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Siyao Wang,Yuejuan Zhang,Dong Gao,Jing Zi,Wenpeng Wang,Nianzhe Zhang,Yi Wan,Lili Wang 한국생물공학회 2019 Biotechnology and Bioprocess Engineering Vol.24 No.4
Streptavidin has applied to many areas including detection, purification, labeling, crosslinking and immobilization resulting in a high demand on its production. In this study, we report a method for preparation of recombinant core streptavidin (cSAV) protein using highperformance hydrophobic interaction chromatography (HPHIC). Firstly the cSAV was successfully cloned and expressed in Escherichia coli as inclusion bodies. A bifunctional stationary phase mainly working as HIC mode accompanied by weak anion exchange chromatography (WAX) was prepared using β-phenylethylamine (PEA) as a ligand. The denatured cSAV was then refolded and simultaneously purified by PEA hydrophobic interaction chromatography (PEA-HIC). The mass recovery and purity of cSAV by single-step were 30.2% and 98%, respectively. The bioactivity was determined to be 13.2 U/mg by biotin binding capacity assay. This method provides a new possibility for fast separation with simultaneous renaturation of cSAV.
Fluorination of dichlorodifluoromethane to synthesize tetrafluoromethane over Cr_2O_3–AlF_3 catalyst
Wenzhi Jia,Mengfei Luo,Lingyun Jin,Yuejuan Wang,Jiqing Lu 한국공업화학회 2011 Journal of Industrial and Engineering Chemistry Vol.17 No.3
A series of Cr_2O_3–AlF_3 catalysts with different Cr_2O_3 contents were prepared by a deposition–precipitation method for the fluorination of dichlorodifluoromethane (CCl_2F_2) to synthesize tetrafluoromethane (CF4). The highest activity was obtained on the Cr_2O_3–AlF_3 catalyst containing 61.2 wt.% of Cr_2O_3, with the CF_4 yield of 94% at 400℃. X-ray diffraction (XRD) and Raman spectroscopy results indicated the presence of bulk Cr_2O_3 and a-AlF_3 phases in the catalysts. Ammonia temperature programmed desorption (NH3-TPD) and Fourier transformation infrared spectroscopy (FTIR) of pyridine chemisorption revealed strong Lewis acidity of the catalysts. The Cr species and Lewis acid in the Cr_2O_3–AlF_3 catalysts were the active sites of fluorination and dismutation reaction of CCl_2F_2, respectively. This indicated bi-function catalytic performance of Cr_2O_3–AlF_3 catalysts for fluorination and dismutation reaction.