http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
마우스 두개골 결손 모델에서 지방줄기세포와 BMP-2의 골 재생 효능 비교
강선웅 ( Sun Woong Kang ),나완근 ( Wan Geun La ),강진묵 ( Jin Muk Kang ),이종호 ( Jong Ho Lee ),박정호 ( Jung Ho Park ),김병수 ( Byung Soo Kim ) 한국조직공학·재생의학회 2007 조직공학과 재생의학 Vol.4 No.3
In vivo bone regeneration can be achieved either by transplantation of osteogenic cells differentiated from adipose-derived stem cells (ADSCs) or by delivery of exogenous bone morphogenetic protein-2 (BMP-2). In this study, we compared the osteogenic potential between ADSCs and BMP-2 in a mouse calvarial defect model. Critical- sized circular calvarial defects were made in nude mice. The mice were randomized into 4 groups (n=6 per group) that were treated with either 1) fibrin glue alone, 2) fibrin glue with BMP-2, 3) fibrin glue with undifferentiated ADSCs, or 4) fibrin glue with osteogenically differentiated ADSCs. Specimens were harvested at 8 weeks after treatment and evaluated radiographically and histologically. Differentiation of human ADSCs prior to transplantation did not affect bone regeneration. Transplantation of ADSCs was not superior to BMP-2 delivery for in vivo bone formation in a mouse calvarial defect model.
아실 글리콜 키토산이 첨가된 폴록사머 혼합 젤의 제조와 특성
김다은(Da Eun Kim),오혜민(Hye Min Oh),강선웅(Sun-Woong Kang),허강무(Kang Moo Huh) 한국고분자학회 2017 폴리머 Vol.41 No.6
폴록사머는 PEO-PPO-PEO 삼중블록 고분자로 수용액에서 온도 조절을 통해 가역적 젤을 형성할 수 있다는 장점으로 인하여 생체의료용 분야에 광범위하게 사용되고 있다. 하지만, 단일 폴록사머 젤은 수성 환경에서의 낮은 물리적 안정성으로 인해 생체재료로서의 적용에 한계가 있었다. 본 연구에서는 아실 글리콜 키토산의 혼합으로 폴록사머 젤의 물리화학적 특성을 개선하고자 하였다. 폴록사머와 아실 글리콜 키토산을 혼합하여 다양한 조성의 하이드로젤을 제조하였고, 온도 변화에 따른 졸-젤 상전이 특성과 물리적 안정성을 비교하였다. 아실 글리콜 키토산을 함유한 폴록사머 젤은 온도감응성과 물리화학적 특성이 향상됨과 더불어 낮은 세포독성을 보임으로써, 폴록사머의 생체재료서의 유용성을 보다 높일 수 있을 것으로 기대된다. Poloxamers, PEO-PPO-PEO triblock copolymers, are well-known thermo-reversible sol-gel transition polymers that have great potential for use in biomedical applications, but have several drawbacks such as the weak mechanical properties and physical stability. In this study, we tried to modify and improve the physicochemical properties of poloxamer gels by preparation of acyl glycol chitosan-containing poloxamer gels. Various poloxamer gels with different compositions were prepared and characterized in terms of thermo-reversible sol-gel transition properties, mechanical properties, and physical stability. These hydrogel systems could demonstrate not only enhanced physicochemical and thermo-responsive properties but also low cytotoxicity, allowing poloxamer gels to extend their potential application area.
추간판 조직재생을 위한 지방유래 기질세포와 수핵세포의 공배양 조건
최은희 ( Eun Hee Choi ),박광숙 ( Kwang Sook Park ),한인보 ( In Bo Han ),강선웅 ( Sun Woong Kang ),전흥재,이수홍 ( Soo Hong Lee ) 한국조직공학·재생의학회 2009 조직공학과 재생의학 Vol.6 No.4
Nucleus pulposus(NP) cells located at intervertebral disc are chondrocyte-like cells secreting cartilagespecific matrix molecules such as type 2 collagen and aggrecan. NP cells transplantation is used for recovery of damaged intervertebral disc. However, it is difficult to obtain sufficient quantity of NP cells from donor for cell therapy. Recently, adipose derived stromal cells(ASCs) as alternative cell sources have been extensively studied for cells therapy because ASCs can differentiate into several lineages including chondrogenesis. In this study, we sought to optimize the co-culture condition of NP cells and human ASCs for ASC differentiation. Media condition for NP cells and ASCs co-culture was determined by comparing proliferation and differentiation. After that, various cell ratios between NP cells and ASCs(0, 25, 50, 75, 100% ASC) were tested to find optimal cell ratio for efficient differentiation of ASCs by histochemical staining, cell proliferation, gene expression and so on. According RT-PCR and staining, differentiation of ASCs was dependent on the media condition and cell ratio between NP cells and ASCs. This demonstrated the appropriate media and the optimized cell ratio between NP cells and ASCs are able to induce efficient differentiation of ASCs that is expected to provide efficient intervertebral disc regeneration in vivo.
