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피부생검 및 조직표본 제작상의 결함과 그 원인에 대한 연구
이종석(Jong Suk Lee),박시룡(See Yong Park),김수남(Soo Nam Kim) 대한피부과학회 1985 대한피부과학회지 Vol.23 No.2
Dermatologist should be aware of the practical importance of proper selection of ski.n lesions for histologic examination, the correct methods for performing biopsy, and steps involved in tissue preparation. They should be acquainted with factors producing various artifacts and ways to prevent these changes. This study was undertaken to investigate the artifacts of 300 dermatopathologic tissue sections which prepared by 3 general hospitals in Seoul in 1983. The results were summarized as follows. [.The percentage of artifacts produced by selection of inapropriate lesion was 4. 0% artifacts during biopsy procedure was 18. 3% inadequate size and depth of sp cimen was 7. 3%, faulty embedding was 12. 0%, tissue tearing or loss was 49. 3% artifactal dermoepidermal separation was 3.0%, linear knife marking was 17.0% thick or thin section was 8. 3% tissue overlapping was 12. 0% uneven stiining or precipitation was 3. 7%, and foreign body contamination was 3, 7% The percentage of good specimen was 21, 7% diagnostic was 56. 3% not diagnostic but suggestive was 8.7% and reading impossible was 13. 3% 3. The major causes of reading impossible specimen which attributed to the procedures of dermatologist were 40.0% to the proccss of technician were 47.5% and to both were 12. 5%
Plant Regeneration Via Organogenesis on Petiole of Centella asiatica (L.) Urban
황백 한국약용작물학회 2006 한국약용작물학회지 Vol.14 No.2
An efficient plant regeneration of C. asiatica was achieved from organogenesis using petiole explants of in vitro plantlet on MS basal medium controled with different plant growth regulators (NAA, 2,4-D, IAA kinetin, and BA). Best results that 50%, efficiency of regeneration per explant for regeneration were obtained with IAA 17.13 μM and BA 8.9 μM. Formation of adventitious shoots via organogenesis from the petiole explant was verified by histological sectioning of plantlets. Regenerated plants were transplanted into soil. An efficient plant regeneration of C. asiatica was achieved from organogenesis using petiole explants of in vitroplantlet on MS basal medium controled with different plant growth regulators (NAA, 2,4-D, IAA kinetin, and BA). Best resultsthat 50%, efficiency of regeneration per explant for regeneration were obtained with IAA 17.13 μM and BA 8.9 μM. Formationof adventitious shoots via organogenesis from the petiole explant was verified by histological sectioning of plantlets. Regeneratedplants were transplanted into soil.
Plant Regeneration Via Organogenesis on Petiole of Centella asiatica (L.) Urban
Choi, Kyung-Mi,Hwang, Sung-Jin,Chung, Sang-Jin,Hwang, Baik The Korean Society of Medicinal Crop Science 2006 韓國藥用作物學會誌 Vol.14 No.2
An efficient plant regeneration of C. asiatica was achieved from organogenesis using petiole explants of in vitro plantlet on MS basal medium controled with different plant growth regulators (NAA,2,4-D, IAA kinetin, and BA). Best results that 50%, efficiency of regeneration per explant for regeneration were obtained with IAA $17.13\;{\mu}M$ and BA $8.9\;{\mu}M$. Formation of adventitious shoots via organogenesis from the petiole explant was verified by histological sectioning of plantlets. Regenerated plants were transplanted into soil.
Plant Regeneration Via Organogenesis on Petiole of Centella asiatica (L.) Urban
Kyung-Mi Choi,Sung-Jin Hwang,Sang-Jin Chung,Baik Hwang 韓國藥用作物學會 2006 한국약용작물학회지 Vol.14 No.2
An efficient plant regeneration of C. asiatica was achieved from organogenesis using petiole explants of in vitro plantlet on MS basal medium controled with different plant growth regulators (NAA,2,4-D, IAA kinetin, and BA). Best results that 50%, efficiency of regeneration per explant for regeneration were obtained with IAA 17.13 μM and BA 8.9 μM. Formation of adventitious shoots via organogenesis from the petiole explant was verified by histological sectioning of plantlets. Regenerated plants were transplanted into soil.