The Contemporary Value of Xunzi's Political Ethics Thought for the Social Administration

Dai Yanjun,Su Lin 한국윤리학회 2014 倫理硏究 Vol.95 No.1

Xunzi’s political ethical thought, including gentleman personality, people-oriented, prospering etiquettes and respecting law and so on, still has a relational meaning for the modern social political moral construction. Gentleman personality comes with an obligation to improve the leading officials’ morality; the idea of people-oriented has a vital significance for establishing“ put people first“ thought throughout society and insisting the mass line; This statecraft that focuses both on ritual and law has a very important revelation for improving the foundation for anti-corruption and a clean government. Xunzi’s political ethical thought provides the value for our country’s conducting a harmonious and efficient social administration through the three dimensions mentioned above.

Accurate Voltage Parameter Estimation for Grid Synchronization in Single-Phase Power Systems

Dai, Zhiyong,Lin, Hui,Tian, Yanjun,Yao, Wenli,Yin, Hang The Korean Institute of Power Electronics 2016 JOURNAL OF POWER ELECTRONICS Vol.16 No.3

This paper presents an adaptive observer-based approach to estimate voltage parameters, including frequency, amplitude, and phase angle, for single-phase power systems. In contrast to most existing estimation methods of grid voltage parameters, in this study, grid voltage is treated as a dynamic system related to an unknown grid frequency. Based on adaptive observer theory, a full-order adaptive observer is proposed to estimate voltage parameters. A Lyapunov function-based argument is employed to ensure that the proposed estimation method of voltage parameters has zero steady-state error, even when frequency varies or phase angle jumps significantly. Meanwhile, a reduced-order adaptive observer is designed as the simplified version of the proposed full-order observer. Compared with the frequency-adaptive virtual flux estimation, the proposed adaptive observers exhibit better dynamic response to track the actual grid voltage frequency, amplitude, and phase angle. Simulations and experiments have been conducted to validate the effectiveness of the proposed observers.

Antibacterial activity of antimicrobial peptide gcDefb1 against foodborne pathogenic bacteria and its application in pork storage

Bin Dong,Yanjun Lin,Jun Wang,Wen Du,Chunlong Sun,Tao Wu,Shijun Fu 한국식품과학회 2022 Food Science and Biotechnology Vol.31 No.5

Pork and its products are preferably contaminated by bacteria; thus, it is essential to develop low-cost, high-efficiency and biologically safe preservatives to prevent the growth of bacteria during storage. In the current study, grass carp β-defensin 1 (gcDefb1) was produced and purified from Pichia pastoris through the heterologous expression method. The in vitro antimicrobial assay demonstrated that yeast-derived gcDefb1 possesses a broad antibacterial spectrum, including both Gram-positive and -negative bacteria, and the MIC values against Escherichia coli ATCC 25,922 were as low as 30 μg/mL and showed no cytotoxicity or hemolytic activity. The bactericidal rate of gcDefb1 was less than 60 min by disrupting the cell membranes, and it inhibited the formation of bacterial biofilms. Moreover, gcDefb1 was used as a biopreservative for pork storage, indicating that the physicochemical and sensory qualities were improved. This study provides an efficient method to prepare and utilize gcDefb1 as a novel biopreservative.

Recombinant human β-defensin130 inhibited the growth of foodborne bacteria through membrane disruption and exerted anti-inflammatory activity

Bin Dong,Yanjun Lin,Zhiwei Su,Chunlong Sun,Jun Wang,Shijun Fu,Wen Du,Tao Wu 한국식품과학회 2022 Food Science and Biotechnology Vol.31 No.7

Foodborne pathogens causing food poisoning and infections are detrimental to human health, and the abuse of antibiotics induced severe antibiotic resistance in past decades. Thus, it is urgent to develop new antimicrobial agents. In the current study, human β-defensin 130 (hBD130), which is an antimicrobial peptide identified in human macrophages in 2017, was initially produced in Pichia pastoris. The purified hBD130 demonstrated broad bactericidal spectrum against foodborne pathogens through a membrane disruption, with concentrations ranging from 10 to 45 μg/mL. Moreover, hBD130 showed a low hemolytic effect and nearly no cytotoxicity to mammalian cells with a dosage of 400 μg/mL. In addition, the secretion amounts and mRNA levels of NO, IL-6, IL-1β, and TNF-α in LPS-induced mouse macrophage were significantly decreased with 1 mg/mL of hBD130. Taken together, these results showed that hBD130 is a promising antimicrobial agent to treat foodborne bacterial infections and inflammation.

