Reactive-extraction of 2,3-butanediol from fermentation broth by propionaldehyde: Equilibrium and kinetic study

Yanyang Wu,Yanjun Li,Jiawen Zhu,Jiaxian Liu 한국화학공학회 2013 Korean Journal of Chemical Engineering Vol.30 No.1

An effective process was developed to separate 2,3-butanediol (2,3-BD) from fermentation broth (FB)by reactive-extraction. Propionaldehyde (PRA) was used as reactant and reaction product 2-ethyl-4,5-dimethyl-1,3-dioxolane (EDD) acted as extractant. HCl was selected as catalyst. Appropriate conditions were obtained by experiment as follows: 10 oC, CHCl=0.2mol·L−1, two-stage cross-current extraction, reactant volume ratio (VPRA : VFB) for first stage and second stage is 0.10 and 0.05, respectively. The yield rate of 2,3-butanediol for the whole process can reach 90%w/w, and 2,3-butanediol in the final product can be more than 99% w/w. The novel process required less solution and especially had advantages in treating dilute fermentation broth. Furthermore, equilibrium and kinetic study were investigated on the reaction of propionaldehyde and 2,3-butanediol to provide basic data for process development. The results reveal that reaction enthalpy and activation energy of the reaction were −21.84±2.38 KJ·mol−1 and 51.97±2.84 KJ·mol−1, respectively. Kinetics was well described by pseudo-homogeneous model.

Reactive extraction of 2,3-butanediol from fermentation broth

Yanyang Wu,Yanjun Li,Jiawen Zhu,Jiaxian Liu 한국화학공학회 2013 Korean Journal of Chemical Engineering Vol.30 No.1

Biochemical 2,3-butanediol is a renewable material, but the lack of an effective separation process limits its industrial application. We developed an effective separation process to recover 2,3-butanediol from fermentation broth by reactive-extraction with ion-exchange resin HZ732 as catalyst. n-Butylaldehyde was used as both reactant and extractant. Feasible operation conditions were obtained as follows: room temperature, Ccat=200 g·L−1, three-stage cross-current extraction, with reactant ratio (VButylaldehyde : Vfermentation broth) 0.05 for each stage. Reactive-extraction can recover over 98% of 2,3-butanediol in the form of 2-propyl-4,5-dimethyl-1,3-dioxolane from fermentation broth. Then 2,3-butanediol was obtained by hydrolyzing 2-propyl-4,5-dimethyl-1,3-dioxolane and purified by vacuum distillation. The total yield rate of 2,3-butanediol through the process was over 94% and purity of final product reached 99%.

Separation of 2,3-Butanediol from Fermentation Broth by Reactiveextraction Using Acetaldehyde-cyclohexane System

Yanjun Li,Yanyang Wu,Jiawen Zhu,Jiaxian Liu 한국생물공학회 2012 Biotechnology and Bioprocess Engineering Vol.17 No.2

Biochemical 2,3-butanediol is a renewable material with the potential to be used as an alternative fuel. However, in the lack of an effective separation process has limited its industrial application. In this paper, an effective process was achieved to separate 2,3-butanediol by reactive-extraction. Acetaldehyde and cyclohexane were chosen as the reactant and extractant, respectively. Ion-exchange resin HZ732 was used as the catalyst. Reaction equilibrium and a kinetic study on the reaction between 2,3-butanediol and acetaldehyde were investigated to provide basic data for process development. The reaction enthalpy and activation energy of reaction of 2,3-butanediol and acetaldehyde were −30.05 ± 1.62 KJ/mol and 45.29 ± 2.89 KJ/mol,respectively. Feasible conditions were obtained as follows:operating temperature = 20℃, acetaldehyde: 2,3-butanediol = 0.5:1 (w/w), cyclohexane: fermentation broth = 0.5:1 (w/w), catalyst amount = 100 g/L, stirring rate = 500 rpm and three-stage counter-current extraction method was used. Under these conditions, the total yield rate of 2,3-butanediol from fermentation broth was over 90% and the mass fraction of 2,3-butanediol in the final product reached 99%. Biochemical 2,3-butanediol is a renewable material with the potential to be used as an alternative fuel. However, in the lack of an effective separation process has limited its industrial application. In this paper, an effective process was achieved to separate 2,3-butanediol by reactive-extraction. Acetaldehyde and cyclohexane were chosen as the reactant and extractant, respectively. Ion-exchange resin HZ732 was used as the catalyst. Reaction equilibrium and a kinetic study on the reaction between 2,3-butanediol and acetaldehyde were investigated to provide basic data for process development. The reaction enthalpy and activation energy of reaction of 2,3-butanediol and acetaldehyde were −30.05 ± 1.62 KJ/mol and 45.29 ± 2.89 KJ/mol,respectively. Feasible conditions were obtained as follows:operating temperature = 20℃, acetaldehyde: 2,3-butanediol = 0.5:1 (w/w), cyclohexane: fermentation broth = 0.5:1 (w/w), catalyst amount = 100 g/L, stirring rate = 500 rpm and three-stage counter-current extraction method was used. Under these conditions, the total yield rate of 2,3-butanediol from fermentation broth was over 90% and the mass fraction of 2,3-butanediol in the final product reached 99%.

