대학생의 친환경의식과 지속가능발전에 대한 인식 및 녹색소비행동 분석

유지현(Yu, Jihyun),오재림(Oh, Jaelim) 한국산림휴양복지학회 2016 한국산림휴양학회지 Vol.20 No.2

본 연구의 목적은 대학생이 인식하는 친환경의식과 지속가능발전에 대한 인식 및 녹색소비행동을 분석하고 대학생들에게 요구되는 환경교육에 대해 논의하는 것이다. 연구목적을 달성하기 위해, 서울과 경기, 충청지역에 위치한 4년제 대학에 재학 중인 388명을 연구대상으로 설문조사를 실시하였다. 수집된 양적 자료는 PASW 21.0 통계프로그램을 사용하여 기술통계, 상관분석, ANOVA, 회귀분석을 실시하였다. 주요 분석결과는 다음과 같다. 첫째, 성별, 학년별 집단에 따른 측정변수에 유의한 차이가 있는 것으로 나타났다. 둘째, 사범계열과 비사범계열 집단의 차이분석에서 지속가능발전에 대한 인식의 차이는 유의하였으나, 친환경의식과 녹색소비 행동의 인식차이는 유의하지 않았다. 셋째, 친환경의식과 지속가능발전에 대한 인식은 녹색소비행동에 유의한 영향을 미치는 것으로 나타났다. The purpose of this study was to analysis of eco-friendly perception, attitude toward sustainable development and green consumer behavior in Korean undergraduate students. Data were collected from 388 undergraduate students in Seoul, Gyunggi, and Choongchung province. Descriptive statistics, correlations, oneway ANOVA, and Regression were conducted for the purpose of data analysis using the PASW 21.0 program. The major findings of this study were as follows. First, there were significant differences among gender and grade. However, there were no significant differences between teacher college students and non teacher college students except attitude toward sustainable development. The eco-friendly perception and attitude toward sustainable development have significantly effect on green consumer behavior.

A Nucleic-Acid Hydrolyzing Single Chain Antibody Confers Resistance to DNA Virus Infection in HeLa Cells and C57BL/6 Mice

Lee, Gunsup,Yu, Jaelim,Cho, Seungchan,Byun, Sung-June,Kim, Dae Hyun,Lee, Taek-Kyun,Kwon, Myung-Hee,Lee, Sukchan Public Library of Science 2014 PLoS pathogens Vol.10 No.6

<▼1><P>Viral protein neutralizing antibodies have been developed but they are limited only to the targeted virus and are often susceptible to antigenic drift. Here, we present an alternative strategy for creating virus-resistant cells and animals by ectopic expression of a nucleic acid hydrolyzing catalytic 3D8 single chain variable fragment (scFv), which has both DNase and RNase activities. HeLa cells (SCH7072) expressing 3D8 scFv acquired significant resistance to DNA viruses. Virus challenging with Herpes simplex virus (HSV) in 3D8 scFv transgenic cells and fluorescence resonance energy transfer (FRET) assay based on direct DNA cleavage analysis revealed that the induced resistance in HeLa cells was acquired by the nucleic acid hydrolyzing catalytic activity of 3D8 scFv. In addition, pseudorabies virus (PRV) infection in WT C57BL/6 mice was lethal, whereas transgenic mice (STG90) that expressed high levels of 3D8 scFv mRNA in liver, muscle, and brain showed a 56% survival rate 5 days after PRV intramuscular infection. The antiviral effects against DNA viruses conferred by 3D8 scFv expression in HeLa cells as well as an <I>in vivo</I> mouse system can be attributed to the nuclease activity that inhibits viral genome DNA replication in the nucleus and/or viral mRNA translation in the cytoplasm. Our results demonstrate that the nucleic-acid hydrolyzing activity of 3D8 scFv confers viral resistance to DNA viruses <I>in vitro</I> in HeLa cells and in an <I>in vivo</I> mouse system.</P></▼1><▼2><P><B>Author Summary</B></P><P>Most strategies for developing virus-resistant transgenic cells and animals are based on the concept of virus-derived resistance, in which dysfunctional virus-derived products are expressed to interfere with the pathogenic process of the virus in transgenic cells or animals. However, these viral protein targeting approaches are limited because they only target specific viruses and are susceptible to viral mutations. We describe a novel strategy that targets the viral genome itself, rather than viral gene products, to generate virus-resistant transgenic cells and animals. We functionally expressed 3D8 scFv which has both DNase and RNase activities, in HeLa cells and transgenic mice. We found that the transgenic cells and mice acquired complete resistance to two DNA viruses (HSV and PRV) without accumulating the virus, and showed delayed onset of disease symptoms. The antiviral effects against DNA viruses demonstrated in this study were caused by (1) DNase activity of 3D8 scFv in the nucleus, which inhibited DNA replication or RNA transcription and (2) 3D8 scFv RNase activity in the cytoplasm, which blocked protein translation. This strategy may facilitate control of a broad spectrum of viruses, including viruses uncharacterized at the molecular level, regardless of their genome type or variations in gene products.</P></▼2>

