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        Optimization of the microemulsion formulation of curcuma oil and evaluation of its acaricidal efficacy against Tetranychus cinnabarinus (Boisduval) (Acari: Tetranychidae)

        Cheng Zuo-Hui,Fan Fang-Fang,Zhao Jin-Zhong,Li Rui,Li Sheng-Cai,Zhang En-Jia,Liu Yu-Kun,Wang Jue-Ying,Zhu Xiang-Run,Tian Yong-Ming 한국응용곤충학회 2020 Journal of Asia-Pacific Entomology Vol.23 No.4

        The microemulsion formulation (hereafter formulation) of curcuma oil and its acaricidal efficacy against Tetranychus cinnabarinus Boisduval (Acari: Tetranychidae) were optimized in the laboratory to evaluate their spray effectiveness of oviposition inhibition and repellence. Ethovision XT6 was used to analyse the effects of the sublethal concentrations (LC 20 ) of curcuma oil and the formulation on the behaviors of T. cinnabarinus. The results showed that Tween-80 was the best surfactant, Isopropanol was the best co-surfactant and K m = 2:1 was the best condition for the formulation. The prepared microemulsions are stable under conditions of centrifugation and incubation for extended periods. The results showed that the effect of the spray bioassays of the formulation against T. cinnabarinus continuously increased during the experiment, but for curcuma oil almost no longer increase observed when the exposure time went beyond 24 h. Moreover, compared with curcuma oil (LC 50 = 0.716%), the spray bioassay of the formulation (LC 50 = 0.035%) was stronger against T. cinnabarinus. The repellency of the formulation to T. cinnabarinus was stronger with increasing exposure time, but that of curcuma oil declined after 12 h of exposure. The mobile distance of T. cinnabarinus treated with the formulation continuously declined during the experiment but that due to the curcuma oil almost no longer declined when the treatment time reached 12 h. The maximum mobile frequency of T. cinnabarinus treated by curcuma oil and the formulation was recorded at 6 h and 12 h, respectively. Thus, the formulation is a promising candidate as a botanical acaricide of green vegetables.

      • KCI등재

        Cloning and different expression of ATP synthase genes between propargite resistant and susceptible strains of Tetranychus cinnabarinus (Acarina: Tetranychidae)

        Shu-Jun Wei,Jing Ni,Kuanyu Zheng,Zhenguo Yang,Daoyan Xie,Aisi Da,Jianping Chai,Xiujun Jiang,Shaoxiang Li 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.1

        The carmine spider mite, Tetranychus cinnabarinus (Boisduval), is a serious phytophagous mite damaging importantcrops and can rapidly develop resistance to acaricides. Mitochondrial ATP synthase (F1F0 ATP synthase)is an important target site of acaricides. The role of ATP synthase in acaricide resistance remains unclear at themolecular level. In this study, twelve full-length cDNAs of ATP synthase genes were cloned and characterizedfrom T. cinnabarinus and their expression levels were determined for both progargite-resistant and susceptiblestrains. The effect of propargite exposure on gene expression was also evaluated. Analyses of gene expressionrevealed that TcATPsynU-2, TcATPsynF0-2 and TcATPsynF0-4 were significantly down-regulated in the progargite-resistant strain. TcATPsynF0-2 and TcATPsynF0-4 had a strong response to progargite exposure. Theresults suggest that lower levels of TcATPsynU-2, TcATPsynF0-2 and TcATPsynF0-4 expression might be related topropargite-resistance observed in the resistant T. cinnabarinus. This is the first attempt to identify specific ATPasegenes involved in propargite resistance in T. cinnabarinus.

      • KCI등재

        Acaricidal activity of Aloe vera L. leaf extracts against Tetranychus cinnabarinus (Boisduval) (Acarina: Tetranychidae)

        Jie Wei,Wei Ding,Yan-Guo Zhao,Patcharaporn Vanichpakorn 한국응용곤충학회 2011 Journal of Asia-Pacific Entomology Vol.14 No.3

        Four different extracts of Aloe vera L. leaves were evaluated for acaricidal activity against female adults of carmine spider mite, Tetranychus cinnabarinus (Boisduval), by slide-dip bioassay. At 72 h after treatment, the acetone extract showed the strongest acaricidal activity with LC_50 value of 90 ppm. The LC_50 values for ethyl acetate, water, and ethanol extracts were 113, 340, and 391 ppm, respectively. The acetone extract was fractionated using a silica gel column. Among the twenty-two fractions obtained the fifth, tenth, eleventh,twelfth, fifteenth, and seventeenth fractions showed strong acaricidal activity, causing 80.39 to 92.16%mortality at 72 h after treatment. The tenth and eleventh fractions had the strong activity, with LC_50 values of 44 ppm and 33 ppm, respectively. The results suggested that A. vera has a great potential for development as a botanical acaricide for T. cinnabarinus control.

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