Experimental study on the compression of concrete filled steel tubular latticed columns with variable cross section

Yan Yang,Jun Zhou,Jiangang Wei,Lei Huang,Qingxiong Wu,Bao-chun Chen 국제구조공학회 2016 Steel and Composite Structures, An International J Vol.22 No.3

The effects of slenderness ratio, eccentricity and column slope on the load-carrying capacities and failure modes of variable and uniform concrete filled steel tubular (CFST) latticed columns under axial and eccentric compression were investigated and compared in this study. The results clearly show that all the CFST latticed columns with variable cross section exhibit an overall failure, which is similar to that of CFST latticed columns with a uniform cross section. The load-carrying capacity decreases with the increase of the slenderness ratio or the eccentricity. For 2-m specimens with a slenderness ratio of 9, the ultimate load-carrying capacity is increased by 3% and 5% for variable CFST latticed columns with a slope of 1:40 and 1:20 as compared with that of uniform CFST latticed columns, respectively. For the eccentrically compressed variable CFST latticed columns, the strain of the columns at the loading side, as well as the difference in the strain, increases from the bottom to the cap, and a more significant increase in strain is observed in the cross section closer to the column cap.

Effects of compound traditional Astragalus and Salvia Miltiorrhiza extract on acute and chronic hepatic injury

Yan Yang,Xiaoxiang Zhang,Xin Liu,Chao Wu,Min-Zhu Chen 셀메드 세포교정의약학회 2013 셀메드 (CellMed) Vol.3 No.2

Previous reports showed that Compound Astragalus and Salvia miltiorrhiza extract (CASE), which was mainly composed of astragalosides, astragalus polysaccharide and salvianolic acids, inhibited hepatic fibrosis by mediating transforming growth factor-β (TGF-β)/Smad signaling. Our aim was to examine the effects of CASE on D-galactosamine (D-GalN) treated liver injury in mice and carbon tetrachloride (CCl4)-induced liver fibrosis in rats. CASE was administered to mice with D-GalN-induced liver injury and to rats with CCl4-induced liver fibrosis, respectively. Liver injury was routinely evaluated by relative liver weight, serum levels of ALT, AST, hyaluronic acid (HA), hepatic malondialdehyde (MDA) content, superoxide dismutase (SOD) activity, hydroxyproline (HYP) and histopathologic changes. Treatment of mice with CASE (60, 120, and 240 mg/kg, ig) significantly lowered ALT, relative liver weight, and MDA levels when compared with D-GalN treated mice. CASE (120, 240 mg/kg) significantly lowered ALT, AST, HA, HYP, and MDA levels against CCl4 treated rats. Decreased SOD level was reversed with CASE treatment. Upon histopathological examination, CASE treatment had significantly inhibitory effect on the progression of hepatic fibrosis in rats. These results indicate that CASE might be effective in treatment and prevention of acute and chronic hepatic injury due to its antioxidant activity.