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알지네이트가 가교된 소장점막하조직 스폰지의 생체적합성 평가
송인범 ( In Bum Song ),이민숙 ( Min Suk Lee ),김문석 ( Moon Suk Kim ),강길선 ( Gil Son Khang ),이해방 ( Hai Bang Lee ) 한국조직공학과 재생의학회 2006 조직공학과 재생의학 Vol.3 No.3
Porcine small intestine submucosa(SIS), mainly composed of collagen and glycosaminoglycan, has been widely used as a material for organ and tissue reconstruction without immuno-rejection responses. Chemicallycrosslinked SIS sponges were prepared and characterized for bio-interactive wound dressings and tissue engineered scaffolds. SIS powder were reacted in aqueous solution of 3% acetic acid and 0.1% pepsin for 48 hrs and the prepared SIS solution was poured into mold and fabricated by freeze-drying method. The crosslinking reaction was performed using 1-ethyl-(3-3-dimethyl aminopropyl) carbodiimide hydrochloride(EDC) solution(distilled water(D.W.) : ethanol = 5 : 95) of 50, 100 and 200 mM concentration for 24 hrs. Then 1 wt% alginate solution was pass through the SIS sponge and lyophilzed. 102 mM calcium chloride(CaCl2) was used as crosslinking agent. The prepared sponges were characterized by scanning electron microscopy(SEM). The biocompatibility of NIH/3T3 fibroblast with these SIS sponges was found to be acceptable at a cell density of 2×104 cell/cm2. We investigated biodegradation of SIS sponge, MTT assay and H&E stain. From the MTT assay results, the biocompatibility of the crosslinked SIS sponge with NIH/3T3 fibroblast was confirmed. It was confirmed that SIS consists of organic components like tissue and showed inter-connective pores for adhesion and growth of cell. As a result of this study, we suggest that crosslinked alginate-SIS sponges fulfil many critical elements desirable in the application of wound dressing and tissue regeneration material.
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