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Mercury Ions Mediated Phosphorus Containing Carbon Dots as Fluorescent Probe for Biothiols Screening
Han Du,Hu Xu,Yun Zhao,Dan Li,Yuhong Wang 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2018 NANO Vol.13 No.10
In this study, we report the mercury ions (Hg2+) mediated phosphorus-containing carbon dots (PCDs) as a selective "off–on" fluorescence probe for glutathione (GSH), cysteine (Cys) and homocysteine (Hcys). PCDs obtained by hydrothermal reaction are sensitive to Hg2+ ions and its fluorescence can be significantly quenched owing to the electron transfer from the lowest unoccupied molecular orbital (LUMO) of PCDs to Hg2+. Interestingly, the weak fluorescence of Hg2+-mediated PCDs could be gradually recovered with the addition of GSH, Cys and Hcys. This can be attributed to the formation of Hg2+–S complex due to the super affinity of Hg2+–sulfydryl bond. The formation of Hg2+–S complex extremely reduces the oxidation ability of Hg2+ that inhibits the electron transfer from LUMO of PCDs to Hg2+ and re-opens the native electron transition from LUMO to the highest occupied molecular orbital (HOMO) of PCDs. Thus, the green fluorescence of PCDs is switched on. Furthermore, the present Hg2+-mediated PCDs assay exhibits a high selectivity for GSH, Cys and Hcy and has been successfully used to detect the total biothiols content in human urine samples.
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Prospects and challenges of epigenomics in crop improvement
Huang Yuhong,Liu Yang,Liu Chang,Birchler James A.,Han Fangpu 한국유전학회 2022 Genes & Genomics Vol.44 No.3
Background: The advent of high-throughput epigenome mapping techniques has ushered in a new era of multiomics with powerful tools now available to map and record genomic output at different levels. Integrating the different components of the epigenome from these multiomics measures allows investigations of cis-regulatory elements on a genome-scale. Mapping of chromatin state, chromatin accessibility dynamics, and higher-order chromatin structure enables a new level of understanding of cell fate determination, identity and function in normal growth and development, disease resistance, and yield. Objective: In this paper, the recent advances in epigenomics research of rice, maize, and wheat are reviewed, and the development trends of epigenomics of major crops in the coming years are projected. Methods: We highlight the role of epigenomics in regulating growth and development and identifying potential distal cis-regulatory elements in three major crops, and discuss the prospects and challenges for new epigenetics-mediated breeding technologies in crop improvement. Conclusion: In this review, we summarize and analyze recent epigenomic advances in three major crops epigenomics and discuss possibilities and challenges for future research in the field.
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Yi Zhao,Tianhao Wang,Yongbin Wang,Runlong Hao,Han Wang,Yuhong Han 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.77 No.-
Carbon dioxide (CO2) was converted into formate (HCO2) in a catalytic reduction system mainlycomposed of potassium borohydride (KBH4) and nanoscale bimetallic nickel–copper alloy (NBN–C), inwhich, the average CO2 conversion efficiency of 41.92% was obtained under the optimal experimentalconditions, with a HCO2selectivity of 53.42%. Various characterization methods were employed toinvestigate the physicochemical properties of NBN–C and the results indicated that NBN–C was a coreshellmesoporous catalyst with key active sites containing Ni0 and Cu0, at which the catalytic reduction ofCO2 took place. The reaction mechanism was proposed based on these characterizations and relevantliteratures.
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