Comprehensive transcriptome analysis discovers novel candidate genes related to leaf color in a Lagerstroemia indica yellow leaf mutant

Ya Li,Zhenyu Zhang,Peng Wang,Shu’an Wang,Lingling Ma,Linfang Li,Rutong Yang,Yuzhu Ma,Qing Wang 한국유전학회 2015 Genes & Genomics Vol.37 No.10

Lagerstroemia indica is a popular woody ornamental plant throughout the world. However, relatively little is known about the molecular processes regulating leaf color in L. indica compared with other ornamental plants. Although yellow leaf mutants from various organisms have been well characterized, L. indica yellow leaf mutant has not yet been reported. In this study, a L. indica yellow leaf mutant, named YL03, was characterized and its leaf transcriptome was sequenced. A total of 30,712,752 reads were generated and assembled de novo into 45,308 unigenes with an average length of 987.51 bp. Among these unigenes, 21,339 (47.10 %) were identified as putative homologs of annotated sequences in public databases. A total of 79 unigenes involved in chlorophyll biosynthesis and degradation, photosynthesis and chloroplast development were identified. The expression levels of those genes were detected using quantitative real-time PCR in this study. Among those genes, 11 unigenes showed highly significant difference in the mutant compared to wild type plants. Conclusively, the leaf color formation is greatly affected by the activity of chloroplast development and chlorophyll metabolism. And the possible formation pathway of yellow leaf mutant is deduced based on our results.

Immobilization of Candida sp.99-125 lipase onto silanized SBA-15 mesoporous materials by physical adsorption

Ya Li,Pingfang Han,Wei Wang 한국화학공학회 2014 Korean Journal of Chemical Engineering Vol.31 No.1

SBA-15-NH2 and SBA-15-Cl Mesoporous Materials were prepared by modifying SBA-15 with silane coupling agent 3-aminopropyltriethoxysilane and 3-chloropropyltriethoxysilane using the post-synthesis method. The mesoporous samples were characterized by Fourier transform infrared spectra and nitrogen adsorption. Compared with SBA-15-NH2, SBA-15-Cl has suitable pore opening for further utilization in the immobilization of Candida sp.99-125 lipase by physical adsorption. The influences of lipase concentration and immobilizing time on the immobilization efficiency were investigated. Meanwhile, the lipase immobilized on SBA-15-Cl showed higher thermal, pH and storage stability than that of free lipase. Further study demonstrated that lipase immobilized on SBA-15-Cl could be used eight times without significant decrease of enzyme activity. The phenomenon was associated with the shrinkage of the pore opening of SBA-15-Cl keeping lipase from leaping out.

Effects of Reaction Parameters on Self-degradation of L-ascorbic Acid and Self-degradation Kinetics

Ya Li,Yan Yang,Ai-Nong Yu,Kui Wang 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.1

The degradation behavior of L-ascorbic acid (ASA) was investigated under different parameters of temperature, time, and pH. Higher temperatures and longer times accelerated the ASA degradation. Degradation product distributions changed with different pH values. As solution pH of 4.5 was beneficial for formation of uncolored intermediate products with an absorbance maximum at 294 nm. Formation of brown products was promoted at pH values from 5.8 to 6.8 with an absorbance maximum at 420 nm. Under different pH conditions, volatile products formation varied. Furfural and derivatives of furan were primary products due to the effects of pH. The non-enzymatic selfdegradation behavior of ASA was characteristic of first-order kinetics based on a classic dynamic model. Activation energy values varied under different pH values. An ASA degradation mechanism and pathway are proposed.

