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      • 한국인 정상안 맥락막의 일중변동

        San Seong,Yang Jae Kim,Ju Young Kim,Jin Hae Lee,Hyun Sup Oh,Soon Hyun Kim,Oh Woong Kwon,Yong Sung You 한국망막학회 2018 Journal of Retina Vol.3 No.2

        Purpose: To determine the diurnal variation in choroidal thickness and choriocapillaris flow measured by optical coherence tomography (OCT) and optical coherence tomography angiography (OCTA) Methods: This study was a prospective study of 15 normal, young volunteers (30 eyes) who underwent OCT and OCTA examination 5 times a day at 2-hour intervals. Choroidal and choriocapillaris thicknesses were measured with the enhanced depth imaging mode of OCT. The area of flow was measured in 1-, 3-, and 6-mm-diameter areas centered at the fovea and analyzed with the split-spectrum amplitude- decorrelation angiography algorithm of OCTA. Choroidal and choriocapillaris thicknesses and flows were analyzed independently by two vitreoretinal specialists. Results: Choroidal thickness showed a significant diurnal variation. The maximum thickness was 311.31 μm at 9 a.m., and the minimum thickness was 266.75 μm at 5 p.m. (p < 0.001). In contrast, the mean choriocapillaris thickness was 15.14 μm, and remained relatively constant without a significant diurnal variation (p = 0.694). The mean choriocapillaris flow area was 0.45 mm2 (p = 0.238) in a 1 mm area, 4.22 mm2 (p = 0.230) in a 3-mm diameter area, and 16.94 mm2 (p = 0.062) in a 6-mm diameter area. No diurnal variation was observed. Conclusions: Despite significant diurnal variation in choroidal thickness, choriocapillaris thickness and flow remained relatively constant over time.

      • KCI등재

        Flow Void Analysis Using Different Thresholding Methods on a Choriocapillaris Optical Coherence Tomography Angiography Image Complemented with a Structural En Face Image

        이영주,Soon-Young Hwang,Cheol Min Yun 대한안과학회 2024 Korean Journal of Ophthalmology Vol.38 No.1

        Purpose: To investigate the flow characteristics using different thresholding methods on a choriocapillaris optical coherence tomography angiography (OCTA) image complemented with a structural en face image. Methods: The 42 choriocapillaris OCTA images from healthy subjects were obtained with swept-source OCTA device and the 3 × 3-mm area OCTA images were processed with ImageJ. Using a raw choriocapillaris OCTA image and structural en face image, we adjusted the different structural signal intensity. Then, the raw images and adjusted images were binarized with a global threshold and an auto local threshold using the Phansalkar method at 1- or 2-intercapillary distance. Then, the mean area, number, and size of the flow void, were compared among the images using different thresholding methods. Results: Mean flow void area, number, and size were different according to the different binarization method both in raw and adjusted images (all p < 0.001). The mean flow void area analyzed with global threshold method were well correlated with those with auto local threshold method both in raw and adjusted images (all intraclass correlations, >0.929). Conclusions: Flow void features varied according to the different binarization methods but showed good correlation. The flow void characteristics according to the different binarization methods should be considered for the analysis of the choriocapillaris OCTA images complemented with a structural en face image.

      • KCI등재후보

        Retinal and Choroidal Vasculature Before and After Patch Occlusion Treatment Using Optical Coherence Tomography Angiography in Patients with Amblyopia

        김재곤,이세엽,이동철 계명대학교 의과대학 2023 계명의대학술지 Vol.42 No.1

        This study aimed to investigate structural differences in the retinal and choroidal blood vessels before and after patch occlusion treatment in patients with amblyopia using optical coherence tomography angiography (OCTA) and to determine whether these differences are related to clinical improvement in patients. A total of 26 eyes of 17 patients with monocular or binocular amblyopia who underwent patch occlusion treatment for at least 1 month were retrospectively enrolled. The width of the foveal avascular zone, retinal blood vessel density, choroidal thickness, and choroidal vascularity index (CVI) measured by OCTA were compared before and after the treatment; the correlation with change in best-corrected visual acuity (BCVA) was analyzed for the data showing statistically significant differences. The mean BCVA of amblyopic eyes before and after patch occlusion treatment was 0.41 ± 0.23 and 0.25 ± 0.16 in logarithm of the minimum angle of resolution units, respectively. A decrease of about 2% in CVI was observed after the treatment (<i>p</i> = 0.011). The correlation between the changes in CVI and the changes in BCVA were insignificant (Rs = 0.086, <i>p</i> = 0.718). The results indicated that a decrease in CVI was observed after the patch occlusion treatment, but the relationship between CVI and BCVA could not be established. Patch occlusion treatment in amblyopia appears to affect the changes in the choroidal vessels and stroma.

      • SCOPUSKCI등재

        Effect of Basic Fibroblast Growth Factor-Saporin (bFGF-SAP) Conjugate on Bovine Choriocapillary Endothelial Cells

        Yeong Hoon Kim,Hwa Seon Kim,Jin Seong Yoo 대한안과학회 2004 Korean Journal of Ophthalmology Vol.18 No.2

        We evaluated the effect of a basic fibroblast growth factor (bFGF) and saporin conjugate (bFGF-SAP) on proliferation, migration and tubule formation in bovine choriocapillary endothelial cells (BCECs). Cell proliferation and MTS assays were done with 0.01, 0.1, 1, 10, and 100 nM bFGF-SAP, and an equimolar concentration of bFGF and saporin. TUNEL assay was performed to confirm apoptosis. Cells were treated with 1, 10, and 100 nM bFGF-SAP and migration assay and tubule formation assay were done. Results were evaluated with image analysis. All experiments were performed in triplicate and repeated three times. Viable cells (ID50 = 0.62) and cell proliferation by MTS assay (ID50 = 0.75 nM) were inhibited. Saporin caused cytotoxicity and inhibition of proliferation at high concentration. DNA fragmentation was identified by TUNEL assay. Migration and tubule formation were also inhibited. All mechanisms responsible for neovascularization were inhibited, and this could be applied in the management of subretinal choroidal neovascularization (SRN).

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