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RISS 인기검색어
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History of orchid propagation: a mirror of the history of biotechnology
Tim Wing Yam,Joseph Arditti 한국식물생명공학회 2009 Plant biotechnology reports Vol.3 No.1
Orchid seeds are nearly microscopic in size. Because of that, many fanciful theories were proposed for the origin of orchids. Almost 400 years separate the time when orchid seeds were seen for the first time and the development of a practical asymbiotic method for their germination. The seeds were first observed and drawn during the sixteenth century. Seedlings were first described and illustrated in 1804. The association between orchid and fungi was observed as early as 1824, while the requirement for mycorrhiza for seed germination was established in 1899. An asymbiotic method for orchid seed germination was developed in 1921. After Knudson’s media B and C were formulated, orchids growing and hybridization became widespread. Hybrids which early growers may not have even imagined became possible. Orchid seeds are nearly microscopic in size. Because of that, many fanciful theories were proposed for the origin of orchids. Almost 400 years separate the time when orchid seeds were seen for the first time and the development of a practical asymbiotic method for their germination. The seeds were first observed and drawn during the sixteenth century. Seedlings were first described and illustrated in 1804. The association between orchid and fungi was observed as early as 1824, while the requirement for mycorrhiza for seed germination was established in 1899. An asymbiotic method for orchid seed germination was developed in 1921. After Knudson’s media B and C were formulated, orchids growing and hybridization became widespread. Hybrids which early growers may not have even imagined became possible.
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姜鎬德,朴敎秀,Richard B. Hall 동국대학교 생명자원과학대학 연습림 1995 연습림논문집 Vol.- No.4
교잡종 포플러 (Canada blanc, Eugenei, Ⅰ-45/51, Wisconsin #5)의 기내증식에 미치는 요인들이 조사되어졌다. 식물성장 조절물질로서 싸이토키닌류와 오옥신류와의 조합은 뿌리, 줄기, 잎의 시료로부터 기내증식의 효율 증대에 영향을 미치었다. 일반적으로 benzylaminopurine (BA)과 오옥신과의 조합은 Canada blanc 클론의 잎 시료에 매우 효과적 이었고, zeation과 오옥신류와의 조합은 Wisconsin #5 클론의 줄기 시료에 매우 중요하게 작용했다. 각 시료에서 최대의 증식되어진 줄기의 수는 BA 2.0 ㎎/l를 처리 했을때 Canada blanc의 뿌리 시료로 부터 17.60±8.96개가 생산되어졌다. 반면에, Wisconsin #5의 줄기와 Canada blanc의 잎은 zeation/ 2.0㎎/l와 IAA 0.2㎎/l의 조합과/ BA0.5㎎/l와 2.4-D 0.05㎎/l의 조합에서 각각 18.20±3.03개와 17.80±1.92개의 줄기들을 증식시켰다. 특히, 싸이토키닌의 들어있는 배지에 2.4-D의 첨가는 줄기증식을 촉진시켰다.
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