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        吳海範,郭皇來,李承錫 又石大學校 1991 論文集 Vol.13 No.-

        The separation of non-chromophoric anions were studied on the ion-cxchange chromatography method by HPLC.a-Naphthalenesulfonic acid(α-NS) was used as a visualization agent as well as ion-exchange agent in this experiments by indirect photometric detection method. Also, several factors affecting to the retention time and the separations of samples were investigated. And, the following results were obtained in this study. 1. These non-chromophoric anions were detected by the indirect photometric detection method at the 285nm for α-NS 2. The capacity factor of samples was influenced by the concentration of α-NS and the strength of organic solvent. 3. Some mixtures of samples were able to separated with good resolution and relatively high sensitivity providing detection limit of 0.1㎍ under optimum condition.

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