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Tests of the interface between structures and filling soil of mountain area airport
Wu, Xueyun,Yang, Jun Techno-Press 2017 Geomechanics & engineering Vol.12 No.3
A series of direct shear tests were conducted to investigate the frictional properties of the interface between structures and the filling soil of Chongqing airport fourth stage expansion project. Two types of structures are investigated, one is low carbon steel and the other is the bedrock sampled from the site. The influence of soil water content, surface roughness and material types of structure were analyzed. The tests show that the interface friction and shear displacement curve has no softening stage and the curve shape is close to the Clough-Duncan hyperbola, while the soil is mainly shear contraction during testing. The interface frictional resistance and normal stress curve meets the Mohr-Coulomb criterion and the derived friction angle and frictional resistance of interface increase as surface roughness increases but is always lower than the internal friction angle and shear strength of soil respectively. When surface roughness is much larger than soil grain size, soil-structure interface is nearly shear surface in soil. In addition to the geometry of structural surface, the material types of structure also affects the performance of soil-structure interface. The wet interface frictional resistance will become lower than the natural one under specific conditions.
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Xueyun Wu,Zhengbin Lv,Yaozhou Zhang,Dong Yang,Xiangcheng Zhu,Zhinan Xu,Zhiyang Feng,Ben Shen 한국생물공학회 2010 Biotechnology and Bioprocess Engineering Vol.15 No.4
The heterologous production of iso-migrastatin (iso-MGS) was successfully demonstrated in an engineered S. lividans SB11002 strain, which was derived from S. lividans K4-114, following introduction of pBS11001,which harbored the entire mgs biosynthetic gene cluster. However, under similar fermentation conditions, the iso-MGS titer in the engineered strain was significantly lower than that in the native producer - Streptomyces platensis NRRL 18993. To circumvent the problem of low iso-MGS titers and to expand the utility of this heterologous system for iso-MGS biosynthesis and engineering, systematic optimization of the fermentation medium was carried out. The effects of major components in the cultivation medium,including carbon, organic and inorganic nitrogen sources,were investigated using a single factor optimization method. As a result, sucrose and yeast extract were determined to be the best carbon and organic nitrogen sources, resulting in optimized iso-MGS production. Conversely, all other inorganic nitrogen sources evaluated produced various levels of inhibition of iso-MGS production. The final optimized R2YE production medium produced iso-MGS with a titer of 86.5 mg/L, about 3.6-fold higher than that in the original R2YE medium, and 1.5 fold higher than that found within the native S. platensis NRRL 18993 producer.
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