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DNS of vortex-induced vibrations of a yawed flexible cylinder near a plane boundary
Zhimeng Zhang,Chunning Ji,Md. Mahbub Alam,Dong Xu 한국풍공학회 2020 Wind and Structures, An International Journal (WAS Vol.30 No.5
Vortex-induced vibrations of a yawed flexible cylinder near a plane boundary are numerically investigated at a Reynolds number Ren = 500 based on normal component of freestream velocity. Free to oscillate in the in-line and cross-flow directions, the cylinder with an aspect ratio of 25 is pinned-pinned at both ends at a fixed wall-cylinder gap ratio G/D = 0.8, where D is the cylinder diameter. The cylinder yaw angle () is varied from 0 to 60 with an increment of 15. The main focus is given on the influence of on structural vibrations, flow patterns, hydrodynamic forces, and IP (Independence Principle) validity. The vortex shedding pattern, contingent on , is parallel at =0 , negatively-yawed at 15 and positively-yawed at 30. In the negatively- and positively-yawed vortex shedding patterns, the inclination direction of the spanwise vortex rows is in the opposite and same directions of , respectively. Both in-line and cross-flow vibration amplitudes are symmetric to the midspan, regardless of . The RMS lift coefficient CL,rms exhibits asymmetry along the span when 0, maximum CL,rms occurring on the lower and upper halves of the cylinder for negatively- and positively-yawed vortex shedding patterns, respectively. The IP is well followed in predicting the vibration amplitudes and drag forces for α ≤ 45° while invalid in predicting lift forces for α ≥ 30°. The vortex-shedding frequency and the vibration frequency are well predicted for = 0 - 60 examined.
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Jinyong Zhang,Muchun He,Zilong Xiang,Shufang Liu,ZhiMeng Zhuang 한국유전학회 2019 Genes & Genomics Vol.41 No.6
Background The subpeduncle lobe/olfactory lobe–optic gland axis is called the endocrine regulation center of cephalopods. However, little is known about the mechanism of the subpeduncle lobe/olfactory lobe-optic gland axis regulate the sexual maturation and post-reproductive death of Sepia esculenta Hoyle. Objectives The primary objective of this study was to provide basic information for revealing the mechanism of the subpeduncle lobe/olfactory lobe–optic axis regulating the rapid post-reproductive death of S. esculenta. Methods In this paper, Illumina sequencing based transcriptome analysis was performed on the brain tissue of female S. esculenta in the three key developmental stages: growth stage (BG), spawning stage (BS), and post-reproductive death stage (BA). Results A total of 66.19 Gb Illumina sequencing data were obtained. A comparative analysis of the three stages showed 2609, 3333, and 170 differentially expressed genes (DEGs) in BG-vs-BA, BG-vs-BA, and BS-vs-BA, respectively. The Gene Ontology (GO) enrichment analysis of DEGs revealed that the regulation of cyclin-dependent protein serine/threonine kinase activity, oxidative phosphorylation, and respiratory chain were significantly enriched. The significant enrichment analysis of the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway identified pathways associated with the regulation of death, such as the mammalian target of rapamycin (mTOR) signaling pathway, AMPK signaling pathway, oxidative phosphorylation, and cell cycle. Conclusion The post-reproductive death of S. esculenta was found to be a complex energy steady-state regulation network system. The mTOR acted as an energy receptor and had a key role in regulating energy homeostasis.
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Ci Dunwei,Tang Zhaohui,Ding Hong,Cui Li,Zhang Guanchu,Li Shangxia,Dai Liangxiang,Qin Feifei,Zhang Zhimeng,Yang Jishun,Xu Yang 한국미생물학회 2021 The journal of microbiology Vol.59 No.1
Peanut (Arachis hypogaea. L) is an important oil seed crop. Both arbuscular mycorrhizal fungi (AMF) symbiosis and calcium (Ca2+) application can ameliorate the impact of saline soil on peanut production, and the rhizosphere bacterial communities are also closely correlated with peanut salt tolerance; however, whether AMF and Ca2+ can withstand high-salinity through or partially through modulating rhizosphere bacterial communities is unclear. Here, we used the rhizosphere bacterial DNA from saline alkali soil treated with AMF and Ca2+ alone or together to perform high-throughput sequencing of 16S rRNA genes. Taxonomic analysis revealed that AMF and Ca2+ treatment increased the abundance of Proteobacteria and Firmicutes at the phylum level. The nitrogenfixing bacterium Sphingomonas was the dominant genus in these soils at the genus level, and the soil invertase and urease activities were also increased after AMF and Ca2+ treatment, implying that AMF and Ca2+ effectively improved the living environment of plants under salt stress. Moreover, AMF combined with Ca2+ was better than AMF or Ca2+ alone at altering the bacterial structure and improving peanut growth in saline alkali soil. Together, AMF and Ca2+ applications are conducive to peanut salt adaption by regulating the bacterial community in saline alkali soil.
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Shanlin Yu,Na Chen,Maowen Su,Xiaoyuan Chi,Zhimeng Zhang,Lijuan Pan,Mingna Chen,Tong Wang,Mian Wang,Zhen Yang 한국유전학회 2016 Genes & Genomics Vol.38 No.6
The cultivated peanut is important oil crop and salt stress seriously influences its development and yield. Tolerant varieties produced using transgenic techniques can effectively increase peanut plantation area and enhance its yields. However, little is known about how gene expression is regulated by salt stress in peanut. In this study, we screened genes regulated by salt stress in peanut roots using microarray technique. In total, 4828 up-regulated and 3752 down-regulated probe sets were successfully identified in peanut roots subjected to 3 and 48 h of salt stress. Data analysis revealed that different response groups existed between the up and down-regulated probe sets. The main up-regulated biological processes involved in salt stress responses included transcription regulation, stress response, and metabolism and biosynthetic processes. The main down-regulated biological processes included transport processes, photosynthesis and development. The Kyoto encyclopedia of genes and genomes pathway analysis indicated that metabolic pathway, biosynthesis of unsaturated fatty acids and plant–pathogen interaction, were mainly up-regulated in peanut under salt stress. However, photosynthesis and phenylalanine metabolism were mainly down-regulated during salt stress. The function of some probe sets in salt stress regulation was not clarified (e.g., protein functioning in cell cycle regulation and xylem development). Many of the genes we identified lacked functional annotations and their roles in response to salt stress are yet to be elucidated. These results identified some candidate genes as potential markers and showed an overview of the transcription map, which may yield some useful insights into salt-mediated signal transduction pathways in peanut.
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