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- 저자 : Zhenming LI (검색결과 5 건)
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Fang Gong,Tong Zhang,Jun Sheng,Jing Li,Xianghong Wang,Zhenming Chi 한국생물공학회 2008 Biotechnology and Bioprocess Engineering Vol.13 No.5
The extracellular inulinase of the marine yeast Pichia guilliermondii strain 1 was purified to homogeneity resulting in a 7.2-fold increase in specific inulinase activity. The molecular mass of the purified enzyme was estimated to be 50.0 kDa. The op-timal pH and temperature for the purified enzyme were 6.0 and 60C, respectively. The enzyme was activated by Mn²+, Ca²+, K+, Li+, Na+, Fe³+, Fe²+, Cu²+, and Co²+, but Mg²+, Hg²+, and Ag+ inhibited activity. The enzyme was strongly inhibited by phenylmethanesulphonyl fluoride (PMSF), iodoacetic acid, EDTA, and 1, 10-phenanthroline. The Km and Vmax values of the purified inulinase for inulin were 21.1 mg/mL and 0.08 mg/min, respectively. A large number of monosaccharides were de-tected after the hydrolysis of inulin. The deduced protein sequence from the cloned P. guilliermondii strain 1 inulinase gene contained the consensus motifs R-D-P-K-V-F-W-H and W-M-N-D-P-N-G, which are conserved among the inulinases from other microorganisms.
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UAVs Cooperative Attack Based on Archimedes Spiral
Zhenming LI,Chaoyong Li,Shuo Zhuo,Sai Chen 제어로봇시스템학회 2017 제어로봇시스템학회 국제학술대회 논문집 Vol.2017 No.10
This paper investigates a control of track based on Archimedes spiral. The objective is to control UAVs (Unmanned Aerial Vehicle) circled to the target under the preset track and in a certain time interval. For the circumstance of the known location of start point and target point of UAV, we put forward a control method of angular velocity, with fixed velocity and trajectory of UAV. And in this control method, based on the nature of the Archimedes spiral, the time of UAV from the start point to the target point can be accurately calculated.
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Yingying Ye,Zeqin Fu,Yunfang Tian,Jiji Li,Baoying Guo,Zhenming Lv,Changwen Wu 한국유전학회 2018 Genes & Genomics Vol.40 No.11
Pelagic larval dispersal habits influence the population genetic structure of marine mollusk organisms via gene flow. The genetic information of the clam Gomphina aequilatera (short larval stage, 10 days) which is ecologically and economically important in the China coast is unknown. To determine the influence of planktonic larval duration on the genetic structure of G. aequilatera. Mitochondrial markers, cytochrome oxidase subunit i (COI) and 12S ribosomal RNA (12S rRNA), were used to investigate the population structure of wild G. aequilatera specimens from four China Sea coastal locations (Zhoushan, Nanji Island, Zhangpu and Beihai). Partial COI (685 bp) and 12S rRNA (350 bp) sequences were determined. High level and significant FST values were obtained among the different localities, based on either COI (FST = 0.100–0.444, P < 0.05) or 12S rRNA (FST = 0.193–0.742, P < 0.05), indicating a high degree of genetic differentiation among the populations. The pairwise Nm between Beihai and Zhoushan for COI was 0.626 and the other four pairwise Nm values were > 1, indicating extensive gene flow among them. The 12S rRNA showed the same pattern. AMOVA test results for COI and 12S rRNA indicated major genetic variation within the populations: 77.96% within and 22.04% among the populations for COI, 55.73% within and 44.27% among the populations for 12S rRNA. A median-joining network suggested obvious genetic differentiation between the Zhoushan and Beihai populations. This study revealed the extant population genetic structure of G. aequilatera and showed a strong population structure in a species with a short planktonic larval stage.
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Research on Automatic Mapping Method of Geologic Column
Shao YanLin,Shao YaXiong,Xu XiaoHong,He ZhenMing,Li GongQuan 보안공학연구지원센터 2015 International Journal of Hybrid Information Techno Vol.8 No.7
The realization of the digital reservoir can greatly improve the information level of the petroleum corporation in hydrocarbon exploration and development, reduce risk and improve efficiency. Wells are the core object of digital reservoir management. Geologic column of drilling is the main carrier of multi-source information. Automatic mapping method and multi-source information fusion of geologic column have become a research hotspot of digital reservoir construction. This paper describes current situation of the domestic reservoir management and practical problem of geological maps digitizing. The thought and process of geologic column automatic mapping, which are based on spatial information technology, are submitted. The partition of information bar is settled on. The three-tier system architecture of automatic mapping system is established. With the support of XML technology, XSD is used to make the parameter template of geologic column automatic mapping and realize corresponding configuration wizard. UML is used to design the automatic mapping system. The lithological plate and geological symbols is coded to realize automatic mapping of corresponding drawing elements. The drawing methods of logging and petrophysical data are improved and the visualization of curve and numerical stick figure is achieved. The integration method of casting photos, core images and geologic column is submitted. For the secondary development technology of ArcGIS Engine, object-oriented program is adopted to realize automatic mapping of geologic ArcGIS Engine column. This paper provides a new way for the geologic column mapping during the process of digital reservoir construction.
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Anming Wang,Fangkai Zhang,Feifei Chen,Meizhang Wang,Haifeng Li,Zhaowu Zeng,Tian Xie,Zhenming Chen 한국화학공학회 2011 Korean Journal of Chemical Engineering Vol.28 No.4
To obtain robust and thermo-stable enzyme aggregates, p-benzoquinone was used as cross-linker and bovine serum albumin (BSA) as crowding macromolecules to prepare cross-linked enzyme aggregates (CLEAs) of lipase. Effects of cross-linking time and cross-linker content on the activity, thermal stability and characteristics of enzyme aggregates were examined carefully. It was observed that when the content of p_benzoquinone was 5 mM and amount of BSA was 125% of that of lipase (w/w), the specific activity of cross-linked co-aggregates of lipase and BSA was 79.8 U mg^−1, 2.44-fold of that of cross-linked enzyme aggregates of lipase without BSA. Moreover, after heat treatment for 96 h at 50℃ , the CLEAs prepared with this facile routine kept 75.18% of their initial activity, 5.01-fold more than that of the just CLEAs using glutaraldehyde. Furthermore, BSA macromolecules in lipase CLEAs enhanced the catalytic efficiency of free and just lipase CLEAs without BSA by 1.45 and 2.83 times, respectively. The proposed crosslinking technique would rank among the potential strategies for efficiently preparing robust and thermo-stable enzyme aggregates.
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