Accurate Voltage Parameter Estimation for Grid Synchronization in Single-Phase Power Systems

Zhiyong Dai,Hui Lin,Yanjun Tian,Wenli Yao,Hang Yin 전력전자학회 2016 JOURNAL OF POWER ELECTRONICS Vol.16 No.3

This paper presents an adaptive observer-based approach to estimate voltage parameters, including frequency, amplitude, and phase angle, for single-phase power systems. In contrast to most existing estimation methods of grid voltage parameters, in this study, grid voltage is treated as a dynamic system related to an unknown grid frequency. Based on adaptive observer theory, a full-order adaptive observer is proposed to estimate voltage parameters. A Lyapunov function-based argument is employed to ensure that the proposed estimation method of voltage parameters has zero steady-state error, even when frequency varies or phase angle jumps significantly. Meanwhile, a reduced-order adaptive observer is designed as the simplified version of the proposed full-order observer. Compared with the frequency-adaptive virtual flux estimation, the proposed adaptive observers exhibit better dynamic response to track the actual grid voltage frequency, amplitude, and phase angle. Simulations and experiments have been conducted to validate the effectiveness of the proposed observers.

Antimicrobial property of recombinant Lactolisterin BU in vitro and its initial application in pork refrigerated storage

Dong Bin,Zhou Guowen,Lin Yanjun,Yu Cailing,Wang Jun,Sun Chunlong,Wu Tao 한국응용생명화학회 2021 Applied Biological Chemistry (Appl Biol Chem) Vol.64 No.5

Lactolisterin BU is a novel bacteriocin identified from Lactococcus lactis in 2017. It exhibits antimicrobial activity against food spoilage and foodborne pathogens. In this study, Lactolisterin BU was expressed in Pichia pastoris (P. pastoris) and isolated from the supernatant of yeast culture for the first time. It was found to exhibit a broad antimicrobial spectrum and rapid bactericidal activity against foodborne bacterial pathogens, both gram-positive and gramnegative ones, with minimum inhibition concentrations ranging within 10–60 μg/mL. The recombinant Lactolisterin BU (rLactolisterin BU) also had an antioxidant effect and was resistant to heating, acid–base, and high-dose-saline treatments and barely had any hemolytic activity or cytotoxicity. Moreover, rLactolisterin BU effectively suppressed the growth of bacterial pathogens; suppressed the increases in pH, total volatile basic nitrogen (TVB-N), and thiobarbituric acid reactive substances (TBARS) of pork samples; and maintained a high quality of fresh pork during storage at 4 ℃. Furthermore, rLactolisterin BU effectively inhibited the growth of three kinds of bacteria in a pork-spoilage model. Taken together, rLactolisterin BU could be a promising preservative for food storage.

Multivariate analysis of original identification and chemical markers exploration of Chinese ginger

Mengqing Guo,Qian Shen,Yu Wu,Lili Li,Lin Zhang,Yu Wang,Yanjun Zhang,Pengwei Zhuang,Hong Guo 한국식품과학회 2023 Food Science and Biotechnology Vol.32 No.7

Ginger (Zingiber officinale) is one of the most widely consumed dietary supplements. However, the content of active ingredients varied greatly from place to place. In this study, we first identified and compared the compositions of ginger samples from six different origins. Then, we evaluated the anti-inflammatory activity of different samples in LPS-stimulated RAW264.7 cells. The results indicated that highly variable in chemical composition and activity for ginger from different origin. Further, correlation analysis showed that isoborneol, terpineol, α-curcumene, germacrene D, α-elemol and 8-shogaol exhibited a strong correlation with inflammatory factors, which could be used as potential chemical markers to evaluate quality and distinguish source of ginger. Finally, comprehensive evaluation found that the ginger from Sichuan exerts stronger anti-inflammatory properties. This study will help to select ginger varieties with excellent characteristics, provide theoretical basis for the development and utilization of ginger.