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RISS 인기검색어
- 검색키워드
- 주제어 : Total internal reflection scattering microscopy (검색결과 2 건)
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Lee, Seungah,Park, Guenyoung,Chakkarapani, Suresh Kumar,Kang, Seong Ho Elsevier 2015 Biosensors & bioelectronics Vol.63 No.-
<P><B>Abstract</B></P> <P>Novel, fluorescence-free detection of biomolecules on nanobiochips was investigated based on plasmonic nanometal scattering in the evanescent field layer (EFL) using total internal reflection scattering (TIRS) microscopy. The plasmonic scattering of nanometals bonded to biomolecules was observed at different wavelengths by an electromagnetic field in the EFL. The changes in the scattering of nanometals on the gold-nanopatterned chip in response to the immunoreaction between silver nanoparticles and antibodies allowed fluorescence-free detection of biomolecules on the nanobiochips. Under optimized conditions, the TIRS immunoassay chip detected different amounts of immobilized antigen, i.e., human cardiac troponin I. The sandwich immuno-reaction was quantitatively analyzed in the dynamic range of 720zM–167fM. The limit of detection (<I>S</I>/<I>N</I>=4) was 600zM, which was ~140 times lower than limits obtained by previous total internal reflection fluorescence and dark field methods. These results demonstrate the possibility for a fluorescence-free biochip nanoimmunoassay based on the scattering of nanometals in the EFL.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Fluorescence-free detection of biomolecules on nanobiochips by total internal reflection scattering microscopy. </LI> <LI> Observation of the scattering shape and intensity of native nanometals by controlling optical components. </LI> <LI> Highly sensitive fluorescent-free detection of cTnI with a detection limit of 600zM. </LI> </UL> </P>
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Ahn, Sujin,Yu, Hyunung,Kang, Seong Ho Elsevier 2017 Biosensors & bioelectronics Vol.96 No.-
<P><B>Abstract</B></P> <P>Carcinoembryonic antigen (CEA) is a glycoprotein associated with colorectal carcinomas and is commonly used as a clinical tumor marker. Enhanced detection sensitivity for the assay of CEA molecules was achieved on a plasmonic nanoimmunosensor by wavelength-dependent transmission grating (TG)-based total internal reflection scattering microscopy (TIRSM). The plasmonic nanoparticles were placed in an evanescent field layer on a glass nanoimmunosensor that produced evanescent wave scattering by the total internal reflection of light from two lasers. The light scattered by target protein (CEA)-bound 20-nm silver nanoparticles (plasmonic nanoprobes) was collected and spectrally isolated in first-order spectral images (<I>n</I>=+1) by a TG (70 grooves/mm). The combination of evanescent wave scattering and TG significantly enhanced the detection sensitivity and selectivity due to the minimized spectroscopic interference and background noise. The TG-TIRSM method detected the CEA molecules at concentrations down to 19.75zM with a wide linear dynamic range of 19.75zM-39.50nM (correlation coefficient, <I>R</I>=0.9903), which was 45 to 1.25×10<SUP>9</SUP> times lower than the detection limits and 2×10<SUP>5</SUP> to 2×10<SUP>11</SUP> times wider than the dynamic ranges of previous assay methods. In particular, by simply changing the antibody of the target molecule, this technique can be used to detect various disease-related protein biomarkers directly in human biological samples at the single-molecule level.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Enhanced the detection sensitivity of CEA on nanoimmunosensor. </LI> <LI> Combined effects of wavelength-dependent TG-based TIRSM. </LI> <LI> Minimized the spectroscopic interference and background noise. </LI> <LI> 45 to 1.25×10<SUP>9</SUP> times lower than previous detection limits. </LI> <LI> 2×10<SUP>5</SUP> to 2×10<SUP>11</SUP> times wider dynamic range than previous methods. </LI> </UL> </P>
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