A Bivalent Inactivated Vaccine Prevents Enterovirus 71 and Coxsackievirus A16 Infections in the Mongolian Gerbil

Yi Eun-Je,Kim Young-In,Kim Seung-Yeon,Ahn Sung Hyun,Lee Hyoung Jin,Suh Bohyun,Yu Jaelim,Park Jeehye,Lee Yoon Jung,Jung Eunju,Chang Sun-Young 한국응용약물학회 2023 Biomolecules & Therapeutics(구 응용약물학회지) Vol.31 No.3

Hand-foot-and-mouth disease (HFMD) is a viral infectious disease that occurs in children under 5 years of age. Its main causes are coxsackievirus (CV) and enterovirus (EV). Since there are no efficient therapeutics for HFMD, vaccines are effective in preventing the disease. To develop broad coverage against CV and EV, the development of a bivalent vaccine form is needed. The Mongolian gerbil is an efficient and suitable animal model of EV71 C4a and CVA16 infection used to investigate vaccine efficacy following direct immunization. In this study, Mongolian gerbils were immunized with a bivalent inactivated EV71 C4a and inactivated CVA16 vaccine to test their effectiveness against viral infection. Bivalent vaccine immunization resulted in increased Ag-specific IgG antibody production; specifically, EV71 C4a-specific IgG was increased with medium and high doses and CVA16- specific IgG was increased with all doses of immunization. When gene expression of T cell-biased cytokines was analysed, Th1, Th2, and Th17 responses were found to be highly activated in the high-dose immunization group. Moreover, bivalent vaccine immunization mitigated paralytic signs and increased the survival rate following lethal viral challenges. When the viral RNA content was determined from various organs, all three doses of bivalent vaccine immunization were found to significantly decrease viral amplification. Upon histologic examination, EV71 C4a and CVA16 induced tissue damage to the heart and muscle. However, bivalent vaccine immunization alleviated this in a dose-dependent manner. These results suggest that the bivalent inactivated EV71 C4a/CVA16 vaccine could be a safe and effective candidate HFMD vaccine.

A Bivalent Inactivated Vaccine Prevents Enterovirus 71 and Coxsackievirus A16 Infections in the Mongolian Gerbil

( Eun-je Yi ),( Young-in Kim ),( Seung-yeon Kim ),( Sung Hyun Ahn ),( Hyoung Jin Lee ),( Bohyun Suh ),( Jaelim Yu ),( Jeehye Park ),( Yoon Jung Lee ),( Eunju Jung ),( Sun-young Chang ) 한국응용약물학회 2023 Biomolecules & Therapeutics(구 응용약물학회지) Vol.31 No.3

Hand-foot-and-mouth disease (HFMD) is a viral infectious disease that occurs in children under 5 years of age. Its main causes are coxsackievirus (CV) and enterovirus (EV). Since there are no efficient therapeutics for HFMD, vaccines are effective in preventing the disease. To develop broad coverage against CV and EV, the development of a bivalent vaccine form is needed. The Mongolian gerbil is an efficient and suitable animal model of EV71 C4a and CVA16 infection used to investigate vaccine efficacy following direct immunization. In this study, Mongolian gerbils were immunized with a bivalent inactivated EV71 C4a and inactivated CVA16 vaccine to test their effectiveness against viral infection. Bivalent vaccine immunization resulted in increased Ag-specific IgG antibody production; specifically, EV71 C4a-specific IgG was increased with medium and high doses and CVA16-specific IgG was increased with all doses of immunization. When gene expression of T cell-biased cytokines was analysed, Th1, Th2, and Th17 responses were found to be highly activated in the high-dose immunization group. Moreover, bivalent vaccine immunization mitigated paralytic signs and increased the survival rate following lethal viral challenges. When the viral RNA content was determined from various organs, all three doses of bivalent vaccine immunization were found to significantly decrease viral amplification. Upon histologic examination, EV71 C4a and CVA16 induced tissue damage to the heart and muscle. However, bivalent vaccine immunization alleviated this in a dose-dependent manner. These results suggest that the bivalent inactivated EV71 C4a/CVA16 vaccine could be a safe and effective candidate HFMD vaccine.