Species Specific Antiviral Activity of Porcine Interferon-α8 (IFNα8)

Kim, Eunhye,Jhun, Hyunjhung,Kim, Joohee,Park, Unjoo,Jo, Seunghyun,Kwak, Areum,Kim, Sinae,Nguyen, Tam T.,Kang, Yongsun,Choi, Insoo,Lee, Joongbok,Kim, Heijun,Kim, Younghyun,Lee, Siyoung,Kim, Soohyun 한국조명·전기설비학회 2017 한국조명·전기설비학회 학술대회논문집 Vol. No.

<P>Interferons (IFNs) have been known as antiviral genes and they are classified by type 1, type 2, and type 3 IFN. The type 1 IFN consists of IFNα, IFNβ, IFNτ, and IFNω whereas the type 2 IFN consists of only IFNγ, which is a key cytokine driving T helper cell type 1 immunity. IFNλ belongs to the type 3 IFN, which is also known as IL-28 and IL-29 possessing antiviral activities. Type 1 IFN is produced by viral infection whereas type 2 IFN is induced by mitogenic or antigenic T-cell stimuli. The IFNτ of bovine was first discovered in an ungulate ruminant recognition hormone. IFNτ belongs to the type 1 IFN with the common feature of type 1 IFN such as antiviral activity. IFNs have been mostly studied for basic research and clinical usages therefore there was no effort to investigate IFNs in industrial animals. Here we cloned porcine IFNα8 from peripheral blood mononuclear cells of Korean domestic pig (<I>Sus scrofa domestica</I>). The newly cloned IFNα8 amino acid sequence from Korean domestic pig shares 98.4% identity with the known porcine IFNα8 in databank. The recombinant porcine IFNα8 showed potent antiviral activity and protected bovine Madin-Darby bovine kidney epithelial (MDBK) cells from the cytopathic effect of vesicular stomatitis virus, but it failed to protect human Wistar Institute Susan Hayflick (WISH) cells and canine Madin-Darby canine kidney epithelial-like (MDCK) cells. The present study demonstrates species specific antiviral activity of porcine IFNα8.</P>

Comparison of viability and antioxidant capacity between canine adipose-derived mesenchymal stem cells and heme oxygenase-1-overexpressed cells after freeze-thawing

KIM, Mijung,KIM, Yongsun,LEE, Seunghoon,KUK, Minyoung,KIM, Ah Young,KIM, Wanhee,KWEON, Oh-Kyeong The Japanese Society of Veterinary Science 2016 The Journal of veterinary medical science Vol.78 No.4

<P>Allogenic adipose-derived mesenchymal stem cells (Ad-MSCs) are an alternative source for cytotherapy owing to their antioxidant and anti-inflammatory effects. Frozen-thawed allogenic Ad-MSCs can be used instantly for this purpose. However, the viability and function of frozen-thawed Ad-MSCs have not been clearly evaluated. The purpose of this study was to compare the viability and function of Ad-MSCs and heme oxygenase-1 (HO-1)-overexpressed Ad-MSCs <I>in vitro</I> after freeze-thawing. The viability, proliferation, antioxidant capacity and mRNA gene expression of growth factors were evaluated. Frozen-thawed cells showed significantly lower viability than fresh cells (77% for Ad-MSCs and 71% for HO-1 Ad-MSCs, <I>P</I><0.01). However, the proliferation rate of frozen-thawed Ad-MSCs increased and did not differ from that of fresh Ad-MSCs after 3 days of culture. In contrast, the proliferation rate of HO-1-overexpressed Ad-MSCs was lower than that of Ad-MSCs. The mRNA expression levels of <I>TGF-β</I>, <I>HGF</I> and <I>VEGF</I> did not differ between fresh and frozen-thawed Ad-MSCs, but <I>COX-2</I> and <I>IL-6</I> had significantly higher mRNA expression in frozen cells than fresh cells (<I>P</I><0.05). Fresh Ad-MSCs exhibited higher <I>HO-1</I> mRNA expression than frozen-thawed Ad-MSCs, and fresh HO-1-overexpressed Ad-MSCs exhibited higher than fresh Ad-MSCs (<I>P</I><0.05). However, there was no significant difference between fresh and frozen HO-1-overexpressed Ad-MSCs. The antioxidant capacity of HO-1-overexpressed Ad-MSCs was significantly higher than that of Ad-MSCs. Cryopreservation of Ad-MSCs negatively affects viability and antioxidant capacity, and HO-1-overexpressed Ad-MSCs might be useful to maximize the effect of Ad-MSCs for cytotherapy.</P>

Effect of Gelatin on Osteogenic Cell Sheet Formation Using Canine Adipose-Derived Mesenchymal Stem Cells

Kim, Ah Young,Kim, Yongsun,Lee, Seung Hoon,Yoon, Yongseok,Kim, Wan-Hee,Kweon, Oh-Kyeong SAGE Publications 2017 Cell transplantation Vol.26 No.1

Mapping potential landscapes of semiconducting carbon nanotubes with scanning gate microscopy

Kim, Yongsun,Oh, Young Mu,Park, Ji-Yong,Kahng, Se-Jong IOP Pub 2007 Nanotechnology Vol.18 No.47

