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Yuan Liping,Yang Xiao,He Ying,Zhao Yanwen,Chen Yi,Yang Yongshou,Xiao Peiyun 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.2
Periplaneta americana L. (Blattidae) extract (PA-B) possesses anti-oxidant, anti-inflammatory, and hep atoprotective properties; however, its mechanism of action in liver fibrosis remains unclear. Herein, we inves tigated the hepatoprotective effect and mechanism of action of PA-B. HSC-T6 cells treated with PA-B were stimulated with TGF-β1, and immunofluorescence, western blotting, Hoechst 33,342 staining, confocal laser scanning microscopy, and flow cytometry were performed. Furthermore, the effects of PA-B were verified in a rat model of hepatic fibrosis established using 40 % carbon tetrachloride (CCl 4 ). PA-B induced apoptosis of HSC-T6 cells by inhibiting proliferation (maximum inhibition ratio 99.08 %), reducing mitochondrial membrane po tential, and ameliorating apoptosis-related proteins (P < 0.01). PA-B (120 mg/kg) significantly alleviated CCl 4 -induced hepatic histopathological fibrosis and reduced the levels of aspartate aminotransferase (from 27.68 to 20.58 U/L) and alanine aminotransferase (from 16.88 to 5.93 U/L; P < 0.05 or P < 0.01). Immunohistochemistry and western blotting showed that α-SMA levels were reduced (from 0.49 to 0.23) and that the Bcl/Bax signaling pathway was significantly inhibited by PA-B (120 mg/kg; P < 0.05 or P < 0.01). PA-B exhibits anti-fibrotic effects by mediating the Bcl-2/Bax pathway and promoting HSC-T6 cell apoptosis, providing a basis for its development as a drug to treat liver fibrosis.
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Yating Chen,Kaichuang Shi,Huixin Liu,Yanwen Yin,Jing Zhao,Feng Long,Wenjun Lu,Hongbin Si 대한수의학회 2021 Journal of Veterinary Science Vol.22 No.6
Background: African swine fever virus (ASFV), classical swine fever virus (CSFV), and porcine reproductive and respiratory syndrome virus (PRRSV) are still prevalent in many regions of China. Co-infections make it difficult to distinguish their clinical symptoms and pathological changes. Therefore, a rapid and specific method is needed for the differential detection of these pathogens. Objectives: The aim of this study was to develop a multiplex real-time quantitative reverse transcription polymerase chain reaction (multiplex qRT-PCR) for the simultaneous differential detection of ASFV, CSFV, and PRRSV. Methods: Three pairs of primers and TaqMan probes targeting the ASFV p72 gene, CSFV 5′ untranslated region, and PRRSV ORF7 gene were designed. After optimizing the reaction conditions, including the annealing temperature, primer concentration, and probe concentration, multiplex qRT-PCR for simultaneous and differential detection of ASFV, CSFV, and PRRSV was developed. Subsequently, 1,143 clinical samples were detected to verify the practicality of the assay. Results: The multiplex qRT-PCR assay could specifically and simultaneously detect the ASFV, CSFV, and PRRSV with a detection limit of 1.78 × 100 copies for the ASFV, CSFV, and PRRSV, but could not amplify the other major porcine viruses, such as pseudorabies virus, porcine circovirus type 1 (PCV1), PCV2, PCV3, foot-and-mouth disease virus, porcine parvovirus, atypical porcine pestivirus, and Senecavirus A. The assay had good repeatability with coefficients of variation of intra- and inter-assay of less than 1.2%. Finally, the assay was used to detect 1,143 clinical samples to evaluate its practicality in the field. The positive rates of ASFV, CSFV, and PRRSV were 25.63%, 9.36%, and 17.50%, respectively. The co-infection rates of ASFV+CSFV, ASFV+PRRSV, CSFV+PRRSV, and ASFV+CSFV+PRRSV were 2.45%, 2.36%, 1.57%, and 0.17%, respectively. Conclusions: The multiplex qRT-PCR developed in this study could provide a rapid, sensitive, specific diagnostic tool for the simultaneous and differential detection of ASFV, CSFV, and PRRSV.
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Implicit Detection of Hidden Processes with a Local-Booted Virtual Machine
Yan Wen,Huaimin Wang,Jinjing Zhao 보안공학연구지원센터 2008 International Journal of Security and Its Applicat Vol.2 No.4
Currently stealth malware is becoming a major threat to the PC computers. Process hiding is the technique commonly used by stealth malware to evade detection by anti-malware scanners. On the defensive side, previous host-based approaches will be defeated once the privileged stealth malware controls a lower reach of the system. The virtual machine (VM) based solutions gain tamper resistance at the cost of losing the OS-level process view. Moreover, existing VM-based approaches cannot introspect the preinstalled OS which is just the protecting concern for PC users. In this paper, we present a new VM-based approach called Libra which accurately reproduces the software environment of the underlying preinstalled OS within the Libra VM and provides an OS-level semantic view of the processes. With our new local-booting technology, Libra VM just boots from the underlying host OS but not a newly installed OS image. Thus, Libra provides a way to detect the existing process-hiding stealth malware in the host OS. In addition, instead of depending on the guest information which is subvertable to the privileged guest malware, Libra adopts a unique technique to implicitly construct the Trusted View of Process List (TVPL) from within the virtualized hardware layer. Our evaluation results with real-world hiding-process rootkits, which are widely used by stealth malware, demonstrate its practicality and effectiveness.
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