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RISS 인기검색어
- 검색키워드
- 저자 : Xie Yihui (검색결과 2 건)
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Separating paint mist from paint spraying waste gas: mechanism, model and application
Yihui Zhou,Xi He,Wang Li,Tao Xu,Dong Xie,Xin Dai,Zhang Liu,Hanqing Wang,Gang Yu 대한환경공학회 2021 Environmental Engineering Research Vol.26 No.5
The paint spraying waste gas contains a large amount of paint mist, volatile organic compounds (VOCs) and other hazardous materials, which seriously affects human health and atmospheric environment. Paint mist cannot be separated from paint spraying waste gas effectively by the existing dry filtration methods because of the filter blocking. A new separation process and a novel coupling device with high separation efficiency have been developed. A numerical model was established to optimize the structure and operation parameters of the baffle interceptor by hydrodynamics method which verified by the coupling device experiment. From the simulating results, when plate spacing was 20 ㎜, folding angle was 90° and airflow velocity was up to 8 ㎧, the separation efficiency of paint mist with the diameter of 15 ㎛ was 84.7% and the pressure drop of the baffle interceptor was 220.2 ㎩. Experiment was conducted under the above-optimized conditions, and the results showed that the pressure drop of the baffle interceptor was 303.33 ㎩, and the paint mist separation efficiencies of the baffle interceptor and the interception-filtration coupling device were 78.34% and 96.38%, respectively.
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The cytoplasmic loops of AgrC contribute to the quorum-sensing activity of Staphylococcus aureus
Huang Qian,Xie Yihui,Yang Ziyu,Cheng Danhong,He Lei,Wang Hua,Liu Qian,Li Min 한국미생물학회 2021 The journal of microbiology Vol.59 No.1
In Staphylococcus aureus, the accessory gene regulator (agr) quorum-sensing system is thought to play an important role in biofilm formation. The histidine kinase AgrC is one of the agr system components and activated by the self-generated auto-inducing peptide (AIP), which is released continuously into the extracellular environment during bacterial growth. The extracellular loops (Extra-loops) of AgrC are crucial for AIP binding. Here, we reported that the cytoplasmic loops (Cyto-loops) of AgrC are also involved in Agr activity. We identified S. aureus ST398 clinical isolates containing a naturally occurring single amino acid substitution (lysine to isoleucine) at position 73 of an AgrC Cyto-loop that exhibited significantly stronger biofilm formation and decreased Agr activity compared to the wild-type strain. A constructed strain containing the K73I point mutation in AgrC Cyto-loop continued to show a growth dependent induction of the agr system, although the growth dependent induction was delayed by about 6 h compared to the wild-type. In addition, a series of strains containing deletion mutants of the AgrC Cyto- and Extra-loops were constructed and revealed that the removal of the two Cyto-loops and Extra-loops 2 and 3 totally abolished the Agr activity and the growth-dependence on the agr system induction. Remarkably, the Extra-loop 1 deletion did not affect the Agr activity. In conclusion, the AgrC Cyto-loops play a crucial role in the S. aureus quorum-sensing activity.
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