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      • SCIESCOPUSKCI등재

        Distribution and activity of sulfate-reducing bacteria in lake soyang sediments

        Jin, Hoo-Yong,Lee, Dong-Hun,Zo, Young-Gun,Kang, Chan-Su,Kim, Sang-Jong The Microbiological Society of Korea 1996 The journal of microbiology Vol.34 No.2

        In order to known the extend of contribution to the degradation of organic materials and nutrient recycling by sulfate-reducing bacteria (SRB) and methane-producing bacteria (MPB) in sediment, the distribution and activity of these two groups of microorganisms were studied montly in 1994 at two sites, one littoral (Sanggulri) and the other profunndal (DAM), in Lake Soyang. In the seasonal distribution of two microorganisms, SRB were 1.07 $\times$ 10$^{3}$-2.42 $\times$ 10$^{5}$ cells/g-dry weight at Sanggulri, 2.40 $\times$ 10$^{5}$ -1.29 $\times$ 10$^{6}$ at Dam and MPB were 0.52 $\times$ 10$^{3}$ cells/g-dry weight at Sangguri and 1.44 $\times$ 10$^{3}$-6.89 $\times$ 10$^{3}$ at Dam. In these results, the density of SRB in Lake Soyang is much higher than other lakes. These high values might be due to higher sulfate concentration, 0.69-4.05 mM, than normal freshwater, 0.01-1.2 mM. And a good correlation of SRB and chlorophyll a concentration implied that the important environmental factor on distribution of SRB might be the concentration of available organic matter. In a comparison of sulfate-reducing rate and methane producing rate in 1995, the activity of SRB for the degradation of organic matter was higher than MPB by factor of 359. Conclusively SRB superior to MPB in the distribution and activity are more important annearobic bacteria in Lake Soyang sediments.

      • KCI등재후보

        슬러리형 돈사폐수의 혐기성 소화시 암모니아 부하의 영향

        원철희,권재혁,임재명 유기성자원학회 2009 유기물자원화 Vol.17 No.1

        This research examined the effect of ammonia nitrogen loading rate(NVLR) on the anaerobic digestion of slurry-typed swine wastewater. The anaerobic reactor was used an upflow anaerobic sludge blanket (UASB) process. This UASB reactor was operated at a NVLR of 0.02~0.96kgNH4+-N/㎥/day. The methane content showed the range of 73.3~77.9% during the steady state period. Free ammonia(FA) concentration increased over inhibition level as pH increase from 7.3 to 8.2. However, in consideration of methane content, methane producing bacteria (MPB) inhibition by FA and total ammonia(TA) was not observed. A stepwise increase of the NVLR resulted in a deterioration in the COD removal rate in UASB reactor. The COD removal rate were 60% for NVLR up to 0.55kgNH4+-N/㎥/day. As the NVLR increased from 0.09 to 0.96kgNH4+-N/㎥/day, the biogas production rate varied from 3.71 to 9.14L/d and the methane conversion rate of the COD varied from 0.32 to 0.20㎥CH4/kg COD removed. Consequently, in considerations of FA concentration, COD removal rate, and CH4 production rate, the UASB reactor must be operated to lower than 0.40kgNH4+-N/㎥/day of NVLR.. 본 연구에서는 UASB 반응조를 이용한 슬러리형 돈사폐수의 혐기성 소화시 암모니아 부하의 영향을 평가하였다. UASB 반응조는 0.02~0.96kgNH4+-N/㎥/day 범위의 NVLR로 운전되었으며, 정상상태에서 biogas내 메탄함량은 73.3~77.9%였다. FA 농도는 메탄생성미생물의 저해 범위까지 증가하였으나, 메탄함량을 고려할 때, FA와 TA로 인한 저해는 발생하지 않았다. NVLR이 증가함에 따라 COD 제거율은 악화되었으며, 0.55kgNH4+-N/㎥/day 이하의 부하에서 COD 제거율을 60% 이상으로 유지할 수 있었다. NVLR이 0.09에서 0.96kgNH4+-N/㎥/day로 증가함에 따라, biogas의 생성량은 3.71에서 9.14L/day로 증가하였으며, COD의 메탄으로 전환율은 0.32에서 0.20㎥CH4/kg으로 감소하였다. FA농도, COD 제거율, 메탄생성률 등을 고려할 때, UASB 반응조는 0.40kgNH4+-N/㎥/day 이하의 NVLR로 운영되어야 한다.

      • KCI등재후보

        상향류 혐기성 블랭킷 반응조를 이용한 프로피온산의 혐기성 처리시 고농도 황산염의 영향

        이채영 유기성자원학회 2008 유기물자원화 Vol.16 No.3

        Two UASB reactors were operated to investigate the effect of high concentration of sulfate on anaerobic digestion of propionate using an upflow anaerobic sludge blanket(UASB) reactor. An organic loading rate of 1.2 kg COD/m3·d and a hydraulic retention time of 1.6 d were maintained during this study. In the absence of sulfate, the UASB reactor achieved about 95% removal of chemical oxygen demand whereas in the presence of 2,000 SO4 2-mg/L, the COD removal rate decreased to 83% due probably to the inhibition of dissolved sulfide inhibition. Interactions between the methane producing bacteria (MPB) and sulfate reducing bacteria (SRB) were measured to investigate the competition between MPB and SRB. The MPB consumed average 58% of the available electron donors at COD/SO4 2- ratio of 1. Propionate was consumed mainly by SRB, converting sulfate into sulfide and suppressing the methane production. The specific methanogenic activity (SMA) using acetate and propionate increased as microorganism acclimated to the substrate.

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