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Yue-Feng Wang,Xue-Yue Hou,Chuan-Ying Jiang,Tong-Tong Zhai,Rui Miao,Jun-Jie Deng,Zhi-Hong Yao,Rongshu Zhang 한국원예학회 2021 Horticulture, Environment, and Biotechnology Vol.62 No.2
Trichoderma spp. are widely applied, eco-friendly mycofungicides and plant growth promoters. Native Trichoderma strainsare likely to have more productive, stable biocontrol and biofertilizer activities since they are well adapted to the local environment. In this study, we isolated a native Trichoderma strain ‘Th62’ from the rhizosphere soil of wild Chelidonium majusplants in Harbin, China (126.6341°E, 45.7242°N). The isolated Trichoderma strain was identifi ed as a T. harzianum strainvia morphological observation and molecular methods based on the rDNA internal transcribed spacer region and elongationfactor-1α gene sequences. Signifi cant antagonistic activities of Th62 against fi ve soil-borne fungal phytopathogens,Fusarium oxysporum , Sclerotinia sclerotiorum , Alternaria alternata , Cytospora chrysosperma, and Rhizoctonia solani ,were confi rmed by dual-culture assays. Furthermore, the crude fermentation products of Th62 also displayed antifungalactivities against these fi ve pathogens. To evaluate the function of Th62 as a biofertilizer, we subsequently applied Th62on cockscomb ( Celosia cristata L), a plant species with both ornamental and medicinal values, by inoculation with Th62conidia at diff erent concentrations, 1 × 10 10 cfu mL −1 , 1 × 10 11 cfu mL −1 , and 1 × 10 12 cfu mL −1 . The benefi cial eff ects ofTh62 were evaluated by measuring the growth and photosynthetic traits of the inoculated cockscomb plants, and the resultsdemonstrated that Th62 signifi cantly improved the photosynthetic effi ciency, photosynthetic capacity, and the adaptabilityto intense light of the inoculated cockscomb plants compared to the controls. Consistently, Th62 inoculation signifi cantlyimproved the growth and fl ower yield of cockscomb. We presented a positive case of isolating and applying native microbialresources on local plantation practices.
Serum Proteomics Analysis of Feline Mammary Carcinoma Based on Label-free and PRM Techniques
Jia-San Zheng,Ren-Yue Wei,Zheng Wang,Ting-Ting Zhu,Hong-Ri Ruan,Xue Wei,Kai-Wen Hou,Rui Wu 대한수의학회 2020 Journal of Veterinary Science Vol.21 No.3
Background: Feline mammary carcinoma is the third most common cancer that affects female cats. Objectives: The purpose of this study was to screen differential serum proteins in feline and clarify the relationship between them and the occurrence of feline mammary carcinoma. Methods: Chinese pastoral cats were used as experimental animals. Six serum samples from cats with mammary carcinoma (group T) and six serum samples from healthy cats (group C) were selected. Differential protein analysis was performed using a Label-free technique, while parallel reaction monitoring (PRM) was performed to verify the screened differential proteins. Results: A total of 82 differential proteins were detected between group T and group C, of which 55 proteins were down regulated and 27 proteins were up regulated. Apolipoprotein A-I, Apolipoprotein A-II (ApoA-II), Apolipoprotein B (ApoB), Apolipoprotein C-III (ApoC-III), coagulation factor V, coagulation factor X, C1q, albumen (ALB) were all associated with the occurrence of feline mammary carcinoma. Differential proteins were involved in a total of 40 signaling pathways, among which the metabolic pathways associated with feline mammary carcinoma were the complement and coagulation cascade and cholesterol metabolism. According to the Label-free results, ApoB, ApoC-III, ApoA-II, FN1, an uncharacterized protein, and ALB were selected for PRM target verification. The results were consistent with the trend of the label-free. Conclusions: This experimen is the first to confirm ApoA-II and ApoB maybe new feline mammary carcinoma biomarkers and to analyze their mechanisms in the development of such carcinoma in feline.
Risk of venous thromboembolism in Chinese pregnant women: Hong Kong venous thromboembolism study
Duo Huang,Emmanuel Wong,Ming-Liang Zuo,Pak-Hei Chan,Wen-Sheng Yue,Hou-Xiang Hu,Ling Chen,Li-Xue Yin,Xin-Wu Cui,Ming-Xiang Wu,Xi Su,Chung-Wah Siu,Jo-Jo Hai 대한혈액학회 2019 Blood Research Vol.54 No.3
BackgroundPrevious Caucasian studies have described venous thromboembolism in pregnancy; however, little is known about its incidence during pregnancy and early postpartum peri-od in the Chinese population. We investigated the risk of venous thromboembolism in a “real-world” cohort of pregnant Chinese women with no prior history of venous thromboembolism.MethodsIn this observational study, 15,325 pregnancies were identified in 14,162 Chinese women at Queen Mary Hospital, Hong Kong between January 2004 and September 2016. Demographic data, obstetric information, and laboratory and imaging data were retrieved and reviewed.ResultsThe mean age at pregnancy was 32.4±5.3 years, and the median age was 33 years (interquartile range, 29‒36 yr). Pre-existing or newly diagnosed diabetes mellitus was present in 627 women (4.1%); 359 (0.7%) women had pre-existing or newly detected hypertension. There was a small number of women with pre-existing heart disease and/or rheumatic conditions. Most deliveries (86.0%) were normal vaginal; the remaining were Cesarean section 2,146 (14.0%). The incidence of venous thromboembolism was 0.4 per 1,000 pregnancies, of which 83.3% were deep vein thrombosis and 16.7% were pul-monary embolism. In contrast to previous studies, 66.7% of venous thrombosis occurred in the first trimester.ConclusionChinese women had a substantially lower risk of venous thromboembolism during preg-nancy and the postpartum period compared to that of Caucasians. The occurrence of pregnancy-related venous thromboembolism was largely confined to the early preg-nancy period, probably related to the adoption of thromboprophylaxis, a lower rate of Cesarean section, and early mobilization.