatocellular carcinoma (HCC) shows various molecular and genetic alterations in its development and progression. Recently, microsatelite instability (MSI) and the loss of heterozygosity (LOH), have been postulated as useful prognostic factors in many malignant tumors. LOH is related to the allelic loss of various tumor suppressor genes, however, MSI has been found to be the result of a mismatched DNA pairing. Our objectives were to evaluate MSI and p53 gene LOH and to correlate this to clinicopathological factors. Methods : MSI analysis was performed by using polymerase chain reaction with 5 microsatellite markers (BAT25, BAT26, D2S123, D5S346 and D17S250 recommended in the 1998 NCI International Workshop) on 50 surgically resected tumors. p53 LOH was detected with 4 markers(D17S796, TP53, D17S5, D17S513). Results : MSI and p53 LOH were detected in 30% and 66%, respectively. 18% of HCCs exhibited MSI in 5 NCI-recommended markers and 18% of HCCs demonstrated MSI in 4 p53 markers. MSI was mostly detected in BAT25 and BAT26 markers. MSI was more frequently detected in tumor grade Ⅰ, small HCC, and non-lymphovascular group. For the most aprt, p53 LOH was detected by D17S513 marker(38.1%). p53 LOH results were correlated with higher tumor grade and invasiveness. LOH-High group showed a significant correlation with advanced HCCs and lymphovascular invasion. There was no demonstrated correlation between MSI and p53 LOH was not demonstrated. Conclusion : These results suggest that MSI may be involved to some extent in hepatocarcinogenesis and tumor invasion. Also MSI and p53 gene LOH may be a useful clinical indicator in determining the prognosis among patients with HCC.