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Bai, Yujia,Huang, Wenshu,Tao, Yongxia,Feng, Zuoshan The Korean Society for Applied Biological Chemistr 2014 Applied Biological Chemistry (Appl Biol Chem) Vol.57 No.1
The precise mechanism by which asafoetida extract influenced the growth of Pleurotus ferulae mycelium was investigated using a comparative proteomic analysis approach. Two types of asafoetida extracts were added to the medium of P. ferulae, and the effects of the extract on microstructure of P. ferulae mycelium were observed. Compared to the control group, the two asafoetida extracts played a significant role in promoting the growth of P. ferulae. Two-dimensional electrophoresis and LC-MS/MS were performed to examine changes in the expression of proteins in the mycelium of P. ferulae. A total of eight differentially expressed proteins were identified, including a heat shock protein, a flavin-containing monooxygenase, and a NADPH: quinone oxidoreductase type IV, as well as hypothetical and unknown proteins. Expression change of these proteins confirmed that asafoetida extracts significantly affected P. ferulae growth, metabolism, and secondary product metabolism. These results provide new insights into biochemical mechanisms underlying the relationship between P. ferulae and asafoetida.
Yujia Bai,Wenshu Huang,Yongxia Tao,Zuoshan Feng 한국응용생명화학회 2014 Applied Biological Chemistry (Appl Biol Chem) Vol.57 No.1
The precise mechanism by which asafoetida extractinfluenced the growth of Pleurotus ferulae mycelium wasinvestigated using a comparative proteomic analysis approach. Two types of asafoetida extracts were added to the medium of P. ferulae, and the effects of the extract on microstructure of P. ferulae mycelium were observed. Compared to the control group,the two asafoetida extracts played a significant role in promotingthe growth of P. ferulae. Two-dimensional electrophoresis andLC-MS/MS were performed to examine changes in the expressionof proteins in the mycelium of P. ferulae. A total of eightdifferentially expressed proteins were identified, including a heatshock protein, a flavin-containing monooxygenase, and a NADPH:quinone oxidoreductase type IV, as well as hypothetical andunknown proteins. Expression change of these proteins confirmedthat asafoetida extracts significantly affected P. ferulae growth,metabolism, and secondary product metabolism. These resultsprovide new insights into biochemical mechanisms underlying therelationship between P. ferulae and asafoetida.