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Long Non-Coding RNA RMRP Contributes to Sepsis-Induced Acute Kidney Injury
Xia Zhang,Zhongwei Huang,Yan Wang,Ting Wang,Jingjing Li,Peipei Xi 연세대학교의과대학 2021 Yonsei medical journal Vol.62 No.3
Purpose: This study aimed to explore the role of the long non-coding RNA (lncRNA) RNA component of mitochondrial RNAase P (RMRP) in sepsis-induced acute kidney injury (AKI). Materials and Methods: Venous blood was collected from septic patients and healthy people. C57BL/6 mice who underwent cecal ligation and puncture (CLP) were used as in vivo models of septic AKI. Lipopolysaccharide (LPS)-induced HK-2 cells were employed as in vitro models of AKI. Flow cytometry analysis was conducted to detect cell apoptosis. Enzyme-linked immunosorbent assay and Western blot assays were used to detect levels of pro-inflammatory cytokines. Results: RMRP was upregulated in sera from patients with AKI and in LPS-induced cells. Knockdown of RMRP inhibited cell apoptosis and reduced production of inflammatory factors in LPS-induced cells, as well as alleviated AKI in CLP mice. RMRP facilitated inflammation by activating NACHT, LRR, and PYD domains-containing protein 3 (NLRP3) inflammasome. We found that microRNA 206 (miR-206) binds with and is negatively regulated by RMRP: miR-206 directly targets the 3’ untranslated region of DEAD-box helicase 5 (DDX5) and negatively regulates DDX5 expression. By binding with miR-206, RMRP upregulated DDX5 expression. Rescue assays revealed that overexpression of DDX5 counteracted the effect of RMRP inhibition on cell apoptosis and inflammatory response in LPS-induced cells. Conclusion: The lncRNA RMRP contributes to sepsis-induced AKI through upregulation of DDX5 in a miR-206 dependent manner and through activation of NLRP3 inflammasome. This novel discovery may provide a potential strategy for treating AKI.
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Ovarian transcriptomic analysis of Shan Ma ducks at peak and late stages of egg production
Zhu, ZhiMing,Miao, ZhongWei,Chen, HongPing,Xin, QingWu,Li, Li,Lin, RuLong,Huang, QinLou,Zheng, NenZhu Asian Australasian Association of Animal Productio 2017 Animal Bioscience Vol.30 No.9
Objective: To assess the differences in ovarian transcriptomes in Shan Ma ducks between their peak and late stages of egg production, and to obtain new transcriptomic data of these egg-producing ducks. Methods: The Illumina HiSeq 2000 system was used for high throughput sequencing of ovarian transcriptomes from Shan Ma ducks at their peak or late stages of egg production. Results: Greater than 93% of the sequencing data had a base quality score (Q score) that was not less than 20 (Q20). From ducks at their peak stage of egg production, 42,782,676 reads were obtained, with 4,307,499,083 bp sequenced. From ducks at their late stage of egg production, 45,316,166 reads were obtained, with 4,562,063,363 bp sequenced. A comparison of the two datasets identified 2,002 differentially expressed genes, with 790 upregulated and 1,212 downregulated. Further analysis showed that 1,645 of the 2,002 differentially expressed genes were annotated in the non-redundant (NR) database, with 646 upregulated and 999 downregulated. Among the differentially expressed genes with annotations in the NR database, 696 genes were functionally annotated in the clusters of orthologous groups of proteins database, involving 25 functional categories. One thousand two hundred four of the differentially expressed genes with annotations in the NR database were functionally annotated in the gene ontology (GO) database, and could be divided into three domains and 56 categories. The three domains were cellular component, molecular function, and biological process. Among the genes identified in the GO database, 451 are involved in development and reproduction. Analysis of the differentially expressed genes with annotations in the NR database against the Kyoto encyclopedia of genes and genomes database revealed that 446 of the genes could be assigned to 175 metabolic pathways, of which the peroxisome proliferator-activated receptor signaling pathway, insulin signaling pathway, fructose and mannose metabolic pathways, gonadotropin releasing hormone signaling pathway and transforming growth factor beta signaling pathway were significantly enriched. Conclusion: The differences in ovarian transcriptomes in Shan Ma ducks between their peak and late stages of egg production were elucidated, which greatly enriched the ovarian transcriptomic information of egg-producing ducks.
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Simulation of the hydraulic isolation efficiency during multistage hydrajet fracturing
Xin Fan,Gensheng Li,Zhongwei Huang,Shouceng Tian,Xianzhi Song,Chen Yang 한국자원공학회 2015 Geosystem engineering Vol.18 No.5
Effective isolation between target zones is critical to multistage fracturing. Low pressure zone caused by the hydrajet is used to isolate stages in the multistage hydrajet fracturing technology. However, there are few researches available in the literature that investigates the hydraulic isolation efficiency considering the existence of multiple fractures. This paper builds a three-dimensional computational-fluid-dynamics model with FLUENT software to study the flow field along annular sections under different conditions. It is verified that the results obtained from the model are consistent with experimental data along the perforation tunnel. The mass flow rate ratio between two outlets is proposed to analyze the hydraulic isolation efficiency in hydrajet multistage fracturing. The results show that annular fluid converges into the perforation tunnel due to the high-speed hydrajet even though there is a pre-existing fracture behind. Effective hydraulic isolation can be generated by reducing the pressure differences between target zones and the annular injection velocity as well as by enlarging the jet velocity and the area ratio. It is found that there exists an optimum area ratio to get the maximum isolation efficiency. Sensitivity analysis indicates the pressure difference is the dominant factor affecting the hydraulic isolation efficiency. A fully quadratic model derived from the response surface method is presented to predict the hydraulic isolation efficiency for real fracturing. This study will be able to provide practical guidance for the optimization of hydrajet multistage fracturing.
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Li Hua,Guo Junyu,Qin Zhongwei,Wei Mingwei,Guo Houji,Huang Fuda 대한독성 유전단백체 학회 2023 Molecular & cellular toxicology Vol.19 No.3
Background Emerging evidence has indicated that circular RNAs (circRNAs) play vital roles in colorectal cancer (CRC). CircRNA leucine zipper and EF-hand containing transmembrane protein 1 (circLETM1) have been found to be ectopic in CRC, but their role in CRC has not been studied yet. Objective This study aims to explore the roles of circLETM1 in CRC and the possible molecular mechanism. Result The results showed that circLETM1 was highly expressed in CRC cells, and was localized at the cytoplasm. Functional assays verifi ed that overexpression of circLETM1 could facilitate, but circLETM1 downregulation could hamper proliferation, migration and invasion abilities of CRC cells. In addition, mechanism assays found that circLETM1 absorbed and reduced microRNA (miR)-143-3p expression to promote keratin 80 (KRT80). Rescue experiments proved that circLETM1 mediated CRC proliferation, migration and invasion through KRT80. Conclusion Taken together, our results suggested that circLETM1 promotes proliferation, migration and invasion through the miR-143-3p/KRT80 axis in CRC. Therefore, targeting circLETM1 may provide a novel option for CRC treatment.
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