白米飼料로 飼育한 흰쥐의 Plasma 및 Erythrocytes 中의 Free Amino Acid Level에 對하여

김성로(Sung Ro Kim),이현기(Hyun Ki Lee) 한국식품영양과학회 1976 한국식품영양과학회지 Vol.5 No.1

The variations of growth gain and the composition of free amino acid levels in plasma and erythrocytes of young rats(wistar strain ♂) were determined by microbioassay method, feeding diets of rice group and 7% casein group as a control for three weeks.<br/> The results were as follows;<br/> 1. The growth gain of control diet group was higher than the rice diet group.<br/> 2. The contents of free tryptophan, lysine, and threonine levels in plasma and erythrocytes on rats of 7% casein group were higher than the rice group.<br/> 3. In the 7% casein diet group and the rice group, these free amino acids were included more in erythrocytes than in plasma.<br/> 4. Therefore, generally feeding by high protein score diet was included more Try, Lys, Thr in plasma and erythrocytes than feeding by low protein score diet. So the high and low of protein score was assumed by the contents of Try, Lys, Thr in plasma and erythrocytes on rats.

Effect of Dietary Protein Levels on the Manifestation of Gramoxone Toxicity in Rat Liver

김성로(Sung-Ro Kim),이현기(Hyun-Ki Lee),조운복(Un-Bock Jo),최병태(Byung-Tae Choi) 한국식품영양과학회 1992 한국식품영양과학회지 Vol.21 No.3

식이속의 단백질농도가 피리딘계 제초제언 gramoxone의 독성발현에 어떤 영향을 미치는가를 알기 위해 Wistar계 흰쥐 숫놈 36마리 (6~7주령)를 6개의 실험사료군으로 나누어 2주간 사육하며 그 미치는 영향을 조사하였다. 체중증가 상황은 각단백-gramoxone군들의 경우 각 정상단백군에 비해 저조하였으며 저단백-gramoxone 군에서 제일 심하였고 고단백-gramoxone군에서는 유의한 차가 없었다. 간의 지질함량변화는 각단백-gramoxone군들의 경우, 고단백-gramoxone군을 제외하고 각 단백군에 비해 증가하는 경향을 나타내었으며 특히 저단백-gramoxone군에서 가장 심하였다. 간장내 TBA가는 정상단백군, 저단백군, 고단백군 및 고단백-gramoxone군 사이에는 유의차가 없었으나 정상단백-gramoxone군과 저단백-gramoxone군의 경우 전자의 실험군들에 비해 매우 높았다. 정상단백군 및 고단백군의 간에서는 유의한 형태적인 변화가 없었으나 저단백군과 각단백-gramoxone군들에서는 정도의 차이는 있었으나 간조직의 변화가 나타났으며 간세포 지방변화 및 Kupffer세포의 숫적인 증가가 관찰되었다. 특히 간세포 지방변화는 저단백-gramoxone군에서 심하였고 고단백-gramoxone군에서는 현저하지 않았다. 간세포의 glycogen 함량은 각단백-gramoxone군들에서 타군들에 비해 증가하는 경향을 보였으며 저단백-gramoxone군에서 제일 심하였다. Effects of dietary protein levels on the manifestations of the toxicity of gramoxone, a bipyridine herbicide, in the liver of rats were investigated. The addition of gramoxone, with regard to the body weight and feed efficiency ratio of rats, had a more dramatic effect on animals fed a low or intermediate protein diet than for those similarly treated among rats fed a relatively high protein diet. Lipid content in the rat liver tended to increase with the addition of gramoxone into each protein diet, with the exception of the high protein-gramoxone diet. The addition of gramoxone tended to increase hepatic TRA value significantly in rats, especially among those fed the low protein-gramoxone diet or the control-gramoxone diet. Significant morphological changes, including fat changes of hepatic cells and increases in the number of Kupffer cells, were found both in rats fed the low protein diet and those fed any of the gramoxone-treated diets. Fat changes within hepatic cells were found to be especially severe in rats fed the low protein-gramoxone diet. Distributions of glycogen in rat liver appeared to increase in rats fed any of the diets to which gramoxone had been added.

