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Xuhong Zhou,Xijun Mo,Suping Qu,Min Tian,Xue Wei Wu,Min Wu,Jinze Li,Ying Luo,Jihua Wang,Min Gui 한국원예학회 2014 Horticulture, Environment, and Biotechnology Vol.55 No.2
Double haploids in lisianthus cultivars were produced using anther culture. The anther of ‘Art Peach’ wascultured on the Murashige and Skoog (MS) medium with 3% sucrose with auxin and cytokinin. The highest frequencyof calli developed from anthers (100%) and green plantlets per 100 calli (60 regenerants) was observed on the mediumcontaining 8 mg・L-1 N6-benzyladenine (BA) and 0.1 mg・L-1 naphthaleneacetic acids (NAA). When anthers were culturedon the medium supplemented with 2 mg・L-1 BA and 0.5 mg・L-1 NAA, 33.3% of calli formed plantlets. Using theanther culture, callus induction and plantlets regeneration in five cultivars were developed differently, suggesting thatthe genotype might predetermined anther culture response. These results indicate that a highly efficient new microprogationtechnique can be provided by anther culture in vitro for lisianthus. Anther culture in vitro can provide a highly efficientnew microprogation technique for lisianthus.