http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Kim, Yong-Guy,Lee, Jin-Hyung,Kim, Chang-Jin,Lee, Jae-Chan,Ju, Yoon Jung,Cho, Moo Hwan,Lee, Jintae Springer-Verlag 2012 Applied microbiology and biotechnology Vol.96 No.6
<P>Members of the actinomycetes family are a rich source of bioactive compounds including diverse antibiotics. This study sought to identify novel and non-toxic biofilm inhibitors from the actinomycetes library for reducing the biofilm formation of Pseudomonas aeruginosa PAO1. After the screening of 4104 actinomycetes strains, we found that the culture spent medium (1?%, v/v) of Streptomyces sp. BFI 230 and Kribbella sp. BFI 1562 inhibited P. aeruginosa biofilm formation by 90?% without affecting the growth of planktonic P. aeruginosa cells, while the spent media enhanced the swarming motility of P. aeruginosa. Global transcriptome analyses revealed that the spent medium of Streptomyces sp. BFI 230 induced expression of phenazine, pyoverdine, pyochelin synthesis genes, and iron uptake genes in P. aeruginosa. The addition of exogenous iron restored the biofilm formation and swarming motility of P. aeruginosa in the presence of the spent medium of Streptomyces sp. BFI 230, which suggests that the Streptomyces sp. BFI 230 strain interfered iron acquisition in P. aeruginosa. Experiments on solvent extraction, heat treatment, and proteinase K treatment suggested that hydrophilic compound(s), possibly extracellular peptides or proteins from Streptomyces sp. BFI 230 cause the biofilm reduction of P. aeruginosa. Together, this study indicates that actinomycetes strains have an ability to control the biofilm of P. aeruginosa.</P>
( Sung Guy Jin ),( Kyeong Soo Kim ),( Dong Wuk Kim ),( Dong Shik Kim ),( Youn Gee Seo ),( Toe Gyung Go ),( Yu Seok Youn ),( Jong Oh Kim ),( Chul Soon Yong ),( Han Gon Choi ) 영남대학교 약품개발연구소 2016 영남대학교 약품개발연구소 연구업적집 Vol.26 No.-
To develop a novel sodium fusidate-Ioaded triple polymer hydrogel dressing (TPHD). numerious polyvinyl alcohol-based (PVA) hydrogel dressings were prepared with various hydrophilic polymers using the freeze-thaw method. and their hydrogel dressing properties were assessed. Among the hydrophilic polymers tested, sodium alginate (SA) improved the swelling capacity the most, and polyvinyl pyrrolidone (PVP) provided the greatest improvement in bioadhesive stength and mechanical properties. Thus, PVA based-TPHDs were prepared using different ratios of PVP:SA The effect of selected PVP:SA ratios on the swelling capacity, bioadhesive strength, mechanical properties, and drug release, permeation and deposition characteristics of sodium fusidate-Ioaded PVA-based TPHDs were assessed. As the ratio of PVP:SA increased in PVA-Ioaded TPHD, the swelling capacity, mechanical properties, drug release. permeation and deposition were improved. The TPHD containing PVA. PVP, SA and sodium fusidate at the weight ratio of 10/6/1/1 showed excellent hydrogel dressing properties. release. permeation and deposition of drug. Within 24 h, 71.8 ± 1.3% of drug was released. It permeated 625.1 ±81.2μg/cm<sub>2</sub> through the skin and deposited of 313.8 ± 24.1 fLgfcm2 within 24 h. The results of in vivo pharmacodynamic studies showed that sodium fusidate-Ioaded TPHD was more effective in improving the repair process than was a commercial product. Thus, this sodium fusidate-Ioaded TPHD could be a novel tool in wound care.