Preparation, Structure and Performance of Poly(lactic acid)/Poly(lactic acid)-γ -Cyclodextrin Inclusion Complex-Poly(glycidyl methacrylate) Composites

Ya Li,Weijun Zhen 한국고분자학회 2018 Macromolecular Research Vol.26 No.3

Poly(lactic acid)-γ-cyclodextrin inclusion complex-poly(glycidyl methacrylate) (PLA-IC-PGMA) was synthesized with poly(lactic acid) (PLA) and γ-cyclodextrin inclusion complex via surface initiated atom transfer radical polymerization. Then, the PLA/PLA-IC-PGMA composite was prepared by melt blending using PLA-IC-PGMA as additive. Structure and properties of PLA-IC-PGMA and PLA/PLA-IC-PGMA composites were confirmed by X-ray diffraction patterns, 1H nuclear magnetic resonance spectroscopy, Fourier transform infrared spectra, X-ray photoelectron spectroscopy, thermogravimetric analysis, differential scanning calorimetry (DSC), polarized optical microscopy, rheological analysis, etc. Mechanical testing analysis showed that the tensile strength, elongation at break, and impact strength of PLA/ (1 wt%) PLAIC- PGMA composites were improved by 2.3%, 10.8%, and 52.5%, respectively, compared with pure PLA. Scanning electron microscopy analysis demonstrated that the toughness of PLA/PLA-IC-PGMA composite was improved. The results of DSC and TG showed that the cold crystallization peak of PLA composites after the addition of PLA-IC-PGMA disappeared, and its crystallinity was increased by 25% compared with PLA. Furthermore, the thermal stability of PLA/PLA-IC-PGMA composite was improved. Dynamic mechanical analysis showed that the glass transition temperature of PLA/PLA-IC-PGMA composites decreased by 8 oC, which indicated that the flexibility of the chain of PLA was greatly improved. Therefore, the addition of PLA-IC-PGMA improved the comprehensive performance of PLA.

A Comparison of Eu-Doped -Fe2O3 Nanotubes and Nanowires for Acetone Sensing

Yali Cheng,Yifang Wang,Jinbao Zhang,Haiying Li,Li Liu,Yu Lina,Liting Du,Haojie Duan 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2017 NANO Vol.12 No.11

Pure and Eu-doped (1.0, 3.0, 5.0 wt.%) α-Fe2O3 (PFO and EFO) nanotubes and nanowires have been successfully synthesized through the combination of electrospinning and calcination techniques. The structures, morphologies and chemical compositions of the as-obtained products were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), thermogravimetric and differential scanning calorimetry (TG-DSC) and energy dispersive spectrum (EDS), respectively. To demonstrate the superior gas sensing performance of the doped nanotubes, a contrastive gas sensing study between PFO (EFO) nanotubes and nanowires was performed. It turned out that Eu doping could magnify the impact of morphology on gas sensitivity. Specifically, at the optimum operating temperature of 240 ℃, the response value of PFO nanotubes to 100 ppm acetone is slightly higher than that of nanowires (3.59/2.20). EFO (3.0 wt.%) nanotubes have a response of 84.05, which is almost 2.7 times as high as that of nanowires (31.54). Moreover, they possess more rapid response/recovery time (11 s and 36 s, respectively) than nanowires (17 s and 40 s, respectively). The lowest detection limit for acetone is 0.1 ppm and its response is 2.15. In addition, both of EFO nanotubes and nanowires sensors have a good linearity (0.1–500 ppm) and favorable selectivity in acetone detection.

Perturbed three-step iterative processes witherrors for general strongly nonlinear quasivariationalinequalities

Yali Zhao,Zeqing Liu,Shin Min Kang,Zunquan Xia 한국전산응용수학회 2005 Journal of applied mathematics & informatics Vol.17 No.1-2

In this paper, we introduce and study a class of general strongly nonlinear quasivariational inequalities in Hilbert spaces. We prove the existence and uniqueness of solution and convergence of the perturbed the three-step iterative sequences with errors for this kind of general strongly nonlinear quasivariational inquality problems involving relaxed Lipschitz, relaxed monotone, and strongly monotone mappings. Our results extend, improve, and unify many known results due to Liu-Ume-Kang, Kim-Kyung, Zeng and others