雜穀混食時 흰쥐 血漿中의 遊離 鹽基性 아미노酸(Lys His Arg) 含量에 對하여

배송자(Song-Ja Bae),김성로(Sung-Ro Kim) 한국식품영양과학회 1977 한국식품영양과학회지 Vol.6 No.1

The male rats after weaning were fed with the mixed diets of rice and some cereals for three weeks in a ad-libitum method.<br/> The growthgain of rats were determined by feeding those diets and the contents of free basic amino acids level in plasma were analyzed by amino acid analyzer.<br/> The results were as follows;<br/> 1. Rice diets group was highest in growthgain and weightgain, the second was the mixed of 80% rice-20% barley, and the last was the 80% rice~20% wheat group.<br/> 2. It was similar in the contents of plasma free basic amino acids of every diet group.<br/> The contents of Lys was highest and Arg, His were low in order. The mixed diet of 80% rice-20% barley group was higher than the rice only diet group in the contents of Lys and His. but rice only group was highest in Arg.<br/> The mixed of 80% rice-20% wheat diet group was lowest in the contents of Lys, His, Arg.<br/> Therefore feeding mixed diets of rice and cereals. the contents of Lys was highest, the second was Arg and the last was His in the plasma free basic amino acids level.

Alleviating Effects of Vitamin C on the Gramoxone Toxicity in Rat Liver

조운복(Un-Bock Jo),김성로(Sung-Ro Kim) 한국식품영양과학회 1994 한국식품영양과학회지 Vol.23 No.3

흰쥐의 간조직에 미치는 gramoxone 독성과 비타민 C의 완화효과에 대해 조사하였다. Gramoxone처리군에서 간세포의 혼탁종창과 지방변화가 사육기간이 증가함에 따라 현저하고 혼탁종창보다는 지방변화가 더 현저하였으며, 특히 간소엽의 문맥야주변대 간세포에서 지방변화가 심하였다. Kupffer세포의 수도 증가하여 4주 사육군에서 제일 많이 증가하였다. Gramoxone-비타민 C 처리군에서 간세포의 혼탁종창과 지방변화가 현저히 감소하였다. 간세포의 glycogen 함량은 정상대조군에 비해 gramoxone처리군에서 다소 증가하는 경향을 나타내었으며 특히 지방변화가 많이 일어난 간세포에서 glycogen 함량이 더 많았다. Gramoxone-비타민 C 처리군에서 gramoxone처리군에 비해 간세포의 glycogen 함량이 감소하는 경향을 나타내었다. The behaviour of glycogen and histological changes of hepatic tissues in the liver of rats, aged 6 to 7 weeks, fed 18% casein diet under control, gramoxone and gramoxone+vitamin C(Vt. C) diets has been investigated in a combined histopathological and histochemical studies. Cloudy swelling and fat changes of hepatic cells were observed in the gramoxone group with the duration of feeding time. Fat changes of hepatic cells were observed more obviously than cloudy swelling, especially in the hepatic cells of periportal area. The number of Kupffer's cells increased significantly in the gramoxone group fed for 4 weeks. The cloudy swelling and fat changes decreased obviously in the gramoxone+Vt. C group. Glycogen content of hepatic cells tended to increase slightly in the gramoxone group as compared with the control group. Moreover, glycogen depositions were higher in the hepatic cells where fat changes were obvious. Glycogen content appeared to decrease in the gramoxone+Vt. C group as compared with the gramoxone group. It seems to be that Vt. C has alleviating effects on the gramoxone toxicity in the patterns of glycogen distribution and histological structure of hepatic tissues.

Alleviating Effects of Vitamin C on the Gramoxone Toxicity in the Mucosubstances of Rat Duodenum

조운복(Un-Bock Jo),김성로(Sung-Ro Kim),최병태(Byung-Tae Choi) 한국식품영양과학회 1994 한국식품영양과학회지 Vol.23 No.3

