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Safrolechitosan과 Eugenolchitosan의 항균효과
김제중 ( Je Jung Kim ),유희중 ( Hee Jung Ryoo ),정병옥 ( Byung Ok Jung ),박동기 ( Dong Ki Park ) 한국키틴키토산학회 2003 한국키틴키토산학회지 Vol.8 No.1
향균효과가 있는 것으로 알려진 천연물인 safrole과 eugenol을 단량체로하여 키토산과 그라프트공중합하여 키토산 유도체를 합성하여 향균활성저해효과를 측정한 결과 다음과 같은 결론을 얻었다. 키토산과 키토산 유도체의 점성도평균분자량은 키토산은 1.2×107, 키토산 유도체인 SaCs와 EuCs는 7.5×107과7.8×107 이었으며, FT?IR을 이용하여 키토산 및 키토산 유도체가 제조되었음을 확인하였다. 돌연변이 원성과 유해성 중금속은 모두 음성임을 확인할 수 있었으며, 구강미생물, 무좀균, 피부미생물, 식중독 세균 및 대장균에 대한 향균활성 저해효과는 모든 균이 키토산과 키토산 유도체의 점성도평균분자량이 클수록 우수한 향균활성저해효과를 나타냈으며, 투입농도에 따른 향균활성저해효과는 투입농도가 0.5% 일 때 모든 균에 대한 가장 좋은 향균활성저해효과를 나타내었다. 키토산유도체의 경우 실험에 사용된 모든 균에 대한 우수한 향균활성효과를 나타냄으로서 그 활용이 기대된다. The purpose of this research is to modify chitosan chemically using safrole and eugenol that are known for having good anitmicrobial for heightening its antimicrobial activites. After treating chitosan with safrole and eugenol we tested the chitosan for anitmicrobial. As the results of the test, EuCs showed 42-45%, 63-64% antimicrobial activites respectively when mouth microbial and foot care microbial was treated by diluted 0.5% EuCs. EuCs and Sacs showed 94.60% and 85.14% antimicrobial activites respectively when skin microbial was treated by 0.5% EuCs and Sacs. antimicrobial activites 87-97% and 60-97% when food poisoning microbial was treated by 0.5% EuCs and Sacs. Finally EuCs and Sacs showed pretty good antimicrobial activites 83% and 75% when pathogecnic E. coli was treated by 0.5% EuCs and Sacs. Mostly EuCs and Sacs showed much better antimicrobial activites than untreated chitosan did 6-22%
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C.J. González-Pérez1,I. Vargas-Arispuro,E. Aispuro-Hernández,C.L. Aguilar-Gil,Y.E. Aguirre-Guzmán,A. Castillo,A. Hernández-Mendoza,J.F. Ayala-Zavala,M.A. Martínez-Téllez1 한국미생물·생명공학회 2019 한국미생물·생명공학회지 Vol.47 No.2
The consumption of fresh vegetables has been related to recurrent outbreaks of foodborne diseases (FBD) worldwide. Therefore, the development of effective alternative technologies is necessary to improve the safety of these products. This study aimed to isolate and identify epiphytic lactic acid bacteria (LAB) from fresh fruits and leafy vegetables and characterize their antagonistic capacity due to their ability to produce bacteriocins or antibacterial compounds. For this, 92 LAB isolates from fruits and leafy vegetables were screened for antagonistic activity. Two strains with the highest and broadest antagonistic activities were selected for further characterization; one from cantaloupe melon (strain CM175) and one from cilantro leaves (strain C15). The cell-free supernatants (CFS) of CM175 and C15 were found to exhibit antagonistic activity against FBD-causing pathogens. The CM175 and C15 strains were identified as Pediococcus pentosaceus and Lactobacillus graminis, respectively. Notably, the P. pentosaceus CM175 CFS stopped the growth of Salmonella Typhimurium, Salmonella Saintpaul, Staphylococcus aureus, and Listeria monocytogenes, and delayed Escherichia coli O157:H7 growth. Moreover, L. graminis C15 CFS delayed the growth of all indicator pathogens, but did not completely stop it. Organic acids and bacteriocin-like molecules were determined to be possibly exerting the observed antagonistic activity of the identified LAB strains. Thus, application of the antagonistic compounds produced by Pediococcus pentosaceus and Lactobacillus graminis could be a novel and ecological strategy in developing antimicrobial biopreservatives for the food industry and mitigating FBD by reducing the biological contamination in fruit and vegetable orchards, mainly via their potential in controlling both gram-negative and gram-positive pathogenic bacteria.
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