Effect of Radio Frequency Sterilization on the Pre-Packaged Food

Yali Tang 한국산업식품공학회 2016 학술대회 및 심포지엄 Vol.2016 No.10

Low water activity foods usually do not support growth of pathogens. However, in recent years, a number of outbreaks associated with low water activity foods occurred, making people have a new understanding of microorganisms about low water activity foods and resulting in the safety of these foods becoming a major concern. Traditional thermal sterilization methods are difficult to be applied in low water activity foods because of its poor heat transfer and strong heat resistance of microorganisms. Radio frequency (RF) is considered as one of the most potential technologies in food industry and has an excellent prospect on content of the advantages of rapid and uniform heating. The Principle of RF-Sterilization is similar to Microwave, but RF has lower frequency and wider wavelength, therefore RF penetration depth is more suitable for Sterilization. For the pre-packaged food, there is few report on the effect of RF on the food and packaging materials. This presentation aims to discuss the influence of RF through comparing to Microwave.

Effect of Radio Frequency Sterilization on the Pre-Packaged Food

Yali Tang 한국산업식품공학회 2016 식품과 기계 Vol.13 No.1

Modeling dynamic behavior of MDOF systems with multiple bilinear springs

Yali Ma,Shudong Yu,Delun Wang 대한기계학회 2018 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.32 No.9

A computational scheme is presented in this paper to simulate dynamical behavior of multiple degrees of freedom (MDOF) systems with multiple bilinear springs. In the proposed scheme, a bilinear spring is modeled using by two parallel springs - a primary spring and a secondary spring. The primary spring is an ordinary linear spring having identical stiffness in tension and compression, and is active for tension and compression. The secondary spring, whose stiffness characterizes the bilinearity, is active only during compression. It is employed in connection with the Newmark integration method and the linear complementarity problem (LCP) formulation to obtain time-domain responses of dynamical systems with bilinear springs due to initial disturbances and harmonic excitations. The scheme described in this paper is effective in dealing with the sudden transition from tension to compression and vice versa simultaneously for all bilinear springs. Numerical results for bilinear oscillators with finite bilinearity ratios and impact oscillators with an infinite bilinearity ratio show that the proposed bilinear spring model is accurate, generic and valid for bilinearity ratios ranging from zero to infinity. Orderly and chaotic behavior of viscously damped 3-DOF system under harmonic excitation is studied for a wide range of excitation frequencies and bilinear ratios to demonstrate the effectiveness and applicability of the proposed model for MDOF bilinear systems.

Epidemiology and resistance features of Acinetobacter baumannii isolates from the ward environment and patients in the burn ICU of a Chinese hospital

Yali Gong,Xiaodong Shen,Guangtao Huang,Cheng Zhang,Xiaoqiang Luo,Supeng Yin,Jing Wang,Fuquan Hu,Yizhi Peng,Ming Li 한국미생물학회 2016 The journal of microbiology Vol.54 No.8

Acinetobacter baumannii is an important opportunistic pathogen that causes severe nosocomial infections, especially in intensive care units (ICUs). Over the past decades, an everincreasing number of hospital outbreaks caused by A. baumannii have been reported worldwide. However, little attention has been directed toward the relationship between A. baumannii isolates from the ward environment and patients in the burn ICU. In this study, 88 A. baumannii isolates (26 from the ward environment and 62 from patients) were collected from the burn ICU of the Southwest Hospital in Chongqing, China, from July through December 2013. Antimicrobial susceptibility testing results showed that drug resistance was more severe in isolates from patients than from the ward environment, with all of the patient isolates being fully resistant to 10 out of 19 antimicrobials tested. Isolations from both the ward environment and patients possessed the β-lactamase genes blaOXA-51, blaOXA-23, blaAmpC, blaVIM, and blaPER. Using pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST), these isolates could be clustered into 4 major PFGE types and 4 main sequence types (ST368, ST369, ST195, and ST191) among which, ST368 was the dominant genotype. Epidemiologic and molecular typing data also revealed that a small-scale outbreak of A. baumannii infection was underway in the burn ICU of our hospital during the sampling period. These results suggest that dissemination of β-lactamase genes in the burn ICU might be closely associated with the high-level resistance of A. baumannii, and the ICU environment places these patients at a high risk for nosocomial infection. Cross-contamination should be an important concern in clinical activities to reduce hospital acquired infections caused by A. baumannii.