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( Mahesh Sapkota ),( Liang Li ),( Hyukjae Choi ),( William H Gerwick ),( Yunjo Soh ) 영남대학교 약품개발연구소 2016 영남대학교 약품개발연구소 연구업적집 Vol.26 No.-
Osteoclasts are unique bone remodeling cells derived from multinucleated myeloid progenitor cells. They play homeostatic vital roles in skeletal modeling and remodeling but also destroy bone masses in many pathological conditions such as osteoporosis and rheumatoid arthritis. Receptor activation of NF-KB li-gand (RANKL) is essential osteclastogenesis. In this study, we investigated the effects of bromo-honaucin A (Br-H A) isolated from Leptolyngbya crossbyana (cyanobacterium). To investigate the me-chanism of the inhibitory effect of Br-H A on osteoclstogenesis. we employed Br-H Ain RANKL-treated murine monocyte/macrophage RAW 264.7 cells for osteoclastic differentiation in-vitro. The inhibitory effects on in-vitro osteoclastogenesis was evaluated by counting the number of Tartarate resistant acid phospatase (TRAP) positive multinucleated cells and by measuring the expression level of osteoclast-specific genes like matrix metalloproteinase 9 (MMP9). cathepsin K (CATH K), GRB2-associated-binding protein 2 (GAB2), c-terminal myc kinase (C-MYC). C-terminal Src kinase (C-SRC) and Microphthalmia-associated transcription factor (MITF). Moreover, Br-H A blocked the resorbing capacity of RAW 264.7 cells on calcium phosphate-coated plates. Finally, Br-H A clearly decreased the expression of Akt and also decreased the activation of ERK. Thus, the study identifies Br-H A as potent inhibitor potentialin the treatment of diseases involving abnormal bone lysis such as osteoporosis. rheumatoid arthritis, and periodontal bone degradation.
( Hyuk Jae Choi ),( Philip J Proteau ),( Tara Byrum ),( William H Gerwick ) 영남대학교 약품개발연구소 2012 영남대학교 약품개발연구소 연구업적집 Vol.22 No.0
An investigation of the oxylipin chemistry of the temperate brown alga Cymathere triplicata led to the isolation of several secondary metabolites, cymatherelactone (1) and cymatherols A-C (2-4), the latter as their methyl ester derivatives (5-7), which contained cyclopentyl, cyclopropyl, epoxide and lactone rings. Their structures were elucidated using a combination of spectroscopic techniques and synthetic chemistry. Cymatherelactone (1), as well as R- and S-Mosher`s esters of its seco acid, exhibited moderate sodium channel blocking activity.
( Han Bing Nan ),( Harald Gross ),( Kerry L. Mcphail ),( Doug Goeger ),( Claudia S. Maier ),( William H. Gerwick ) 한국미생물 · 생명공학회 2011 Journal of microbiology and biotechnology Vol.21 No.9
Two new cyclic depsipeptides wewakamide A (1) and guineamide G (2) have been isolated from the marine cyanobacterium Lyngbya semiplena and Lyngbya majuscula, respectively, collected from Papua New Guinea. The amino and hydroxy acid partial structures of wewakamide A and guineamide G were elucidated through extensive spectroscopic techniques, including HR-FABMS, 1D 1H and 13C NMR, as well as 2D COSY, HSQC, HSQCTOCSY, and HMBC spectra. The sequence of the residues of wewakamide A was determined through a combination of ESI-MS/MS, HMBC, and ROESY. Wewakamide A possesses a β-amino acid, 3-amino-2-methylbutanoic acid (Maba) residue, which has only been previously identified in two natural products, guineamide B (3) and dolastatin D (4). Although both new compounds (1,2) showed potent brine shrimp toxicity, only guineamide G displayed significant cytotoxicity to a mouse neuroblastoma cell line with LC50 values of 2.7 ?M.
( Niclas Engene ),( Erin C Rottacker ),( Jan Kastovsky ),( Tara Byrum ),( Hyuk Jae Choi ),( Mark H Ellisman ),( Jiri Komarek ),( William H Gerwick ) 영남대학교 약품개발연구소 2012 영남대학교 약품개발연구소 연구업적집 Vol.22 No.0
The filamentous cyanobacterial genus Moorea gen. nov., described here under the provisions of the International Code of Botanical Nomenclature, is a cosmopolitan pan-tropical group abundant in the marine benthos. Members of the genus Moorea are photosynthetic (containing phycocyanin, phycoerythrin, allophycocyanin and chlorophyll a), but non-diazotrophic (lack heterocysts and nitrogenase reductase genes). The cells (discoid and 25-80 μm wide) are arranged in long filaments (<10 cm in length) and often form extensive mats or blooms in shallow water. The cells are surrounded by thick polysaccharide sheaths covered by a rich diversity of heterotrophic micro-organisms. A distinctive character of this genus is its extraordinarily rich production of bioactive secondary metabolites. This is matched by genomes rich in polyketide synthase and non-ribosomal peptide synthetase biosynthetic genes which are dedicated to secondary metabolism. The encoded natural products are sometimes responsible for harmful algae blooms and, due to morphological resemblance to the genus Lyngbya, this group has often been incorrectly cited in the literature. We here describe two species of the genus Moorea: Moorea producens sp. nov. (type species of the genus) with 3L(T) as the nomenclature type, and Moorea bouillonii comb. nov. with PNG5-198(R) as the nomenclature type.
Heterologous Production of 4-O-Demethylbarbamide, a Marine Cyanobacterial Natural Product
( Eun Ji Kim ),( Jong Hyun Lee ),( Hyukjae Choi ),( Alban R Pereira ),( Yeon Hee Ban ),( Young Ji Yoo ),( Eunji Kim ),( Je Won Park ),( David H Sherman ),( William H Gerwick ),( Yeo Joon Yoon ) 영남대학교 약품개발연구소 2013 영남대학교 약품개발연구소 연구업적집 Vol.23 No.0
Heterologous expression of the barbamide biosynthetic gene cluster, obtained from the marine cyanobacterium Moorea producens, in the terrestrial actinobacterium Streptomyces venezuelae, resulted in the production of a new barbamidecongener 4-O-demethylbarbamide, demonstrating the potential of this approach for investigating the assembly and tailoring of complex marine natural products.
Rho, Jung-Rae,Subramaniam, Gurusamy,Choi, Hyukjae,Kim, Eun-Hee,Ng, Sok Peng,Yoganathan, K.,Ng, Siewbee,Buss, Antony D.,Butler, Mark S.,Gerwick, William H. American Chemical Society 2015 ORGANIC LETTERS Vol.17 No.6
<P>Gargantulide A (<B>1</B>), an extremely complex 52-membered macrolactone, was isolated from <I>Streptomyces</I> sp. A42983 and displayed moderate activity against MRSA. The planar structure of <B>1</B> was determined using 2D NMR, and its stereochemistry was partially established on the basis of NOESY correlations, <I>J</I>-based configuration analysis, and Kishi’s universal NMR database.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/orlef7/2015/orlef7.2015.17.issue-6/acs.orglett.5b00068/production/images/medium/ol-2015-00068j_0003.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ol5b00068'>ACS Electronic Supporting Info</A></P>