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연쇄 구균의 세포벽 단백질 추출물이 림프구 활성의 억제에 미치는 영향
상현숙,임미경 원광대학교 치의학연구소 1995 圓光齒醫學 Vol.5 No.1
The inhibitory effect of cell wall extracts of streptococci, have been investigated to know hostparasite relationship or pathogenesis of abscess formation. Streptococci isolated from the infected root canals were sonicated to get cell wall extracts which have been known as one of the major factors of pyogenesis. Lymphocytes separated by density gradient were stimulated with phytohemagglutinin and exposed to cell wall extracts of Streptococcus sanguis, S. mitis, S. uberis, S. mutans(ATCC 10449), and S. jieealis(ATCC 19433). [^3H]-thymidine uptake of lymphocytes was analyzed with scintillation counter and lactate dehydrogenase (LDH) activity was measured with autochemistry analyzer. S. fieealis had the strongest inhibitory effect, beginning at 100㎍/㎖ concentration of sonic extracts. S. sanguis and S. mitis had inhibitory effect at 300㎍/㎖, while S. uberis and S. mutans showed no inhibitory effect on DNA synthesis even at 300㎍/㎖. Each streptococci showed different inhibitory effect on the DNA synthesis of lymphocytes, of which finding indicated wide spectrum of susceptibility of lymphocytes according to streptococcus spp. There were no significant difference of LD activities between control and each streptococcal extracts. Streptococcal sonic extracts did not affect the morphological findings or number of colonies of activated lymphocytes. These finding suggested the inhibitory effect of sonic extract of streptococci to lymphocytes could be detected by DNA synthesis inhibition, not by cellular membrane damage.
상현숙,정파진,김홍희 朝鮮大學校 口腔生物學硏究所 2002 Oral Biology Research (Oral Biol Res) Vol.26 No.1
The cDNA array technique was employed to analyse gene expression patterns during osteoclast differentiation form mouse bone marrow precursor cells. The following conclusions were drawn from the obtained results. 1. Cytokine receptors such as intrleukin-7 receptor alpha and insulin-like growth factor 1A. hormone precursors such as proopiomelanocortin, signaling molecules such as interferon regulatory factor 1 and PI3-K p85 alpha, transcription factors such as c-fos proto-oncogene, and cell cycle regulatory genes such as TIS21 early response gene increased during osteoclast differentiation. 2 TCRA enhancer-binding factor-interacting protein 1, proliferation-associated protein 1, prothymosin alpha, small inducible cytokine subfamily A member 7, prothymosin beta 4, and rho GDP dissociation inhibitor beta decreased during osteoclast differentiation. These genes have been shown to either mediate signaling pathways or regulate transcription. However, their involvement in osteoclast differentiation has not been reported. Some genes involved in cell cycle regulation such as G2/M-specific cyclin G. G2/M-specific cyclin A2. G2/M-specific cyclin B2. and cyclin-dependent kinase inhibitor 2D also decreased during osteoclastogenesis.
RANKL 에의한 파골세포 분화에 Superfect 와 Tfx의 효과에 관한 연구
상현숙,김홍희,이홍찬 朝鮮大學校 口腔生物學硏究所 2002 Oral Biology Research (Oral Biol Res) Vol.26 No.1
The ability to introduce DNA into mammalian cells provides a powerful means to study the function and control of mammalian genes. Common techniques for gene(DNA) transfer into eukaryotic cells include calcium phosphate, DEAE-Dextran electroporations and more recently, liposome-mediated transfection. When reagents for liposome-mediated transfection were tested with RAW264.7 cells that can differentiate into osteoclast-like cells upon RANKL treatment. Superfect was found to reduce the differentiation whereas TfxTM had no effects. Therefore, TfxTM may be a more suitable reagent fo rintroducing DNA into cells in osteoclastogenesis studies.