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Jianmei Zhang,Jinyi Zhou,Qiaoyun Yuan,Changyi Zhan,Zhi Shang,Qian Gu,Ji Zhang,Guangbo Fu,Weicheng Hu 고려인삼학회 2021 Journal of Ginseng Research Vol.45 No.2
Backgroud: Ginsenoside compound K (GK) is a major metabolite of protopanaxadiol-type ginsenosides and has remarkable anticancer activities in vitro and in vivo. This work used an ionic cross-linking method to entrap GK within O-carboxymethyl chitosan (OCMC) nanoparticles (Nps) to form GK-loaded OCMC Nps (GK―OCMC Nps), which enhance the aqueous solubility and stability of GK. Methods: The GK―OCMC Nps were characterized using several physicochemical techniques, including x-ray diffraction, transmission electron microscopy, zeta potential analysis, and particle size analysis via dynamic light scattering. GK was released from GK―OCMC Nps and was conducted using the dialysis bag diffusion method. The effects of GK and GK―OCMC Nps on PC3 cell viability were measured by using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide assay. Fluorescent technology based on Cy5.5-labeled probes was used to explore the cellular uptake of GK―OCMC Nps. Results: The GK―OCMC NPs had a suitable particle size and zeta potential; they were spherical with good dispersion. In vitro drug release from GK―OCMC NPs was pH dependent. Moreover, the in vitro cytotoxicity study and cellular uptake assays indicated that the GK―OCMC Nps significantly enhanced the cytotoxicity and cellular uptake of GK toward the PC3 cells. GK―OCMC Nps also significantly promoted the activities of both caspase-3 and caspase-9. Conclusion: GK―OCMC Nps are potential nanocarriers for delivering hydrophobic drugs, thereby enhancing water solubility and permeability and improving the antiproliferative effects of GK.
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Cheng Zhiping,Wang Kaifang,Li Zhongwen,Wu Haotian,Zhi Changyi,Zhang Jinbin 대한전기학회 2024 Journal of Electrical Engineering & Technology Vol.19 No.1
In this paper, distributed secondary control of AC microgrid (MG) is studied and the infuence of communication delay on its control performance is analyzed and verifed. Firstly, a secondary control strategy for the MG is designed to achieve frequency recovery and proportional active power dispatch. Secondly, the stability of the MG system is analyzed in the frequency domain and a rigorous formula is derived to calculate the delay margin for the stability of the distributed secondary control with communication delays. The theoretical calculation results of the stability margin are approximately consistent with the simulation results, with an error of only 1.27%. The efects of the algorithm gains, communication topology, and connection weights on the delay margin are discussed based on the derived formula. The results demonstrate that the algorithm gain is inversely proportional to the delay margin. Changes in the communication topology and connection weights lead to changes in the maximum eigenvalue of the adjacency matrix L, which in turn afects the delay margin. Moreover, the infuence of time-varying delays is considered. The system can tolerate greater time-varying delays than fxed delays. Simulation and experimental results validate the efectiveness and feasibility of the proposed method.
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