식이속의 비타민 C가 피리딘계 제초제인 gramoxone의 독성발현에 어떤 영향을 미치는 가를 알기위해 6~7주령의 Wistar계 흰쥐 숫놈 32마리를 정상대조군과 실험군으로 나누어 자유급식법으로 4주간 사육하였다. 정상대조군은 2, 3 및 4주 사육군으로 나누고 18% casein식이로 사육하였고 실험군 중 gramoxone처리군도 2, 3 및 4주 사육군으로 나누고 18% casein식이에 0.04% gramoxone을 첨가하여 사육하였으며, gramoxone-비타민 C처리군은 2주 사육군의 gramoxone처리군의 흰쥐에다 18% casein식이에 0.04% gramoxone과 3% 비타민 C를 첨가하여 1주 및 2주 사육하여 3 및 4주 사육군으로 하였다. 십이지장의 조직학적 구조 및 배상세포의 형태변화는 hematoxylin-eosin염색으로, 배상세포내 점액질양과 성상변화는 PAS, alcian blue pH 2.5 및 1.0, alcian blue pH 2.5-PAS염색, alcian blue pH 1.0-PAS염색 및 alden blue pH 1.0-PAS염색 및 aldehyde fuchsin pH 1.7-alcian blue pH 2.5 염색으로 관찰하였다. 정상대조군의 십이지장 융모 및 은와에는 강 sulfomucin과 중성점액질을 분비하는 배상세포와 nonsulfomucin과 중성점액질의 분비하는 배상세포가 섞여 있었으며 융모 배상세포내 점액질양이 은와 배상세포내 점액질양보다 많았으며 사육기간이 증가함에 따라 점액질양이 다소 증가하는 경향을 나타내었다. Gramoxone처리군의 경우 십이지장의 산성점액질 및 중성점액질양이 다 같이 감소되며 사육기간이 증가함에 따라 강 sulfomucin과 중성점액질을 분비하는 배상세포수는 감소하는 반면에 nonsulfomucin과 중성점액질을 분비하는 배상세포는 다소 증가하는 경향을 나타내었으나 gramoxone-비타민 C처리군의 경우 감소된 산성점액질과 중성점액질양이 다시 증가하고 감소 되었던 강 sulfomucin과 중성점액질을 분비하는 배상세포가 다시 증가하였다. 십이지장 배상세포의 형태변화는 gramoxone처리군의 경우 커진 세포, 공포변성된 세포, 세포막이 파괴된 세포 및 미성숙세포가 많이 관찰되었으나 gramoxone-비타민 C처리군의 경우 그 수가 감소되었다. 위의 사실로 보아 비타민 C는 흰쥐 십이지장의 배상 세포형태 및 점액질에 미치는 gramoxone의 독성을 완화시키는 효과가 있음을 알 수 있었다. The effects of vitamin C on the gramoxone toxicity in the duodenal goblet cells of rats were investigated using histochemical methods. Rats in control, gramoxone and gramoxone+vitamin C(Vt. C) group, aged 6 to 7 weeks, were fed 18% casein diet. In the gramoxone group, neutral and acid mucins of the goblet cells in villi and crypts of duodenum tended to decrease as compared with the control group. And the goblet cells secreting nonsulphated mucins tended to increase in number, being usually accompanied by a decrease of the goblet cells secreting sulphated mucins which are prominent in the duodenal mucosa of control group. However, the goblet cells secreting nonsulphated mucins tended to increase in the gramoxone+Vt. C group. Morphological changes of the goblet cells in the gramoxone group were noted vacuolation and demolition of goblet cells, while those changes were not significant in the gramoxone+Vt. C group. It seems to be that Vt. C has alleviating effects on the gramoxone toxicity in secretion and production of the duodenal goblet cells.

Alleviating Effects of Vitamin C on the Gramoxone Toxicity in the Total Lipid Contents, Lipid Peroxidation and Protein Patterns of Rat Liver

조운복(Un-Bock Jo),김성로(Sung-Ro Kim),최병태(Byung-Tae Choi) 한국식품영양과학회 1994 한국식품영양과학회지 Vol.22 No.6

흰쥐의 체중증가량, 식이효율, 간지질함량, 간내 과산화지질 및 단백질양사에 미치는 gramoxone독성과 비타민C의 완화효과에 대해 조사하였다. 체중증가량, 식이효율 및 간무게는 gramoxone처리군이 정상대조군에 비해 현저히 감소되었으나 gramoxone-비타민 C처리군에서 gramoxone처리군에 비해 증가되었다. 간의 지질함량과 TBA가는 정상대조군에 비해 gramoxone처리군에서 현저히 증가하였으며 gramoxone-비타민 C처리군에서 gramoxone처리군에 비해 감소되었다. 간단백질 양상은 gramoxone처리군과 정상대조군에서 별 변화가 없었고 gramoxone처리군의 2주 사육군에서 정상대조군에 비해 단백질량이 현저히 감소되었으나, gramoxone-비타민 C처리군에서 단백질양상에 변화가 왔으며 고분자량의 단백질량은 감소하고 저분자량의 단백질량은 증가하는 경향을 나타내었다. This study was made to determine alleviating effects of vitamin C (Vt C) on the gramoxone toxicity in rats with respect to the growth gain, feed efficiency ratio, total lipid contents, lipid peroxidation and protein patterns in rat liver. Growth gain, feed efficiency ratio and liver weight in the gramoxone group were decreased significantly as compared with the control group, while those values obtained in the gramoxone-Vt C group were increased as compared with the gramoxone group. Lipid contents and TBA value of rat liver of the gramoxone group were increased significantly as compared with the control group. However, those values of the gramoxone+Vt C group were decreased as compared with the gramoxone group. No significant differences were observed in the liver protein patterns between the gramoxone and the control group. The contents of liver protein were decreased significantly in the gramoxone group fed for 2 weeks as compared with the control group. The changes of the liver protein patterns, such as the decrease of high molecular weight protein and the increase of low molecular weight protein were observed in the gramoxone + Vt C group.