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      • KCI등재

        Over-expression of GmMYB39 leads to an inhibition of the isoflavonoid biosynthesis in soybean (Glycine max. L)

        Jinxin Yi,Xiaoqing Liu,Lingling Yuan,Ling Xu,Zhaolong Xu,Yihong Huang,Xiaolan He,Hongxiang Ma,Dayong Zhang 한국식물생명공학회 2013 Plant biotechnology reports Vol.7 No.4

        In this paper, we isolated and characterized a gene encoding the soybean MYB transcription factor, GmMYB39 (Accession No: XM_003538605). Analysis of the deduced amino acid sequence revealed that GmMYB39 contained N-terminal R2R3 repeats that corresponded to the DNA-binding domain of plant MYB-type proteins, which were highly conserved among the R2R3-MYB proteins. The detailed expression pattern of GmMYB39 in various tissues of soybean was investigated by quantitative RT-PCR. The transcript level was found to be higher in flowers than in other examined organs. In contrast, the GmMYB39 expression level was relatively weak in the pod. The GmMYB39–GFP fusion protein was found to localize in the nucleus of Arabidopsis mesophyll protoplasts. Over-expression of GmMYB39 in hairy roots resulted in a significant reduction of the transcript levels of PAL, C4H, CHS, 4CL, and CHR. Whereas, the IFS transcript level was slightly but not significantly increased, and no significant change in CHIexpression was observed between over-expression and control roots. The repressing effect on expression of CHS was further supported by the results from co-transfection assays of CHS promoter (reporter) and GmMYB39 (effector) in soybean. Compared to the control (only with the reporter construct), a significant decrease in the GUS activity was observed in soybean hairy roots with both effector and reporter constructs. Furthermore, an ultimate decrease in isoflavonoids contents coincided with the decrease in the transcript levels of PAL, C4H, CHS, 4CL, and CHR. Overall, the results suggest that GmMYB39 plays an inhibiting role in regulating the isoflavonoid biosynthesis in soybean.

      • KCI등재

        Over-expression of GmMYB39 leads to an inhibition of the isoflavonoid biosynthesis in soybean (Glycine max. L)

        Liu, Xiaoqing,Yuan, Lingling,Xu, Ling,Xu, Zhaolong,Huang, Yihong,He, Xiaolan,Ma, Hongxiang,Yi, Jinxin,Zhang, Dayong 한국식물생명공학회 2013 Plant biotechnology reports Vol.7 No.4

        In this paper, we isolated and characterized a gene encoding the soybean MYB transcription factor, GmMYB39 (Accession No: XM_003538605). Analysis of the deduced amino acid sequence revealed that GmMYB39 contained N-terminal R2R3 repeats that corresponded to the DNA-binding domain of plant MYB-type proteins, which were highly conserved among the R2R3-MYB proteins. The detailed expression pattern of GmMYB39 in various tissues of soybean was investigated by quantitative RT-PCR. The transcript level was found to be higher in flowers than in other examined organs. In contrast, the GmMYB39 expression level was relatively weak in the pod. The GmMYB39-GFP fusion protein was found to localize in the nucleus of Arabidopsis mesophyll protoplasts. Over-expression of GmMYB39 in hairy roots resulted in a significant reduction of the transcript levels of PAL, C4H, CHS, 4CL, and CHR. Whereas, the IFS transcript level was slightly but not significantly increased, and no significant change in CHI expression was observed between over-expression and control roots. The repressing effect on expression of CHS was further supported by the results from co-transfection assays of CHS promoter (reporter) and GmMYB39 (effector) in soybean. Compared to the control (only with the reporter construct), a significant decrease in the GUS activity was observed in soybean hairy roots with both effector and reporter constructs. Furthermore, an ultimate decrease in isoflavonoids contents coincided with the decrease in the transcript levels of PAL, C4H, CHS, 4CL, and CHR. Overall, the results suggest that GmMYB39 plays an inhibiting role in regulating the isoflavonoid biosynthesis in soybean.

      • Load Interval Prediction of the Power System based on Type-2 Fuzzy Theory

        He Tao,Liang Zhidong,Pang Jihong,Ye Xianquan,Yuan Jinxin 보안공학연구지원센터 2016 International Journal of Grid and Distributed Comp Vol.9 No.2

        According to the power load has strong randomness and difficult to forecast, the introduction of the two types of fuzzy logic in order to improve the prediction accuracy. The interval type two non-single valued two type Mamdani fuzzy model for power load time series forecasting, and reverse the spread of a similarity of a singular value decomposition iterative blending algorithm to simplify the redundant rules in the model of fuzzy sets and redundant fuzzy rules, in order to eliminate the adverse effects. For ordinary type-2 fuzzy sets, uncertainty of the trace and once the membership function is the most important factor, therefore in the calculation formula for construction of two kinds of measure when considering these two factors; analysis of the ordinary type-2 fuzzy inclusion degree properties; discussed two kinds of conversion between the new measure of the relationship, revealing its internal relations; finally through an example to verify the performance of the new measure ordinary type-2, and the fuzzy similarity and Yang Shih clustering method combining cluster analysis used in Gauss plain type-2 fuzzy sets, obtained the reasonable clustering results, verify the rationality of the new measure and effectiveness.

      • KCI등재

        Active control of amplitude and phase of high-power RF systems in EAST ICRF heating experiments

        Zhu Guanghui,Liu Lunan,Mao Yuzhou,Zhang Xinjun,Guo Yaoyao,Ai Lin,Jiang Runhao,Qin Chengming,Zhang Wei,Yang Hua,Yuan Shuai,Wang Lei,Ju Songqing,Wang Yongsheng,Sun Xuan,Yang Zhida,Wang Jinxin,Cheng Yan 한국원자력학회 2023 Nuclear Engineering and Technology Vol.55 No.2

        The EAST ICRF system operating space has been extended in power and phase control with a low-level RF system for the new double-strap antenna. Then the multi-step power and periodic phase scanning experiment were conducted in L-mode plasma, respectively. In the power scanning experiment, the stored energy, radiation power, plasma impedance and the antenna's temperature all have positive responses during the short ramp-ups of PL;ICRF. The core ion temperature increased from 1 keV to 1.5 keV and the core heating area expanded from jZj 5 cmto jZj 10 cm during the injection of ICRF waves. In the phasing scanning experiment, in addition to the same conclusions as the previous relatively phasing scanning experiment, the superposition effect of the fluctuation of stored energy, radiation power and neutron yield caused by phasing change with dual antenna, resulting in the amplitude and phase shift, was also observed. The active control of RF output facilitates the precise control of plasma profiles and greatly benefits future experimental exploration.

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