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Bacillus amyloliquefaciens k의 생육(生育)과 ${\alpha}-Amylase$ 생산(生産)에 대한 Tunicamycin의 영향(影響)
김기철,산기진수,고월소,전촌학조,Kim, Ki-Cheul,Yamaski, Makari,Takatsuki, Akira,Tamura, Gakuzo 한국응용생명화학회 1981 Applied Biological Chemistry (Appl Biol Chem) Vol.24 No.4
Bacillus amyloliquefaciens K의 생육(生育)과 ${\alpha}-amylase$ 생산(生産)에 대한 Tunicamycin (TM)의 영향(影響)을 검토(檢討)한 결과(結果) 최소생육저지농도(最少生育沮止濃度)는 $0.25{\mu}\textrm{g}/m\ell$이고 생산(生産)된 ${\alpha}-amylase$의 내열성(耐熱性)은 $50^{\circ}C$이었다. TM 첨가량(添加量)이 $1{\mu}\textrm{g}/m\ell$인 액체배지(液體培地)에서 10시간(時間) 진탕배양(振湯培養)할 경우 균(菌)의 형태(形態)는 간형(桿形)에서 부정형(不定形)의 구형(球形)으로 변형(變形)되었으며 24시간배양(時間培養)으로 용해(溶解)되었다. TM와 자외선의 처리로 분리(分離)한 his-, $TM^{\tau}$ 변이주(變異株)의 생육(生育)은 친주(親株)와 같은 경향(傾向) 이었으나 ${\alpha}-amylase$, protease, 및 RNase의 생산량(生産量)은 떨어지는 경향(傾向)이다. The effects of tunicamycin (TM) on the growth and ${\alpha}-amylase$ productivity of B. amyloliquefaciens K were studied. The minimal growth inhibitory concentration was $0.25{\mu}\textrm{g}/m\ell$ and its ${\alpha}-amylase$ was stable up to $50^{\circ}C$. When the saking culture with $1{\mu}\textrm{g}/m\ell$ of Tunicamycin caused the change of cell shape from form rod to irregular circular form and the mycelium lysis. the grow th of His-, $TM^{\tau}$ mutant obtained by treatment of TM and ultraviolet ray was similar to that of the parent strain, but the productivity of ${\alpha}-amylase$, protease, and RNase was lower than that of the parent.
Bacillus amyloliquefaciens k 의 생육과 α - Amylase 생육에 대한 Tunicamycin 의 영향
전촌학조,김기철,산기진수,고월소 한국농화학회 1981 Applied Biological Chemistry (Appl Biol Chem) Vol.24 No.4
The effects of tunicamycin (TM) on the growth and α-amylase productivity of B. amyleliucfaciens K were studied. The minimal growth inhibitory concentration was 0.25㎍/㎖ and its α-amylase was stable up to 50℃. When the saking culture with 1㎍/㎖ of Tunicamycin oaused the change of cell shape from form rod to irregular circular form and the mycelium lysis. the grow th of His-. TM mutant obtained by treatment of TM and ultraviolet ray was similar to that of the parent strain, but the productivity of α-amylase, protease, and RXase was lower than that of the parent.