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        Genome-wide analysis of the GRAS gene family exhibited expansion model and functional differentiation in sea buckthorn (Hippophae rhamnoides L.)

        Yu Liyang,Zhang Guoyun,Lyu Zhongrui,He Caiyun,Zhang Jianguo 한국식물생명공학회 2021 Plant biotechnology reports Vol.15 No.4

        Key message Genome-wide identification of the sea buckthorn GRAS genes and their transcription profiles in dif- ferent organizations. Abstract GRAS proteins comprise a large family of transcription factors that experienced extensive replication, and play important roles in many aspects of growth regulatory and environmental signals. Sea buckthorn is a unique and valuable plant which has tremendous value for medical researching, ecological protection, and providing daily necessities. We iden- tified, characterized, and distinguished duplication models of GRAS transcription factors in sea buckthorn. A systematic whole-genome analysis was performed to identify and characterize the GRAS gene family of sea buckthorn, especially the comparative genome method was used to demonstrate its different duplication models. We identified 62 sea buckthorn GRAS (HrGRAS) genes and renamed based on their respective chromosome distribution. Fifty-nine HrGRASs were classified into nine subgroups and three HrGRASs did not belong to any of the subfamilies according to their phylogenetic features. HrGRAS genes tend to have a representative GRAS domain, few introns, and unevenly distributed on chromosomes. Seg- mental duplication was the main driver of the GRAS gene family expansion, followed by whole-genome duplication (WGD) and tandem duplication, according to the results of comparative genome analysis. GRAS genes involved in duplication expe- rienced strong purifying selection pressure according to the Ka/Ks ratios. The expression profile derived from transcriptome data shows the expression patterns of HrGRAS gene in different tissues and the characterization of differentially expressed genes. This systematic analysis provided a foundation to understand the expansion and potential functions of GRAS genes with an aim of sea buckthorn crop improvement.

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        Dual-anchor anti-corrosion coating of copper foil for high-speed interconnects

        Huijuan Shi,Guoyun Zhou,Qin Zhang,Pengju Wang,Yan Hong,Wei He,Shouxu Wang,Chong Wang,Zhiwei Han 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.126 No.-

        In this experiment, two similar molecules with bifunctional groups have been characterized to prove theadvantages of dual-anchor molecular coating in the interfacial modification of the copper foil. The physicochemicalproperties of the modified copper foils have been studied. The obtained results indicate thatthe anti-corrosion performance and peeling strength were significantly improved for the copper foil treatedwith (2-(Methylthio)pyrimidin-4-yl)methanamine (MET), whose bonding information was analyzedvia the density functional theory calculation. It was found that the S-Cu and N-Cu bonds were bothformed on the Cu (1 1 1) surface for MET coating, and the adsorption of the double anchor pointsimproves the stability of the interface. The peeling test exhibits the function of MET coating as the adhesionpromotor between low-surface profile copper and resin for high-speed signal transmission.

      • KCI등재

        Insights into the evolutionary origin and expansion of the BBX gene family

        Yu Liyang,Lyu Zhongrui,Liu Hong,Zhang Guoyun,He Caiyun,Zhang Jianguo 한국식물생명공학회 2022 Plant biotechnology reports Vol.16 No.2

        The B-box proteins are a class of zinc finger transcription factors and play important roles in regulating plant growth, devel- opment, and stress response. However, their origin and expansion model in plants have been very vague. In this study, 208 B-box genes were identified in 18 plant species, and phylogenetic analyses divided them into five structure groups. Subse- quently, the sequence analysis including theoretical isoelectric point, instability index, and aliphatic index showed the wide variation of B-box gene in different species. Our multispecies genome-wide analysis reveals that the structure groups III and IV in the plant BBX gene family have the earliest origin (Rhodophyta) and are extensively expanded in land plants, while the other three structure groups (I, II, and V) seem to originate at least in the last common ancestor of land plants. Further- more, whole genome duplication (WGD) was the main driver (28 gene pairs, 65.12%) of the B-box gene family expansion, followed by segmental duplication, which tend to have more introns and are subject to more intense purification selections. We also analyzed the sequence differences between B-box domains to propose a new evolutionary model of B-box domain. These analyses provide new insights for understanding the origin and evolution of the B-box gene family.

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