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      저자 : ( Kana Kasai ) (검색결과 1 건)
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      • Intraperitoneal inflammation progress the development of endometriosis in mouse model

        ( Kaoru Keyama ),( Kana Kasai ),( Sumika Matsui ),( Kanako Yoshida ),( Takeshi Kato ),( Minoru Irahara ) 대한산부인과학회 2016 대한산부인과학회 학술대회 Vol.102 No.-

        Objective: Previous studies show that the abdomen inflammation advance formation of endometriosis. The aim of this study is to clarify the intraperitoneal inflammation progress the development of endometriosis in mouse model. Methods: We used C57BL/6J female mice in 8 weeks. Oophorectomy was performed to donor mice. Then estradiol (2 μg/day) was injected subcutaneously for 7 days. The endometrium tissue was removed on 8th day. The tissue and flesh blood(100 μl/body) of donor mice was implanted to recipient mice intraperitoneally as a control group. In another group, LPS(100 μg/body) was injected intraperitoneally one day before the implantation. In the other group, the endometrium tissue , flesh blood (100 μl/body) and LPS(100 μg/body) was implanted at the same day. The abdominal cavity was opened in 15th day, then the number of lesion and the greatest dimension was measured. The endometriosis lesion tissue were diagnosed histologically with HE staining. As a next trial, we conducted the analysis of the concentration of inflammatory cytokines in ascites. We injected LPS (50 μg/body) into the abdominal cavity of mice. Then abdominal irrigation was done with saline(1ml) after 2 hours, 6 hours, 1 day, 3 days, 5 days, 7 days and 10 days of injection. The measurement items are TNFα, MIP-2, and IL-6. Results: The endometrial lesions are significantly formed in the mice which was implanted blood, endometrial tissue, and LPS at the same time. TNFα and IL-6 reached the maximum level in 2 hours, and MIP-2 did in 7days after injection. Conclusion: LPS causes inflammation, and it progresses the development of endometriosis.

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