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        The dynamic transcriptome of waxy maize (Zea mays L. sinensis Kulesh) during seed development

        Wei Gu,Diansi Yu,Yuan Guan,Hui Wang,Tao Qin,Pingdong Sun,Yingxiong Hu,Jihui Wei,Hongjian Zheng 한국유전학회 2020 Genes & Genomics Vol.42 No.9

        Background Waxy maize (Zea mays L. sinensis Kulesh) is a mutant of maize (Zea mays L.) with a mutation at Waxy1 (Wx1) gene locus. The seed of waxy maize has higher viscosity compared to regular maize. By now, we know little about the expression patterns of genes that involved in the seed development of waxy maize. Objective By analyzing the transcriptome data during waxy maize seed development, we attempt to dig out the genes that may infuence the seed development of waxy maize. Methods The seeds of waxy maize inbred line SWL01 from six phases after pollination were used to do RNA-seq. Bioinformatics methods were used to analyze the expression patterns of the expressed genes, to identify the genes involved in waxy maize seed development. Results A total of 24,546 genes including 1611 transcription factors (TFs) were detected during waxy maize seed development. Coexpression analysis of expressed genes revealed the dynamic processes of waxy maize seed development. Particularly, 2457 genes including 177 TFs were specially expressed in waxy maize seed, some of which mainly involved in the process of seed dormancy and maturation. In addition, 2681, 5686, 4491, 4386, 3669 and 4624 genes were identifed to be diferential expressed genes (DEGs) at six phases compared to regular maize B73, and 113 DEGs among them may be key genes that lead the diference of seed development between waxy and regular maizes in milk stage. Conclusion In summary, we elucidated the expression patterns of expressed genes during waxy maize seed development globally. A series of genes that associated with seed development were identifed in our research, which may provide an important resource for functional study of waxy maize seed development to help molecular assisted breeding.

      • Analysis for Information Integration Platform on Warship

        Ying Xiong,Hu Jun Jie,Tang Di 보안공학연구지원센터 2016 International Journal of Grid and Distributed Comp Vol.9 No.1

        On the basis of network establishment, system architecture of warship’s information integral platform and XML distributed data exchange were researched. To focus on information system data and network resource on warship, the multilevel software architecture was adopted in simulation. On the data layer, circulated simulation data about manoeuvring, ship system, power and navigation were got. On the service layer, the software interface of all the simulated systems was supplied, which realized data collection and information storage. The last layer was for user by which human-computer interface was got through client software. To improve stability of ship’s local area network, double-ring topology was used as the main network, and very popular software named Wonderware in field of industry control was developed. With IAS platform, deployment of all the sub-stations was completed, and links between each collection and database were established. Multilevel computer network and information data integration frame on warship had many purpose, such as serving for oriented application system, data communication, information share and so on.

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