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        Mitochondrial OXPHOS genes provides insights into genetics basis of hypoxia adaptation in anchialine cave shrimps

        Huayun Guo,Hao Yang,Yitao Tao,Dan Tang,Qiong Wu,Zhengfei Wang,Bo-Ping Tang 한국유전학회 2018 Genes & Genomics Vol.40 No.11

        Cave shrimps from the genera Typhlatya, Stygiocaris and Typhlopatsa (TST complex) comprises twenty cave-adapted taxa, which mainly occur in the anchialine environment. Anchialine habitats may undergo drastic environmental fluctuations, including spatial and temporal changes in salinity, temperature, and dissolved oxygen content. Previous studies of crustaceans from anchialine caves suggest that they have possessed morphological, behavioral, and physiological adaptations to cope with the extreme conditions, similar to other cave-dwelling crustaceans. However, the genetic basis has not been thoroughly explored in crustaceans from anchialine habitats, which can experience hypoxic regimes. To test whether the TST shrimpcomplex hypoxia adaptations matched adaptive evolution of mitochondrial OXPHOS genes. The 13 OXPHOS genes from mitochondrial genomes of 98 shrimps and 1 outgroup were examined. For each of these genes was investigated and compared to orthologous sequences using both gene (i.e. branch-site and Datamonkey) and protein (i.e. TreeSAAP) level approaches. Positive selection was detected in 11 of the 13 candidate genes, and the radical amino acid changes sites scattered throughout the entire TST complex phylogeny. Additionally, a series of parallel/convergent amino acid substitutions were identified in mitochondrial OXPHOS genes of TST complex shrimps, which reflect functional convergence or similar genetic mechanisms of cave adaptation. The extensive occurrence of positive selection is suggestive of their essential role in adaptation to hypoxic anchialine environment, and further implying that TST complex shrimps might have acquired a finely capacity for energy metabolism. These results provided some new insights into the genetic basis of anchialine hypoxia adaptation.

      • Behavior of hybrid CFST with FRP-confined UHPC core under axial compression

        Yi Tao,Jin-Ben Gu,Jian-Fei Chen,Peng Feng 국제구조공학회 2021 Steel and Composite Structures, An International J Vol.40 No.1

        A fiber-reinforced polymer (FRP)-confined concrete core that provides high strength and ductility under axial compression can act as strength enhancement in a hybrid column. In the present study, ordinary concrete was replaced with ultra-high-performance concrete (UHPC) to form an FRP-confined UHPC core (FCUC). The FCUC was embedded in square concrete-filled steel tube (CFST) columns to form a high-performance hybrid column (SCF-UHPC column for short). The axial compressive behavior of the SCF-UHPC was experimentally investigated using 12 SCF-UHPC columns and two ordinary CFST columns for comparison. The advantages of the SCF-UHPC include excellent axial load-bearing capacity, good ductility, and stable residual load-bearing capacity. The results show that failure of an SCF-UHPC column was caused by FRP rupture of FCUC, which occurred after steel tube buckling that results in the degraded stiffness. It was also shown that the load-displacement behavior of the SCF-UHPC composite column was determined by the UHPC core diameter and the corresponding confinement provided by the outer steel tube and inner FRP jacket. A hardening effect could be achieved when the confinement demand of the UHPC core was satisfied, whereas a plateau effect appeared if the confinement was insufficient. Furthermore, the load-bearing capacity and ductility of the SCF-UHPC columns improved with increased thickness of the steel tube and the FRP.

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