Distributed control system architecture for deep submergence rescue vehicles

Yushan Sun,Xiangrui Ran,Guocheng Zhang,Fanyu Wu,Chengrong Du 대한조선학회 2019 International Journal of Naval Architecture and Oc Vol.11 No.1

The control architectures of Chuan Suo (CS) deep submergence rescue vehicle are introduced. The hardware and software architectures are also discussed. The hardware part adopts a distributed control system composed of surface and underwater nodes. A computer is used as a surface control machine. Underwater equipment is based on a multi-board-embedded industrial computer with PC104 BUS, which contains IO, A/D, D/A, eight-channel serial, and power boards. The hardware and software parts complete data transmission through optical fibers. The software part involves an IPC of embedded Vxworks real-time operating system, upon which the operation of I/O, A/D, and D/A boards and serial ports is based on; this setup improves the real-time manipulation. The information flow is controlled by the software part, and the thrust distribution is introduced. A submergence vehicle heeling control method based on ballast water tank regulation is introduced to meet the special heeling requirements of the submergence rescue vehicle during docking. Finally, the feasibility and reliability of the entire system are verified by a pool test.

CpG coupled N-glycosylation mutant of HCV E1-E2 as a potential therapeutic DNA vaccine with enhanced cellular and neutralizing immune responses

Yushan Ren,Miao Lin,Yuanqin Min,Xiao-Lian Zhang 한국당과학회 2012 한국당과학회 학술대회 Vol.2012 No.1

N-linked glycosylation of viral proteins have been implicated in shielding antigen epitopes and blocking neutralizing antibodies production and contribute to the evasion of HCV from the host immune response. In this study, the effects of N-linked glycosylation of Hepatitis C virus (HCV) envelope E1 and E2 proteins, the naturally poor immunogens, on the induction of specific immune response were examined. We constructed plasmids containing the genes encoding both wild type E1E2 proteins and mutated E1E2 proteins in which N-linked glycosylation sites are mutated individually or in combination by site-directed mutagenesis or coupled with CpG, a TLR9 ligand. The immunoreactivity of wild type E1E2 and mutated E1E2 proteins or coupled with immune activator CpG was analyzed in BALB/C mice using a DNA-based vaccination approach. We found that E1E2 specific N-glycosylated mutant induced much higher IgG titer than wild type (WT) E1E2. E1E2 mutant vaccinated mice developed a mixture of IgG1 and IgG2a, but CpG-E1E2 mutant and E1-E2 wild type dominantly developed IgG2a isotype. And CpG-E1E2 mutant significantly stimulated Th1-type response and specific CD8+T cells cytotoxic T lymphocytes (CTL) activities. More important we found that CpG-E1E2 mutant significantly induced much higher neutalizing antibody than E1-E2-WT and had the highest level of neutralizing antibodies among all groups. The monoclonal clonal antibody (McAb) were prepared from CpG-E1E2 mutant immunized mice and the main neutralizing McAb 1C2 had significantly neutralizing Ab activity with blocking HCVcc infection in Huh7.5.1 cells in vitro. Epitope mapping of binding of a neutralizing McAb 1C2 was also analyzed. Our data indicate that the presence of glycans at the surface of HCV envelope proteins could help explain how HCV evades the humoral immune response and why most HCV infections lead to chronicity. CpG have the ability to augment both neutralizing antibody and cellular responses of E1E2 mutant. The CpG-E1E2 mutant holds potential applicant for the development of therapeutic vaccine with enhanced cellular and neutralizing immune responses.

Distributed control system architecture for deep submergence rescue vehicles

Sun, Yushan,Ran, Xiangrui,Zhang, Guocheng,Wu, Fanyu,Du, Chengrong The Society of Naval Architects of Korea 2019 International Journal of Naval Architecture and Oc Vol.11 No.1

The control architectures of Chuan Suo (CS) deep submergence rescue vehicle are introduced. The hardware and software architectures are also discussed. The hardware part adopts a distributed control system composed of surface and underwater nodes. A computer is used as a surface control machine. Underwater equipment is based on a multi-board-embedded industrial computer with PC104 BUS, which contains IO, A/D, D/A, eight-channel serial, and power boards. The hardware and software parts complete data transmission through optical fibers. The software part involves an IPC of embedded Vxworks real-time operating system, upon which the operation of I/O, A/D, and D/A boards and serial ports is based on; this setup improves the real-time manipulation. The information flow is controlled by the software part, and the thrust distribution is introduced. A submergence vehicle heeling control method based on ballast water tank regulation is introduced to meet the special heeling requirements of the submergence rescue vehicle during docking. Finally, the feasibility and reliability of the entire system are verified by a pool test.

