http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Cephalosporin C 내성과 7-Aminocephalosporanic Acid 감수성을 지닌 균주의 선발 및 특성
김욱현,박용춘,임재윤,김영창 한국산업미생물학회 1995 한국미생물·생명공학회지 Vol.23 No.5
A strain which showed cephalosporin C resistance and 7-aminocephalosporanic acid sensitivity was isolated from nature. Among the isolates, SS5 was sensitive to cephalosporin C, penicillin G, ampicillin, 7-aminocephalosporanic acid, 6-aminopenicillanic acid, and 7-aminodeacetoxy cephalosporanic acid at concentrations of 1,000 ㎍/㎖, 2,000 ㎍/㎖, 3,000 ㎍/㎖, 30 ㎍/㎖ 100 ㎍/㎖ and 100 ㎍/㎖, respectively. But SS5 was sensitive at very how concentration of chloramphenicol, kanamycin, neomycin, streptomycin and tetracycline. Since SS5 was sensitive to 7-ACA (30 ㎍/㎖) and didn’t have β-lactamase activity on the cephalosporin C, SS5 could be useful as an indicator strain for the production of 7-ACA, which is an important precursor for the synthesis of many semisynthetic cephalosporins.
Cephalosporin C Acylase 생산균주의 분리 및 특성
박용춘,김욱현,임재윤,김영창 한국산업미생물학회 1995 한국미생물·생명공학회지 Vol.23 No.5
7-aminocephalosporanic acid(7-ACA)에 내성이 있으나 cephalosporin 계 항생제에는 민감한 Micrococcus luteus ATCC 9341을 지시균으로 사용하고, D-(α)-phenylglycine methylester와 7-ACA, 도는 glutaric acid dimethyl ester와 7-ACA를 기질로 사용한 bioassay 방법을 acylase 활성에 의하여 집락 주위에 지시균의 생장 저지환을 생성하는 20 종류의 세균을 선발하였다. 환 생성균들 중에서 cephalosporin C, glutaryl 7-ACA에는 내성이 있으나 7-ACA에는 감수성이 있는 SS5 균을 이용한 bioassay 방법과 HPLC 분석을 통하여 cephalosporin C acylase 활성이 있는 APS20 균주를 최종적으로 선발하였으며, 이 균주는 Bacillus macerans로 동정되었다. B. macerans APS20은 cephalosporin C에 대한 β-lactamase 활성이 없었으나, 100 ㎍/㎖ 농도의 cephalosporin C에 내성을 나타내었다. 그러나 penicillin G의 경우에는 50 ㎍/㎖에서도 생장하지 못하였다. Twenty microbial strains producing the acylase were isolated from soil by using Micrococcus luteus ATCC 9341 as an indicator strain, using either D-(α)-phenylglycine methylester and 7-aminocephalosporanic acid (7-ACA) or glutaric acid dimethylester and 7-ACA as substrates. Among the isolates, only one strain was turned out to be the 7-ACA producer from either cephalosporin C or glutaryl 7-ACA as the substrates by using the overlay of 7-ACA sensitive strain (SS5). 7-ACA produced from cephalosporin C by an isolate (APS20) was detected by high performance liquid chromatography. The isolated strain (APS20) was identified to Bacillus macerans on the basis of cellular fatty acid profile by gas chromatography. Bacillus macerans APS20 had no β-lactamase activity on cephalosporin C, and that is very important for the enzymatic production process of 7-ACA. However, this strain was resistant up to 100 ㎍/㎖ of cephalosporin C.
Jeong, Yoo-Seok,Yoo, Hyo-Jin,Kim, Sang-Dal,Nam, Doo-Hyun,Khang, Yong-Ho The Korean Society for Biotechnology and Bioengine 2005 Biotechnology and Bioprocess Engineering Vol.10 No.6
Pseudomonas cepacia BY21 was found to produce glutaryl acylase that is capable of deacylating glutaryl-7-aminocephalosporanic acid (glutaryl-7-ACA) to 7-aminocephalosporanic acid (7-ACA), which is a starting material for semi-synthetic cephalosporin antibiotics. Amino acids of the reported glutaryl acylases from various Pseudomonas sp. strains show a high similarity (>93% identity). Thus, with the known nucleotide sequences of Pseudomonas glutaryl acylases in GenBank, PCR primers were designed to clone a glutaryl acylase gene from P. cepacia BY21. The unknown -subunit gene of glutaryl acylase from chromosomal DNA of P. cepacia BY21 was cloned successfully by PCR. The -subunit amino acids of P. cepacia BY21 acylase (GenBank accession number AY948547) were similar to those of Pseudomonas diminuta KAC-1 acylase except that Asn408 of P. diuminuta KAC-1 acylase was changed to Leu408.
Kim, Dae-Weon,Kang, Sang-Mo,Yoon, Ki-Hong The Microbiological Society of Korea 1999 The journal of microbiology Vol.37 No.4
7-Aminocephalosporanic acid (7-ACA) is the initial compound in preparation of cephalosporin antibiotics widely used in clinical treatment. Bacteria producing glutaryl 7-ACA acylase, which convert cephalosporin C to 7-ACA, has been screened in soil samples. A bacterial strain exhibiting high glutaryl 7-ACA acylase activity, designated KAC-1, was isolated and identified as a strain of Pseudomonas diminuta by characterizing its morphological and physiological properties. The screening procedures include culturing on enrichment media containing glutaric acid, glutamate, and glutaryl 7-aminocephalosporanic acid as selective carbon sources. To enhance enzyme production, optimal cultivation conditions were investigated. This strain grew optimally at pH 7 to 9 and in temperatures of 20 to 40 C, but acylase production was higher when the strain was grown at 25 C. Glutaric acid, glutamate and glucos also acted as inducers for acylase production. In a jar fermenter culture, P. diminuta KAC-1 produce acylase in a growth-associated manner. The substrate specificity of KAC-1 acylase by cell extract showed that this enzyme had specificity toward glutaryl 7-ACA, glutaryl 7-ADCA, but not cephalosporin C.
Knang, Yong-Ho,Yoo, Ryong-Hoon The Korean Society for Biotechnology and Bioengine 1997 Biotechnology and Bioprocess Engineering Vol.2 No.2
A search was undertaken to screen microorganisms that produce an enzyme capable of deacylating glutary1-7-amincephalosporanic acid to 7-aminocephalosporanic acid in soil samples. The screening was carried out by preparing enrichment cultures containing glutary-7ACA and cephalosporin C as selective carbon sources. A non-${\beta}$-lactam model compound,, glutary-p-nitroanilide, was synthesized as a substrate suitable for the rapid screening of microorganisms isolated from the enrichment cultures. Two isolates exhibiting acylase activity, designated BY7.4 and BY8.1, were identified as strains of Pseudomonas species. Pseudomonas BY8.1 showed higher acylase activity toward G1-7ACA than Pseudomonas BY7.4. Environmental conditions for the optimal acylase activity of Pseudomonas BY8.1 were shown to be pH9 and 30$^{\circ}C$.