<P><B>Abstract</B></P> <P>Novel, fluorescence-free detection of biomolecules on nanobiochips was investigated based on plasmonic nanometal scattering in the evanescent field layer (EFL) using total internal reflection sc...
http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
https://www.riss.kr/link?id=A107636610
2015
-
SCI,SCIE,SCOPUS
학술저널
444-449(6쪽)
0
상세조회0
다운로드다국어 초록 (Multilingual Abstract)
<P><B>Abstract</B></P> <P>Novel, fluorescence-free detection of biomolecules on nanobiochips was investigated based on plasmonic nanometal scattering in the evanescent field layer (EFL) using total internal reflection sc...
<P><B>Abstract</B></P> <P>Novel, fluorescence-free detection of biomolecules on nanobiochips was investigated based on plasmonic nanometal scattering in the evanescent field layer (EFL) using total internal reflection scattering (TIRS) microscopy. The plasmonic scattering of nanometals bonded to biomolecules was observed at different wavelengths by an electromagnetic field in the EFL. The changes in the scattering of nanometals on the gold-nanopatterned chip in response to the immunoreaction between silver nanoparticles and antibodies allowed fluorescence-free detection of biomolecules on the nanobiochips. Under optimized conditions, the TIRS immunoassay chip detected different amounts of immobilized antigen, i.e., human cardiac troponin I. The sandwich immuno-reaction was quantitatively analyzed in the dynamic range of 720zM–167fM. The limit of detection (<I>S</I>/<I>N</I>=4) was 600zM, which was ~140 times lower than limits obtained by previous total internal reflection fluorescence and dark field methods. These results demonstrate the possibility for a fluorescence-free biochip nanoimmunoassay based on the scattering of nanometals in the EFL.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Fluorescence-free detection of biomolecules on nanobiochips by total internal reflection scattering microscopy. </LI> <LI> Observation of the scattering shape and intensity of native nanometals by controlling optical components. </LI> <LI> Highly sensitive fluorescent-free detection of cTnI with a detection limit of 600zM. </LI> </UL> </P>
One-dimensional nanostructures based bio-detection
3D label-free prostate specific antigen (PSA) immunosensor based on graphene–gold composites