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Prevalence and Subtypes of Blastocystis in Alpacas, Vicugna pacos in Shanxi Province, China
Ye-Ting Ma,Qing Liu,Shi-Chen Xie,Xiao-Dong Li,Yuan-Yuan Ma,Tao-Shan Li,Wen-Wei Gao,Xing-Quan Zhu 대한기생충학ㆍ열대의학회 2020 The Korean Journal of Parasitology Vol.58 No.2
Blastocystis, an enteric protist, has been reported to be an important cause of protozoal gastrointestinal manifestations in humans and animals worldwide. Animals harboring certain Blastocystis subtypes (STs) may serve as a potential source of human infection. However, information about the prevalence and genetic diversity of Blastocystis in alpacas is limited. In the present study, a total of 366 fecal samples from alpacas in Shanxi Province, northern China, were examined for Blastocystis by PCR amplification of the small subunit rRNA gene, followed by sequencing and phylogenetic analysis. The prevalence of Blastocystis in alpacas was 23.8%, and gender difference in the prevalence of Blastocystis was observed. The most predominant Blastocystis ST was ST10, followed by ST14 and ST5. The detection of ST5, a potentially zoonotic genotype, indicates that alpacas harboring ST5 could be a potential source of human infection with Blastocystis. These data provide new insight into the prevalence and genetic diversity of Blastocystis in alpacas.
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Niu Ye,Pingping Zhang,Yifan Wang,Huiling Ma,Ting Zhang 한국원예학회 2021 Horticulture, Environment, and Biotechnology Vol.62 No.3
The green hull of fresh walnuts ( Juglans regia L.) is highly susceptible to browning and accompanied with deterioration ofthe walnut quality, and the currently available preservation technologies still do not meet the market threshold supply of freshwalnut. This study investigated the eff ect of controlled atmosphere (CA) storage alone and combination with 1-Methylcyclopropene(1-MCP) on hull browning and kernel quality of fresh in-hull walnut. Fresh in-hull walnuts were treated with air(Control), CA (5% O 2 , 7.5% CO 2 ), 1-MCP (0.5 μL L −1 ) + CA and stored at 0 ± 0.5 °C for 100 d. Browning incidence, hullredox metabolism, kernel color and walnut oil quality were analyzed. The results showed that CA promoted the activities ofsuperoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX), maintained total phenol (TP), γ-aminobutyricacid (GABA), ascorbic acid (ASA), and glutathione (GSH) contents, inhibited ethylene production, reduced reactive oxygenspecies (ROS) accumulation, and delayed the hull browning. Besides, CA attained the highest kernel quality throughinhibited the decrease of kernel color value and moisture content, and reduced acid value (AV) and peroxide value (POV)of walnut oil. While 1-MCP + CA treatment increased the activities of antioxidant enzymes, the combination weakened theother positive eff ects of CA. However, hull browning and kernel deterioration in 1-MCP + CA were less than that observedin air control. Overall, CA was an eff ective method for the preservation of fresh in-hull walnut at 0 ± 0.5 °C.
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