A COST-EFFECTIVE PROCESS FOR SYNTHESIZING MAGNESIUM BORATE NANORODS AND ITS MECHANICAL PROPERTY FOR REINFORCED NYLON-6 COMPOSITES

LICONG WANG,YUAN LIU,YUSHAN ZHANG,DAN CHEN,YUQI WANG,ZELIANG DONG,YONGCHAO LU,XIPING HUANG 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2014 NANO Vol.9 No.7

Magnesium borate (Mg 2 B 2 O 5 Þ nanorods were synthesized by a two-step process, includingsolution-chemical technology and a ternary-°ux method, using concentrated seawater and H 3 BO 3as raw materials. X-ray di®raction (XRD) showed that the sample had triclinic structure. Scanning electron microscopy (SEM) and high resolution transmission electron microscopy (HR-TEM) indicated that it consisted of rod-like particles with an average diameter of 100 – 150 nmand length over 5 ? m. Di®erential thermal analysis (DTA) con¯rmed that the melting point ofthe ternary-°ux and the formation temperature of Mg 2 B 2 O 5 were lower than single-°ux process. The formation of Mg 2 B 2 O 5 nanorods was more e±cient by ternary-°ux than single-°ux. Me-chanical property of Mg 2 B 2 O 5 nanorods reinforced Nylon-6 composites showed that KH550 wasthe optimal coupling agent and made the strength of the composites to be improved to differentdegrees.

FK866 inhibits colorectal cancer metastasis by reducing NAD+ levels in cancer-associated fibroblasts

Xie Hanhan,Lei Yun,Mao Yushan,Lan Jingbin,Yang Jing,Quan Hui,Zhang Tao 한국유전학회 2022 Genes & Genomics Vol.44 No.12

Background: Extraintestinal metastasis is the main therapeutic challenge for colorectal cancer, the third most common cancer worldwide. Various components of the tumor microenvironment, especially cancer-associated fibroblasts (CAFs), play important roles in tumor metastasis. NAMPT is often overexpressed in tumor tissues and is associated with poorer prognosis. However, the specific roles of NAMPT as well as NAD+ in tumor metastasis are relatively unknown. Therefore, we investigated the role of NAMPT and related NAD+ metabolism in cancer-associated fibroblasts mediated colorectal cancer metastasis. Objective: This study sought to explore the molecular mechanism of FK866 in CAFs cell and colorectal cancer proliferation and metastasis. Methods: The expression of NAMPT in clinical tissues were detected by immunohistochemically analysis. To investigate the role of NAMPT and NAD+ in the interactions between cancer cells and cancer-associated fibroblasts in tumor microenvironment, we isolated CAFs from normal and cancer tissues of clinical colorectal cancer patients. CAFs were treated with different concentrations of FK866, inhibitor of NAMPT, then the NAD+ content was detected using kits, the expression of CAFs activity and stemness indexes was assessed by Western blot and immunofluorescence. The secreted factors of these cells were analyzed by cellular inflammatory factor microarrays. The migration of SW480 after co-cultured with FK866-treated CAFs was detected by Transwell. Finally, high-throughput sequencing was performed to identify the proteins that are associated with the effect of altered NAD+ in CAFs on the migration of cancer cells. Results: NAMPT expression is significantly higher in colorectal cancer tissues, especially in metastatic cancer patients, than that in normal tissues. Inhibition of NAMPT by FK866 in CAFs decreases the expression of activity indicators (α-SMA, PDGFRβ), stemness indicators (BMI-1, OCT4), inflammatory factors and chemokines. Meanwhile, FK866 treatment inhibits the migration ability of SW480 cells co-cultured with CAFs. Finally, high-throughput sequencing reveals that PITX3 are down-regulated after NAD+ reduction in CAFs, which could be reversed by adding NAM, a raw material for NAD+ synthesis. Conclusion: Inhibition of the NAMPT-mediated NAD+ synthesis by FK866 may decrease the activation and stemness of CAFs, reduce the secretion of inflammatory and chemokines by suppressing the expression of PITX3, resulting in the suppression of colorectal cancer metastasis.