세포성장억제제를 처리하지 않은 지지세포로부터 인간배아줄기세포 배양법
황승태 ( Seung Taeh Hwang ),이석준 ( Suk Jun Lee ),강선웅 ( Sun Woong Kang ),이수홍 ( Soo Hong Lee ) 한국조직공학·재생의학회 2009 조직공학과 재생의학 Vol.6 No.14
For clinical applications of human embryonic stem cells (hESC), it is critical to develop hESCs culture techniques perfectly excluding animal feeder contamination generated by conventional hESCs culture system. Previously, we have developed novel hESCs culture technique using porous membrane (PM) that not only reduced feeder contamination significantly but also provided isolation of hESCs efficiently. However, feeder cells used for hESCs culture were treated with mitomycin C (MMC) that is not acceptable for clinical application. Therefore, we investigated whether PM system can be further applied for MMC non-treated STO feeder cells. MMC non-treated STO cells were seeded on the bottom of PM and then hESCs were placed on the top of PM. Transfer of hESCs were carried out every 6 days. After 10 passages, pluripotency of hESC was verified through immunostaining of Oct4, SSEA3/4, TRA1-60 and TRA-1-81. According to RT-PCR analysis, the hESC strongly expressed stemness markers such as Nanog and Oct4. Compared to conventional hESC culture technique requiring MMC treatment, PM technique would be more useful to maintain clinical grade hESC by eliminating MMC treatment procedure.
N-아세틸화 글리콜 키토산의 합성과 온도감응성 졸-젤 전이 특성
유호선(Ho Seon You),조명옥(Myeong Ok Cho),조익성(Ik Sung Cho),이정정(Zhengzheng Li),김남홍(Nam-Hong Kim),장미경(Mi-Kyeong Jang),강선웅(Sun-Woong Kang),허강무(Kang Moo Huh) 한국고분자학회 2016 폴리머 Vol.40 No.2
본 연구에서는 수용성 천연고분자인 글리콜 키토산의 N-아세틸화를 통해 새로운 천연고분자 기반의 온도감응성 고분자를 합성하고, 온도감응성을 평가하였다. 글리콜 키토산의 N-아세틸화 반응을 통해 글리콜 키틴 구조의 고분자를 성공적으로 합성하였고, 수용액 상에서 37 °C 주변에서 온도감응성 졸-젤 전이현상이 효과적으로 일어남을 확인하였다. 이러한 졸-젤 전이 특성은 기존의 합성고분자 기반 온도감응성 고분자에 비해 상대적으로 더 낮은 농도(3~16 wt%)에서 보였고, 고분자의 분자량, 아세틸화도, 농도를 변화시킴으로써 효과적으로 조절될 수 있었다. 글리콜 키틴은 우수한 온도감응성 뿐 아니라 낮은 세포독성과 향상된 젤 안정성을 보임으로써, 약물전달, 조직공학, 3차원 세포배양 등 다양한 생체의료용 응용분야에 유용하게 활용될 수 있을 것으로 전망된다. In this study, new polysaccharide-based thermo-sensitive polymers were synthesized and their thermo-sensitive sol-gel transition properties were evaluated. Glycol chitin structures were successfully introduced by N-acetylation reaction of glycol chitosan. The aqueous solutions of glycol chitins demonstrated unique thermo-sensitive sol-gel transition behaviors around 37 °C. Their sol-gel transition properties were observed at a relatively lower concentration range (3~16 wt%) compared to those of typical synthetic polymer based systems and efficiently modulated by varying molecular weight, DA, and concentration. Based on their thermo-sensitivity, low cyto-toxicity, and high gel stability, the glycol chitin polymers could be utilized for various biomedical applications, such as drug delivery, tissue engineering, and 3D cell culture.