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김연비 ( Youn Bee Kim ),김승연 ( Seung Youn Kim ),김재영 ( Jae Young Kim ),한재원 ( Jae Won Han ),유제민 ( Je Min Yoo ),차현정 ( Hyun Jung Cha ),김대재 ( Dae Jae Kim ) 충북대학교 과학교육연구소 2014 과학교육연구논총 Vol.29 No.2
Here we report about the stress response to the exogenous stimuli in the developing cucumber cotyledon genes. We tested about water, salts and cold-stress to the fully developed cucumber cotyledons. The genes are involved in glyoxysomal, peroxisomal, mitochondrial and cytosolic compartments; ICL, gMDH, mMDH, PCK and GLO. Its gene encoding enzymes are essential in lipid mobilization by glyoxylate cycle/gluconeogenesis (ICL, gMDH and PCK) cell respiration by TCA cycle(mMDH), extra photo-respiration in C3 plant photosynthesis(GLO). We used standard RT-PCR technology to examine the gene expression level. As the results of the examination, the gMDH, PCK and mMDH genes were repressed to water and salt-stresses at the 4 day after stress. However, GLO gene did not show any significant changes in gene expression to the water and salt-stresses. In case of cold-stress, gMDH, PCK and mMDH genes showed slight up-regulation in gene expression but not the case in the GLO gene expression as the previous two experiments. It may be explained that water and salt-stresses are the strong exogenous down-regulating stimulus to those gene which repressed ones but cold-stress may not be the same case of the gene expression in cucumber cotyledon development. However, these results are the just pre-mature one to be made final conclusion.