<P>Inhomogeneities in the energy bands of semiconducting carbon nanotubes (CNTs) are investigated with scanning gate microscopy (SGM). Non-uniform responses of a semiconducting CNT to a local electric field are found in SGM images which are obtained at the different depletion states of the CNT. The results indicate that local potential perturbations affect the transport characteristics of semiconducting CNTs significantly, especially near the off-state of the device.</P>

Evaluation of Mesenchymal Stem Cell Sheets Overexpressing BMP-7 in Canine Critical-Sized Bone Defects

Kim, Yongsun,Kang, Byung-Jae,Kim, Wan Hee,Yun, Hui-suk,Kweon, Oh-kyeong MDPI 2018 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.19 No.7

<P>The aim of this study was to investigate the in vitro osteogenic capacity of bone morphogenetic protein 7 (BMP-7) overexpressing adipose-derived (Ad-) mesenchymal stem cells (MSCs) sheets (BMP-7-CS). In addition, BMP-7-CS were transplanted into critical-sized bone defects and osteogenesis was assessed. BMP-7 gene expressing lentivirus particles were transduced into Ad-MSCs. BMP-7, at the mRNA and protein level, was up-regulated in BMP-7-MSCs compared to expression in Ad-MSCs. Osteogenic and vascular-related gene expressions were up-regulated in BMP-7-CS compared to Ad-MSCs and Ad-MSC sheets. In a segmental bone-defect model, newly formed bone and neovascularization were enhanced with BMP-7-CS, or with a combination of BMP-7-CS and demineralized bone matrix (DBM), compared to those in control groups. These results demonstrate that lentiviral-mediated gene transfer of BMP-7 into Ad-MSCs allows for stable BMP-7 production. BMP-7-CS displayed higher osteogenic capacity than Ad-MSCs and Ad-MSC sheets. In addition, BMP-7-CS combined with demineralized bone matrix (DBM) stimulated new bone and blood vessel formation in a canine critical-sized bone defect. The BMP-7-CS not only provides BMP-7 producing MSCs but also produce osteogenic and vascular trophic factors. Thus, BMP-7-CS and DBM have therapeutic potential for the treatment of critical-sized bone defects and could be used to further enhance clinical outcomes during bone-defect treatment.</P>

Power Controlled Concurrent Transmissions in mmWave WPANs

KIM, Yongsun,KIM, Meejoung,LEE, Wooyong,KANG, Chul-Hee The Institute of Electronics, Information and Comm 2010 IEICE TRANSACTIONS ON COMMUNICATIONS - Vol.93 No.10

<P>This letter considers power-controlled transmission from directional antennas in mmWave wireless personal area network (WPAN) systems. The attributes of these systems are studied; these include the number of concurrent transmissions and the power consumption with different system parameters, such as the antenna's beamwidth and radiating efficiency. Numerical results are presented to show that the power controlled transmission enables more concurrent transmissions than the non-power controlled transmission. The results also show that the number of concurrent transmissions increases as the beamwidth and the path loss component become smaller and the antenna's radiating efficiency increases. In addition, the power controlled system generally uses less power than the non-power controlled transmission set up; the overall analysis is verified by simulation.</P>

초정밀 서비스를 위한 절대시간 동기 절차 연구

김용선(Yongsun Kim),장갑석(Kapseok Chang),고영조(Youngjo Ko),이상호(Sangho Lee) 한국통신학회 2020 한국통신학회 학술대회논문집 Vol.2020 No.2

스마트그리드 서비스를 위한 절대시간동기 절차 연구

김용선(Yongsun Kim),장갑석(Kapseok Chang),고영조(Youngjo Ko) 한국통신학회 2020 한국통신학회 학술대회논문집 Vol.2020 No.8

대형 자동차 프레임의 구조해석

주용선(Yongsun Ju),김영권(Youngkweon Kim),김방원(Bangwon Kim),문정오(Jeongoh Moon),이기수(Kisu Lee) 한국자동차공학회 2003 한국자동차공학회 춘 추계 학술대회 논문집 Vol.- No.-

This paper presents the static analysis on the stress of a heavy- duty truck frame which is made up of side member anti cross member. The analysis is performed by finite element method. In order to be similar to real frame, modeled members are designed with shell elements. The static contact between the members and the rivets is considered at the joint, and the solution is compared with the simplified model without the rivet joints. The finite element code ABAQUS 6-2 is used for this analysis.

SUV 차량의 측면 충돌 안정성 최적화를 위한 측면 에어백 개발에 관한 연구

김용선(Yongsun Kim),박용재(Yongjae ParK),최형호(Hyeongho Choi),오창진(Changjin Oh),이창현(Changhyun Lee) 한국자동차공학회 2002 한국자동차공학회 춘 추계 학술대회 논문집 Vol.2002 No.5_3

Lately, Side airbag system has a tendency to be equipped as a standard for side crash performance in vehicle. Therefore, many studies on side airbag system, which is the important part of the safety system, have been proceeded in order to increase performance.<br/> In this study, the regulation of side impact, development procedure of side airbag and its tendency of technology are presented for competitive vehicle.