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      • Hepato-protective effect of fucoidan extracted from acid-processed Sargassum fusiformis in ethanol-treated Chang liver cells and in a zebrafish model

        Yu-Lin Dai,Yun-Fei Jiang,Yu-Hang Nie,Yu-An Lu,Min-Cheol Kang,You-Jin Jeon 제주대학교 해양과학연구소 2020 해양과환경연구소 연구논문집 Vol.44 No.-

        In our previous study, the anticancer effect of the active fucoidan (JHCF4) isolated from acid-processed Sargassum fusiformis was evaluated. In this study, the liver-protective effects of JHCF4 against ethanol-induced Chang liver cell damage and apoptosis-related responses were investigated. Furthermore, the low cytotoxicity and high cell viability of JHCF4 against ethanol-induced cell damage, as well as its protective effect against ethanol-induced cell apoptosis, were observed via nuclear staining with Hoechst 33342 in Chang liver cells. Additionally, the treatment of the 72 h post-fertilization (hpf) zebrafish model with JHCF4 increased the ethanol-stimulated survival rates as well as decreased oxidative stress, lipid peroxidation, and cell death levels. JHCF4 was found to significantly decrease steatosis production in the 128 hpf zebrafish model by Oil Red O staining, as well as attenuate the malondialdehyde and increase the glutathione contents, compared with the untreated group. These results demonstrate that JHCF4 has a potential hepato-protective effect against ethanol-induced damage both in vitro and in vivo.

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        Theoretical analysis of simply supported channel girder bridges

        Hong-Song Hu,Jian-Guo Nie,Yu-Hang Wang 국제구조공학회 2015 Structural Engineering and Mechanics, An Int'l Jou Vol.56 No.2

        Channel girder bridges that consist of a deck slab and two side beams are good choices for railway bridges and urban rail transit bridges when the vertical clearance beneath the bridge is restricted. In this study, the behavior of simply supported channel girder bridges was theoretical studied based on the theory of elasticity. The accuracy of the theoretical solutions was verified by the finite element analysis. The global bending of the channel girder and the local bending of the deck slab are two contributors to the deformations and stresses of the channel girder. Because of the shear lag effect, the maximum deflection due to the global bending could be amplified by 1.0 to 1.2 times, and the effective width of the deck slab for determining the global bending stresses can be as small as 0.7 of the actual width depending on the width-tospan ratio of the channel girder. The maximum deflection and transversal stress due to the local bending are obtained at the girder ends. For the channel girders with open section side beams, the side beam twist has a negligible effect on the deflections and stresses of the channel girder. Simplified equations were also developed for calculating the maximum deformations and stresses.

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