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        Ciceribacter ferrooxidans sp. nov., a nitrate-reducing Fe(II)-oxidizing bacterium isolated from ferrous ion-rich sediment

        Tongchu Deng,Youfen Qian,Xingjuan Chen,Xunan Yang,Jun Guo,Guoping Sun,Meiying Xu 한국미생물학회 2020 The journal of microbiology Vol.58 No.5

        A nitrate-reducing Fe(II)-oxidizing bacterial strain, F8825T, was isolated from the Fe(II)-rich sediment of an urban creek in Pearl River Delta, China. The strain was Gram-negative, facultative chemolithotrophic, facultative anaerobic, nonspore- forming, and rod-shaped with a single flagellum. Phylogenetic analysis based on 16S rRNA gene sequencing indicated that it belongs to the genus Ciceribacter and is most closely related to C. lividus MSSRFBL1T (99.4%), followed by C. thiooxidans F43bT (98.8%) and C. azotifigens A.slu09T (98.0%). Fatty acid, polar lipid, respiratory quinone, and DNA G + C content analyses supported its classification in the genus Ciceribacter. Multilocus sequence analysis of concatenated 16S rRNA, atpD, glnII, gyrB, recA, and thrC suggested that the isolate was a novel species. DNA–DNA hybridization and genome sequence comparisons (90.88 and 89.86%, for values of ANIm and ANIb between strains F8825T with MSSRFBL1T, respectively) confirmed that strain F8825T was a novel species, different from C. lividus MSSRFBL1T, C. thiooxidans F43bT, and C. azotifigens A.slu09T. The physiological and biochemical properties of the strain, such as carbon source utilization, nitrate reduction, and ferrous ion oxidation, further supported that this is a novel species. Based on the polyphasic taxonomic results, strain F8825T was identified as a novel species in the genus Ciceribacter, for which the name Ciceribacter ferrooxidans sp. nov. is proposed. The type strain is F8825T (= CCTCC AB 2018196T = KCTC 62948T).

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        Solvothermal Synthesis of Novel 3D Peony-Like TiO2 Microstructures and its Enhanced Photocatalytic Activity

        Yugan He,Qi Yan,Xiaoyu Chang,Meiying Zhu,Weiwei Wang,Menglong Dong,Lianjie Song,Junjiao Yang,Yaodong Huang 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2018 NANO Vol.13 No.5

        A TiO2 photocatalyst with peony-like microstructures and a large percentage of exposed {001} facets was synthesized using a facile solvethermal method. The peony-like TiO2 was obtained using HF as a capping agent, TiCl4 as the precursor and ethanol as the solvothermal agent. The parameters which influence the mophology and formation mechanism of the products including the HF concentration, the reaction time and temperature and the solvothermal solvent, were investigated. The samples were characterized using field emission scanning electron microscopy, transmission electron microscopy, X-ray diffraction and N2 adsorption and desorption analysis. As the reaction time or reaction temperature increased, the morphology TiO2 changed from hexagonally assembled microspheres to peony-like microflowers which were composed of stacks of ultrathin nanosheets. The other reaction parameters also play a crucial role in the formation of the TiO2 microstuctures. Photocatalytic experiments showed that the synthesized TiO2 out-performed Degussa P25 in the photodegradation of methelene blue under a very weak UV light irradiation (power: 8 W, light intensity: 0.4mW cm -1).

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        Highly elastic aerogel derived from spent coffee grounds as oil removal adsorbent

        Yongli Chen,Weijie Cai,Meng Zhang,Meiying Xie,Fengzhi Tan,Fan Yang 한국화학공학회 2022 Korean Journal of Chemical Engineering Vol.39 No.6

        In the face of increasing environmental pollution, aerogels have emerged as valuable materials for potentialoil/water separation. However, many of the currently developed aerogels have unsatisfactory compressibility, high costand a single hydrophobic modification method, which limits larger-scale application. In this work, a type of aerogelwith compressible, inexpensive, and fully biodegradable features was designed via a novel zirconium chloride modificationstrategy. Typically, a series of aerogels (HCSW-1, HCSW-2, and HCSW-3) were readily prepared from a mixture ofspent coffee grounds, waste paper and sodium alginate. The prepared aerogels exhibited good elasticity, low density(0.024 g cm3), high porosity (98.3%), efficient oil/water separation and good oil uptake (23-44 times of its weight). Inaddition, the as-prepared aerogels can be easily recycled several times, thus meeting the demand of actual oil/waterseparation. Such prominent results provide a new perspective for the development of efficient hydrophobic aerogels inthe treatment of offshore oil spills and industrial wastewater.

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        Effects of Continuous Straw Returning on Soil Functional Microorganisms and Microbial Communities

        Guan Yunpeng,Wu Meikang,Che Songhao,Yuan Shuai,Yang Xue,Li Siyuan,Tian Ping,Wu Lei,Yang Meiying,Wu Zhihai 한국미생물학회 2023 The journal of microbiology Vol.61 No.1

        This study examined the changes in soil enzymatic activity, microbial carbon source metabolic diversity, and straw decomposition rates in paddy fields treated with 1, 2, or 3 years of straw returning (SR1–SR3). The soil’s ability to decompose straw and cellulolytic bacteria increased with the number of treatment years (1: 31.9% vs. 2: 43.9% vs. 3: 51.9%, P < 0.05). The numbers of Azotobacter, Nitrobacteria, cellulolytic bacteria, and inorganic phosphate bacteria increased progressively with the numbers of straw returning years. Cellulolytic bacteria and inorganic phosphate bacteria were significantly positively correlated with the decomposition rate (r = 0.783 and r = 0.375, P < 0.05). Based on 16S sequencing results, straw returning improved the microbial diversity of paddy soils by increasing unclassified bacteria and keeping dominant soil microorganism populations unchanged. The relative importance of individual microbial taxa was compared using random forest models. Proteobacteria, ammoniating bacteria, and potassium dissolving bacteria contributed to peroxidase activity. The significant contributors to phosphate monoesterase were Acidobacteriota, Desulfobacterota, ammoniating bacteria, cellulolytic bacteria, and potassium-dissolving bacteria. Proteobacteria, ammoniating bacteria, cellulolytic bacteria, and potassium-dissolving bacteria contributed to urease activity. Desulfobacterota, ammoniating bacteria, cellulolytic bacteria, and potassium-dissolving bacteria contributed to the neutral invertase activity. In conclusion, soil microbial community structure and function were affected within 2 years of straw returning, which was driven by the combined effects of soil organic carbon, available nitrogen, available potassium, and pH. With elapsing straw returning years, soil properties interacted with soil microbial communities, and a healthier soil micro-ecological environment would form.

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