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The Effect of Cultural Factors on Anther Culture in Spring Wheat (Triticum aestivum)
Kang,Tae-Jin,Yang,Deok-Chun 한국자원식물학회 2003 Plant Resources Vol.6 No.2
Effects of shaking, medium consistency and anther density on polyhaploid production in two wheat cultivars, Pavon and Chris, were studied using a modified 85D12 medium. Pavon produced more calli in shaking and more albino plants tban Chris. However, Chris produced threefold more green plants than Pavon in non-shaking treatment. More calli and green plants were derived from non-shaking treatment than those from shaking treatment. Anthers were cultured on both liquid and semi-solid 85D12 media, using two anther densities, 48 and 96 anthers per plate. Although Pavon generally produced more calli and albino plants than Chris, Chris produced more green plants than Pavon. More green plants were derived from semi-solid medium than those from liquid medium. A factor that may affect plant regeneration from anthers is the length of time on initiation medium. Most of the calli for both genotypes were transferred during the first two time periods. Fertility, as measured by seed set, was determined for all surviving regenerated plants. About 24% of Chris and Pavon anther-derived green plants in the experiment of medium consistency and anther density produced seed.
( Dong Sup Jung ) 전북대학교 인문학연구소 2014 건지인문학 Vol.12 No.-
Las hermanas coloradas, the Eugenio Nadal prizewinning novel in 1969,maintains the form of the detective or criminal novel with the protagonist Plinio, the chief of spanish local police of Tomelloso. Nevertheless, this workis not merely a simple detective or criminal novel where a hero resolves the criminal problem. Las hermanas coloradas contains more significances than we expect from a simple detective novel. This paper investigates the localism and the criticism of Garcia Pavon``s major work. Because this novel not only expresses the reality of rural and urban Spain, but also criticizes it with humor and affection. The trick and interest of the criminal novel exist more than in other works. And, as the other works of the writer, the liricism and the emotion based on the refined language dominate the work. So Las hermanas coloradas is a kind of open work.