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Incomplete autophagy promotes the replication of Mycoplasma hyopneumoniae
Wang Zhaodi,Wen Yukang,Zhou Bingqian,Tian Yaqin,Ning Yaru,Ding Honglei 한국미생물학회 2021 The journal of microbiology Vol.59 No.8
Autophagy is an important cellular homeostatic mechanism for recycling of degradative proteins and damaged organelles. Autophagy has been shown to play an important role in cellular responses to bacteria and bacterial replication. However, the role of autophagy in Mycoplasma hyopneumoniae infection and the pathogenic mechanism is not well characterized. In this study, we showed that M. hyopneumoniae infection significantly increases the number of autophagic vacuoles in host cells. Further, we found significantly enhanced expressions of autophagy marker proteins (LC3-II, ATG5, and Beclin 1) in M. hyopneumoniae-infected cells. Moreover, immunofluorescence analysis showed colocalization of P97 protein with LC3 during M. hyopneumoniae infection. Interestingly, autophagic flux marker, p62, accumulated with the induction of infection. Conversely, the levels of p62 and LC3-II were decreased after treatment with 3-MA, inhibiting the formation of autophagosomes, during infection. In addition, accumulation of autophagosomes promoted the expression of P97 protein and the survival of M. hyopneumoniae in PK- 15 cells, as the replication of M. hyopneumoniae was downregulated by adding 3-MA. Collectively, these findings provide strong evidence that M. hyopneumoniae induces incomplete autophagy, which in turn enhances its reproduction in host cells. These findings provide novel insights into the interaction of M. hyopneumoniae and host.
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Dongping Chen,Yashan Li,Yukang Wang,Jinlian Xu 한국유전학회 2021 Genes & Genomics Vol.43 No.2
Background Non-small cell lung cancer (NSCLC) is a major contributor of cancer-related mortality. Long non-coding RNAs (lncRNAs) are indicated to participate in the pathogenesis of NSCLC. Objective In this research, the efects of lncRNA HOXA transcript antisense RNA, myeloid-specifc 1 (HOTAIRM1) on NSCLC progression and underlying mechanism were revealed. Methods The expression levels of HOTAIRM1 and microRNA-498 (miR-498) were detected by quantitative real time polymerase chain reaction (qRT-PCR) in NSCLC tissues, cells or exosomes. The protein expression of CD63, CD81, hexokinase 2 (HK2) and ATP binding cassette subfamily E member 1 (ABCE1) was determined by western blot. Cell viability, apoptosis, migration and invasion were investigated by cell counting kit-8 (CCK-8), fow cytometry, transwell migration and invasion assays, respectively. Cell glycolysis metabolism was revealed by glucose uptake and lactate production assays and western blot analysis. The binding relationship between miR-498 and HOTAIRM1 or ABCE1 was predicted by DIANA-LncBase v2 and starBase online database, and identifed by dual-luciferase reporter assay. The efects of HOTAIRM1 on NSCLC growth in vivo were revealed by in vivo tumor formation assay. Results HOTAIRM1 expression was dramatically upregulated, whereas miR-498 expression was signifcantly downregulated in NSCLC tissues cells or exosomes as compared to control groups. Mechanistically, HOTAIRM1 knockdown repressed cell viability, migration, invasion and glycolysis metabolism, whereas induced cell apoptosis in NSCLC; however, miR498 inhibitor hindered these efects. Functionally, HOTAIRM1 functioned as a sponge of miR-498 and miR-498 targeted ABCE1. In addition, HOTAIRM1 silencing inhibited NSCLC growth in vivo by downregulating ABCE1 and upregulating miR-498 expression. Conclusions HOTAIRM1 knockdown repressed cell glycolysis metabolism and tumor development by reducing ABCE1 expression through sponging miR-498 in NSCLC, which provided a theoretical basis for further studying NSCLC progression.
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Hyeon Jo,Junsik Wang,Jun Yong Lee,Yujung Shin,Yukang Jeong,Jae kwang Lee 한국정보기술학회 2024 한국정보기술학회논문지 Vol.22 No.8
본 연구는 전통적인 구독 서비스 모형에서 확장하여 콘텐츠, 제품 배송, 대여를 통합하는 시스템에 이르기까지 진화한 구독 서비스 환경을 조명한다. 이 연구는 가치 기반 수용 모델을 확장하여 지각된 혜택(유용성, 재미, 콘텐츠 다양성)과 희생(서비스 복잡성, 실망, 지각된 비용)이 전체 구독 서비스 사용 의도에 미치는 영향을 평가한다. 또한, 매개변수로의 지각된 가치의 역할을 실증하고, 개인의 혁신성이 지각된 가치와 서비스 사용 의도의 관계에 미치는 조절 효과를 조사한다. 84명의 응답에 대해 회귀분석을 수행한 결과, 개인의 혁신성이 유의미하고 긍정적인 매개 역할을 하며, 지각된 가치가 사용 의도에 직접적이고 긍정적인 영향을 미친다는 사실이 밝혀졌다. 본 연구 결과는 구독 서비스 채택을 촉진하는 데 있어 지각된 가치와 혁신성의 중요성을 강조하며, 정보 분야에서 소비자 참여를 향상시키기 위한 시사점을 제공한다. During the COVID-19 pandemic, the subscription economy has seen substantial growth, driven by consumer shifts towards safer and more personalized consumption methods. This study delves into the evolving landscape of subscription services, from traditional setups to integrated systems encompassing content, product deliveries, and rentals. Using the value-based acceptance model, the research evaluates how perceived benefits(such as usefulness, playfulness, and content variety) and sacrifices(including service complexity, disappointment, and perceived cost) affect intentions to use total subscription services, with perceived value serving as a mediator. Additionally, the study examines the influence of personal innovativeness on the relationship between perceived value and the intention to use these services. Employing regression analysis on data from 84 users, the results reveal a significant, positive moderating role of personal innovativeness and a direct, positive impact of perceived value on usage intentions. These findings highlight the importance of perceived value and innovativeness in driving adoption of subscription services, offering insights for enhancing consumer engagement in this dynamic sector.
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Haozhan Lia,Hongkang Wei,Huijuan Qiu,Yukang Wanga,Chang-an Wang,Zhipeng Xie 한양대학교 청정에너지연구소 2023 Journal of Ceramic Processing Research Vol.24 No.1
Sub-micron boron carbide powders were synthesized through a gas-solid reaction process using ZIF-8-derived nanoporouscarbon and boron oxide as raw materials. The effects of calcination temperature and atmosphere, amount of boron oxide, andgas-solid reaction apparatus on phase composition and morphology of the synthesized boron carbide powders were studied. The results show that, even with sufficient boron oxide addition, it is not feasible to obtain boron carbide powder of high purityin argon environment. Although vacuum environment can significantly improve the purity of the synthesized boron carbidepowder, there are a large number of large particles of step morphology in the synthesized boron carbide powder. The improvedgas-solid reaction apparatus can effectively impede the formation of boron carbide particles of step morphology. Nearlyspherical submicron boron carbide particles with a median particle size of 480 nm and good particle size consistency couldbe synthesized at 1600 °C with 0.3 g nanoporous carbon and 10 g B2O3 under vacuum by using the improved apparatus
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