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- 저자 : ( Shiao-hsuan Yang ) (검색결과 1 건)
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Dexamethasone regulates the development and connexin43 expression of rat preantral follicles
( Shiao-hsuan Yang ),( Chueh-ko Yang ),( Cheng-hsuan Wu ),( Horng-der Tsai ) 대한산부인과학회 2016 대한산부인과학회 학술대회 Vol.102 No.-
Objectives: Corticorsterone has been shown to inhibit luteinizing hormone production and the receptors had been found in the follicles. Ovarian follicles represent the basic functional unit of the ovary. In the study we had evaluated the effect of synthetic glucocorticoids dexamethasone (DEX) on the growth (the increase in size), differentiation and connexin 43 of intact preantral follicles in a serum-free culture system. Materials and methods: Preantral follicles with two layer granulosa cells and intact membrane were dissected mechanically from the ovaries of 24-day-old rats and cultured for 72 hours with or without recombinant human follicle- stimulating hormone (FSH) in the presence or absence of increasing concentrations of glucocorticoids. To assess growth rates, follicles were cultured for 72 hours and analyzed for follicle diameter and follicle cell numbers changes. After 72 hours, the follicles were collected and their connexin 43 and inhibin-α contents were determined. Results: Follicles cultured in the absence of FSH grew slowly (around 10% in diameter). Treatment of the follicles with FSH induced an increase in both of follicle size, follicular cell number, and follicle cell differentiation based on increased inhibin-α synthesis. When follicles were cultured with a constant dose of FSH (100 mIU/ml) and increasing doses of dexamethasone (DEX) (0.001-1 μg/ml), the growth rate was reduced by 18-83%. The inhibitory effect by DEX was concentration dependent. The addition of DEX (0.1 μg/ml) decreased FSH-induced follicle cell number. This decrease was associated with decreased expression of proliferating cell nuclear antigen (PCNA), and inhibin-α, a differential marker of preantral follicle. The DEX-induced decrease in the inhibin-α content was also associated with decreased expression of the connexin 43. Conclusion: These results demonstrated that DEX is able to attenuate the FSH-stimulated preantral follicle development.
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