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- 저자 : Xuyan Tang (검색결과 2 건)
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Effect of Plasma-Treated Polymer Substrates on Fabricating Surface Microsystems Through LbL Coating
Xuyan Liu,Shen Tang,최홍기,최호석 한국고분자학회 2010 Macromolecular Research Vol.18 No.5
The surface free energy of three polymeric substrates, PMMA (poly(methyl methacrylate)), PS (polystyrene)and PC (polycarbonate), subjected to a RF O2 plasma treatment and washing with water was studied with the aim of fabricating surface micro-systems using a LbL technique. More uniform hysteresis of the deionized(DI) water contact angle after the plasma treatment followed by washing with water was observed on the PS surface compared to that on the PMMA and PC surfaces. XPS showed that washing with water may alter the plasma generated functionality,which readily forms oxygen containing groups, such as C=O and O-C=O. The surface free energy measured by the acid-base method showed that the Lewis base components on PS were less affected than those on PMMA and PC, due to the weak solvent effect of the former. During the LbL coating of the polyelectrolyte, the solvent effect was less likely to occur on PS, which exhibited better electrostatic bonding of the positive and negative polyelectrolytes (PEL). The fluorescence intensity was similar to the above findings, which may help determine the best substrate for surface micro-patterning using the LbL technique.
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Yong Zhou,Kuang Gu,Fengying Sun,Shoumei Xuan,Duohong Zou,Jiacai He,Xuyan Tang 한국조직공학과 재생의학회 2022 조직공학과 재생의학 Vol.19 No.4
BACKGROUND: Regenerative medicine by using stem cells from dental pulp is promising for treating patients with critical limb ischemic (CLI). Here, we investigated the difference in the angiogenetic ability of stem cells from human exfoliated deciduous teeth (SHED) and human dental pulp stem cells (DPSC). METHODS: SHED and DPSC were harvested from dental pulp and analyzed in flow- cytometry for detecting the expression of surface markers. Levels of angiogenetic marker were examined by RT-PCR and Western-blot. Eighteen immunodeficient mice of critical limb ischemic model were divided into three groups: SHED, DPSC and saline, which was administered with SHED, DPSC or saline intramuscularly. Histological examination was performed to detect the regenerative results. RESULTS: A highly expression of CD146 was detected in SHED. Moreover, cells with negative expression of both CD146 and CD31inSHEDwere more in comparison with those inDPSC. Expression of angiogenesis factors includingCXCL12,CXCR4,Hif- 1a, CD31, VEGF and bFGFwere significant higher in SHED thanDPSC by the RT-PCR and Western-Blot results. SHED induced more CD31 expression and less fibrous tissue formation in the critical limb ischemic model as compare with DPSC and saline. CONCLUSION: Both SHED and DPSC possessed the ability of repairing CLI. With expressing more proangiogenesis factors, SHED may have the advantage of repairing CLI.
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